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Plant Breeding: Quiz 3
University of Guelph MBG*4160
94
Microbiology
Undergraduate 4
11/18/2015

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Term
Anther culture
Definition
A method of obtaining haploids in the doubled haploid method. Used in tobacco and rice.
Term
Artificial hybridizaiton
Definition
Involved emasulation of the female parent and hand-pollination.
Term
Art
Definition
Creation of something from your imagination. Plant breeding is an art.
Term
Atlas
Definition
The highest yielding variety in the mixture in the experiments by Suneson and Wiebe.
Term
Backcross
Definition

A4*/B

A type of cross. The donor and recurrent parents are crossed, and the progeny crossed to the recurrent parent for multiple generations.

Advantages: Requres few plants, known and desirable recurrent parent phenotype is recovered, rapid, predictable, repeatable, breeding program can be independent of the environment.

Disadvantages: does not permit selection of new combinations of genes, linkage drag may be a problem.

Term
Background selection
Definition
Marker-assisted back-crossing used to recover the parent genome.
Term
Base
Definition
In the RIPE system, populations which are 50% elite.
Term
Bergner
Definition
Proposed the double haploid method in 1924 after studying Jimson weed.
Term
Bi-Parental crosses
Definition
Backcrosses, bulk, pedigree, single seed descent. Limited in their ability to combine genetic traits from several sources.
Term
Bulk method
Definition

A method of selection for self-pollinated crops following a cross, with no early generation selection. Developed by Nillson-Ehle. The simplest line development method. Progeny testing is performed only after acceptable homozygosity is achieved.

Advantages: No record keeping until F4, low labour, allows natural selection (biotic, abiotic, competition)

Disadvantages: Natural selection is not always an advantage: progeny are lost due to early sampling.

1. Cross parents to obtain F1.

2. Allow F1 to self-pollinate to produce F2.

3. Grow 1000 F2 plants, select the best plants or allow natural selection. Obtain a bulk of F3 seeds.

4. Grow 1000 F3 plants, select the best plants or allow natural selection. Obtain a bulk of F4 seeds.

5. Grow 1000 F4 plants, select best plants or allow natural selection to occur. Make single plant selections for F5 seeds.

6. First year trials of 100 F5 lines at 1 - 3 locations. Select the best 20 plants, save selfed seed.

7. Second year trials of 20 F6 lines at many locations. Select the best 5 plants, save selfed seed.

8. Third year trials of 5 F7 lines at many locations. Release a new cultivar.

 

 

Term
Business
Definition
Plant breeding is a business. Many corporations are involved in the profitable enterprise of plant breeding.
Term
Chain cross
Definition
A type of cross. Produces 8 different progenies.
Term
Choosing objectives
Definition
The first step in developing a self-pollinated cultivar. Consider current and future markets. Is the product the seed, seed component, plant, or specific compound. What is the environment? Increase productivity or quality.
Term
Chromosome elimination
Definition

Hordeum bulbosum method

A method of obtaining haploids in doubled haploid method.

Term
Colchicine
Definition
Used in double haploid method to double the number of chromosomes in haploid plantlets.
Term
Conical cross
Definition

A/B//C/D/3/E/F//G/H

A type of cross. A cross of two double crosses.

Term
Crop Science
Definition
A journal which used to publish cultivar registrations. Created the Journal of Plant Registrations.
Term
Cultivar
Definition
A plant population selected for desirable characteristics that can be maintained by propagation. Must have identity and reproducibility.
Term
Donor parent
Definition
In back-crossing, the parent with the desired gene. It is crossed only once into the backcross, at the beginning.
Term
Double cross
Definition

A/B//C/D

A type of cross. Two sets of parents are crossed, and the progeny crossed.

Term
Doubled haploid
Definition

A method of selection for self-pollinated crops proposed by Bergner. Theoretical until the development of in vitro systems in the 1970's. Follows a cross, with no early generation selection. Haploid plantlets are produced by ovary culture, anther culture, chromosome elimination, microspore culture, or in vivo systems (spontaneous, semigamy, and polyembryony). Chromosomes are doubled by cochicine to produce homozygosity.

Advantages: Quick, small population size, doubled haploids are extremely uniform, selection is very efficient, favourable linkages are preserved.

Disadvantages: Specialized personnel and infrastructure are required, efficiency depends on genotype, and unfavourbale linkages are preserved.

1. Cross parents to obtain F1.

2. Use lab methods to double the chromosome number in F1 to produce doubled haploid F2.

3. Grow F3 plants and evaluate and select: homozygosity is achieved.

 

Term
Early generation testing
Definition
Evaluation of lines at an early generation of selfing so that further phenotyping can be focused on lines that are most promising.
Term
East
Definition
In 1916, studied the effect of selection during inbreeding on the tobacco corolla tube length. Found that the longer you inbreed with selection, the more plants have phenotypic values close to parental values. F1 will be an intermediate between parents, but homogenous. F2 will have greater variation than F1 or the parents. Segregation and recombination occurrs.
Term
Elite
Definition
In the RIPE system, populations which are 93.25% elite.
Term
Emasculation
Definition
A part of artificial hybridization. Removal of the anthers from the female parent.
Term
Evaluating and selecting superior homozygous lines
Definition
The fifth step of developing a self-pollinated cultivar.
Term
Experimental strains
Definition
Varieties that are not released to the public, only used in breeding programs.
Term
F1
Definition
The product of a cross between two parents. If the parents are homozygous, F1 is heterozygous, and the population is homogenous.
Term
F2
Definition
The progeny of F1. Heterozygous and heterogeneous.
Term
Fixed genes
Definition
Genes which are the same in both alleles. F1 is homozygous for these genes.
Term
Food security
Definition
A reason for plant breeding. Food is a human right. Protects the right of people to feed themselves with dignity: sufficient food that meets dietary needs is available with means to access it.
Term
Foreground selection
Definition
Marker-assisted back-crossing used to track the gene being introgressed.
Term
Formation of segregating populations
Definition
Variation can be obtained by introducing a new population, mutations, somaclonal variation, plant transformation, or hybridization.
Term
Goulden
Definition
Proposed single seed descent method in 1939 for use in wheat.
Term
Harlan and Martini
Definition
In 1983, studied barley and found that some plants could be favoured in one environment and out-competed in another. Studied varieties in ten locations for 4 - 12  years.
Term
Heterogeneous
Definition
Populations are genetically diverse.
Term
Heterozygous
Definition
Having two different alleles at a locus.
Term
High
Definition
In the RIPE system, populations which are 87.5% elite.
Term
Homogenous
Definition
Populations are genetically uniform.
Term
Homozygous
Definition
Having two of the same alleles at a locus.
Term
Hybridization
Definition
The most common method of producing new variation in a population. Parents must differ genetically for traits of interest.
Term
Ideotype
Definition
The target phenotype of plant breeding.
Term
Inbreeding to an adequate level of homozygosity
Definition
The fourth step of developing a self-pollinated cultivar. The numbers of generations of inbreeding before selection, and management method during inbreeding must be decided.
Term
Intermediate
Definition
In the RIPE system, populations which are 75% elite.
Term
Introductions
Definition
In the RIPE system, populations which are 0% elite.
Term
Jimson weed
Definition
Studied by Bergner in 1924, in experiments that led him to propose the doubled haploid method.
Term
Johannsen
Definition
Postulated the Pure Line Theory.
Term
Journal of Plant Registration
Definition
Created by Crop Science in 2006. Publishes cultivar registrations.
Term
Landrace
Definition
Heterogeneous, homozygous. In the 1930's they selected varieties from landraces.
Term
Line
Definition
Derived from a single plant selection.
Term
Marker-assisted back-crossing
Definition
Backcrossing using molecular markers to phenotype. Used for foreground selection or background selection.
Term
Mass selection
Definition

A method of selection for self-pollinated crops using existing variation. Plants are chosen from a genetically diverse population on the basis of phenotype. Seed is composited without progeny testing. The easiest method of selection in self-pollinating crops. The end product doesn't differ in performance from the original population, but it is more uniform. Develop a new culivary by improving average performance of the population.

Objectives: Purify a mixed cultivar or plant population by propagating plants with similar phenotype, and develop a new cultivar by improving average performance of the population.

Term
Microspore culture
Definition
A method of obtaining haploids in doubled haploid method.
Term
Modified bulk-pedigree
Definition
A method of selection for self-pollinated crops following  across, with early generation selection.
Term
Modified single-seed descent
Definition

Similar to SSD, but instead of one, 2 - 3 seeds per plant are used for the following generation. Progeny from individual lines is maintained separately during each generation of increase.

Advantages: Reduces selection efforts prior to F6, easy, no natural selection, well suited to winter/off-season nurseries.

Disadvantages: More plants than SSD, no elimination of undesirable alleles early on, no natural selection, genetic combinations may be lost if population sizes are too small.

Term
Molecular marker
Definition
A polymorphism in the DNA. Can be a size difference, or a sequence difference.
Term
Natural selection
Definition
In bulk selection, plants that are most adapted to growing conditions are naturally selected. This effect is not neglicible, as shown by experiments by Suneson and Wiebe.
Term
Near isogenic line (NIL)
Definition
The result of a backcross. It is identical to the recurrent parent, except for one desired gene which was introgressed in from the donor parent.
Term
Nilsson-Ehle
Definition
Developed bulk method in Svalof, Sweden in 1908.
Term
Ovary culture
Definition
A method of obtaining haploids in doubled haploid method.
Term
Pedigree selection
Definition

A method of selection for self-pollinated crops, following a cross, with early generation selection. Follows hybridization. Progeny testing is an essential feature. Breeder maintains a family structure by growing progeny of each selected single plant in progeny rows. Single plant selections in early generation, and row selection when homozygosity is reached. Was popular in the 80's and 90's, but was replaced with bulk method.

Advantages: Traces the lines of each cultivar back to an F2 plant (allows breeders to exploit variation within prior generations), artificial selection can be imposed in each generation, breeder can use their "breeder eyes".

Disadvantages: Additional work is needed to keep track of pedigree lines, more resources are required.

1. Cross parents to obtain F1.

2. Allow F1 to self-pollinate to obtain F2.

3. Grow 1000 F2 plants. Screen for traits with high heritability. Save selfed seeds from 200 plants.

4. Grow 200 F3 lines in progeny rows. Select the best 50 lines. Save selfed seeds from the best 1 - 2 plants in each row.

5. Grow 50 - 60 F4 lines and sublines. Select the best 15 lines. Save selfed seeds from the best 1 - plants in each row.

6. Trials of 15 T5 lines at 2 - 3 locations. Select the best 8 lines. Saved self seeds.

7. Trials of 8 F6 lines at more locations. Select the best 4 lines. Select selfed seeds.

8. Trials of 4 F7 lines at more locations. Release new cultivars.

Term
Phenotypic back-cross breeding
Definition
Standard procedure to introgress major genes into other germplasm. The donor parent and recurrent parent are crossed, and back-crossed with the recurrent parent, resulting in a near-isogenic line. If the gene is recessive, progeny testing is required after every generation.
Term
Plant breeding
Definition
The genetic improvemnet of plants for human benefit. A science, art, business, and technology. Food security is important. An act of throwing things away: shifts the population into the right direction.
Term
Plantlets
Definition
Haploid plants produced in doubled haploid method.
Term
Pollination
Definition
Transfer of pollen from the male parent to the stigma of the female parent. A part of artificial hybridization.
Term
Polyembryony
Definition
An in vivo mthod of obtaining haploids in doubled haploid method. Used in peppers and asparagus.
Term
Preparing seed stocks for commercial distribution
Definition
The sixth step of developing a self-pollinated cultivar.
Term
Princess
Definition
The variety of common bean that Johannsen used in his experiments.
Term
Progeny testing
Definition
Determining a plant's genotype by looking at its progeny. Plant a row of each of the progeny.
Term
Pure line
Definition
Homozygous, homogenous. Progeny descended from one self-pollinated, homozygous plant.
Term
Pure line selection
Definition

A method of selection for self-pollinated crops using existing variation. Isolating pure lines from a mixed population.

1. Grow 1000 plants, screen for traits with high heritabilty, save selfed seeds from 100 selected plants.

2. First year trials of 100 pure-lines at 1 - 3 locations. Select the best 20 pure lines.

3. Second year trials of 20 pure lines at many locations. Select the best 5 pure lines.

4. Third year trials of 5 pure lines at many locations. Release new cultivars.

Term
Pure Line Theory
Definition
The action of selection cannot go beyond known limits - it must stop when the purification of the most strongly divergent line is complete. Postulated by Johannsen, based on his work selecting for high and low seed weight in Princess common beans. After the first cycle of selection within the variety, there was no change in seed weight. All variation is from environmental interactions alone.
Term
Pyramid cross
Definition

Convergent cross

A type of cross. All possible ways of a conical cross between 8 parents is tried for 8 sets of F1.

Term
Recombinant inbred lines (RIL)
Definition
Inbred, homozygous lines.
Term
Recurrent Introgressive Population Enrichment system (RIPE)
Definition
Male-sterility faciliated recurrent selection in barley. There are introductions, base, intermediate, and high populations. A male sterility allele is employed, tightly linked in coupling phase with the sex 1 locus. Genotype has shrunken in the endosperm: all shrunken seeds are male-sterile. Only the male-fertile seeds are used and selected during inbreeding phase. Large numbers of crosses can be made with ease because emasculation is unneccessary. 500 - 700 crosses can be made in a week. Introduces a broad range of germplasm.
Term
Recurrent parent
Definition
In a backcross, the parent that lacks the desired gene, but may be an elite variety. It is backcrossed many times in the backcross.
Term
Red 5
Definition
The first variety of wheat released. Developed in Ontario. Brought from Scotland by a family of immigrants. It was a winter wheat but they planted it in the spring. Only one plant came up, and it became the ancestor of all spring wheat in Canada. A pure line.
Term
Repeated inter-mating
Definition
Can combin traits from several sources. Breaks up linkages among groups of loci, enabling a new genetic combinations.
Term
Response to selection (RS)
Definition
Genetic gain.
Term
Science
Definition
Testing a hypothesis to generate new knowledge. Plant breeding is a science.
Term
"Seed morgue"
Definition
A term used to describe seed banks, because breeding programs tend to avoid non-elite germplasm.
Term
Segregation
Definition

The portion of individuals in a population which are heterozygous.

= (½)m

m = the number of generations of self-fertilization = (generation number - 1)

Term
Selecting parents
Definition
The second step in developing a self-pollinated cultivar. Depends on the objectives.
Term
Selection intensity
Definition
The portion of the population which is selected. Usually 10% - 20%.
Term
Semigamy
Definition
An in vivo method of obtaining haploids in doubled haploid method. Used in cotton.
Term
Simple cross
Definition

A/B

A type of cross. Two parents are crossed.

Term
Single seed descent (SSD)
Definition

A method of selection for self-pollinated crops proposed by Goulden. Follows a cross, with no early generation selection. A form of bulk method. Progeny testing is postponed until acceptable homozygosity is reached. Selections can be as good as other systems.

Advantages: Each F2 plant is represented in the progeny test. The varietion of F2 is well represented, and advese effect of natural selection is minimized. Not much space is required: possible to grow multiple generations indoors.

Disadvantages: No selection during early generations, harvest may be more difficult than conventional bulk.

1. Cross parents to obtain F1.

2. Allow F2 to self-pollinate to produce F2.

3. Grow 1000 F2 plants. Save one seed from each of 150 selected plants. Bulk the F3 seed.

4. Grow 150 F3 plants. Save one seed from each plant. Bulk the F4 seeds.

5. Grow 150 F4 plants. Save F5 seeds from 100 individual plant selections. 

6. First year trials of 100 F5 lines at 1 - 3 locations. Select the best 20 plants. Save selfed seeds.

7. Seocnd year trials of 20 F6 lines at many locations. Select the best 5 lines. Save selfed seeds.

8. Third year trials of 5 F7 lines at many locations. Release a new cultivar.

Term
Sister lines
Definition
Two varieties that are from the same cross. Can be very different.
Term
Spontaneous
Definition
An in vivo method of obtaining haploids in doubled haploid method. Used in maize.
Term
Spreader row
Definition
A row of disease-susceptible variety planted next to a line in a breeding program. Imposes a disease selection pressure on the population. Mist irrigation may also impose a disease selection pressure.
Term
Suneson and Wiebe
Definition
Did experiments in 1942 that showed that natural selection had considerable effect in bulk method selection. Atlas was the highest yielding variety in the mixture.
Term
Technology
Definition
Physical application of knowledge. A new variety is a new technology.
Term
Tree-way cross
Definition

A/B//C

A type of cross. Two parents are crossed, and the progeny are crossed to a third parent.

Term
Variety
Definition
A plant population with identity and reproducibility, but is not cultivated. A subdivision of species, for taxonomic classification. A group of indivual plants that are genetically distinct from other groups of plants within the species.
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