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Plant Breeding: Final Exam
University of Guelph PLNT*6340
70
Agriculture
Undergraduate 4
12/09/2020

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Term
Domestication traits
Definition
- Non-shattering of seeds

- Synchronous flowering

- Larger fruit and seed

- Loss of defensive structures (hair, thorns...)

- Loss of poisonous substances
Term
Planned domestication steps (Lupinus example)
Definition
1 - Increase seed size
2 - Elimination of alkaloids
3 - Elimination of pod cracking
4 - Harder seed coat
5 - Elimination of hairs
6 - Elimination of soil water and pH sensitivity
7 - Non-branching
Term
Genetic contribution in Yield
Definition
50-70%
Term
Vavilov's Center of Origin and Diversification theory
Definition
- Center of origin would have maximum diversity

- Wild relatives can be found in the Centers of Origin
- Center of
Term
Crop movement
Definition
- When a crop is moved, the new population is a subsample of the previous, thus less diverse

- If the new area is more ecological diverse (e.g: mountains), the new population can diversify via natural selection
Term
Importance of Centers of Origin for Breeding
Definition
Sources of genetic variation
Term
Landrace
Definition
- Domesticated, locally adapted, traditional variety

- Historically maintained by local farmers
Term
Wheat domestication
Definition
Triticum urartu(AA) x Aegilops speltoides relative (BB) -> Triticum turgidum (AABB)

Triticum turgidum (AABB) x Aegilops tauschii (DD) -> Triticum aestivum (AABBDD)
Term
Germplasm
Definition
- Living tissue from which a plant can be fully recovered

- Seed or tissue culture

- Stored genetic variation

-
Term
Methods of germplasm conservation
Definition
- In situ: conservation of natural habitats and landraces - Ex situ: seed gene banks
Term
Classification of cultivated species
Definition
- Commercial varieties: developed by plant breeders. Highly productive.

- Landraces (traditional varieties)

- Breeding lines: intermediate breeding products. Narrow genetic base

- Specific gene stocks: other genetic combinations, like genomic and chromosomal mutants
Term
Classification of wild species (in terms of breeding)
Definition
- For direct use: species used by man but not cultivated. E.g. wild pastures

- For indirect use: wild species related to cultivated species, with valuable traits for crosses.

- Potentially utilizable: Not currently use but may have valuable traits.
Term
Gene pool classification
Definition
- Primary: cultivated species and immediate relatives. Can be easily crossed.

- Secondary: species which transferable genes, but with some difficulty. Progent may be sterile

- Tertiary: crosses requires some special techniques or may not be possible
Term
Canadian Plant Germplasm System (GRIN-CA)
Definition
Network of centres and people dedicated to preserving genetic diversity of crops
Term
Canada native crops
Definition
- Sunflowers
- Strawberries
- Saskatoons
- Canola
- Cranberries
Term
Qualitative traits
Definition
- Few loci
- Low environmental influence
- Phenotype can be divided into discrete classes
Term
Quantitative traits
Definition
- Many loci
- Environmental influence
- Phenotype displays continuous variation (e.g: plant height)
Term
Modes of reproduction
Definition
- Asexual (Bulb, Corm, Tubers, Rhizomes, Stolon, Apomixis)

- Sexual
Term
Apomixis
Definition
Asexual form of reproduction that produces fertile seed genetic identical to the maternal plant
Term
Types of apomixis
Definition
- Sporophytic: embryo develop from tissues external to embryo sac (adventitious embryony)

- Gametophytic Aposporic: All megaspores degenerate and embryo is develop from aposporous initial cell

- Gametophytic Diplosporic: megaspore mother cell does not undergoes meiosis and embryo is developed via parthenogenesis
Term
Pollination biology
Definition
- Self-pollinated

- Cross-pollinated
Term
Charateristics of Self-Pollinated species
Definition
- Often annual

- No inbreeding depression

- Cleistogamy

- Homozygous individuals

- Staminal sheat: Cone of anthers around carpel
Term
Charateristics of Cross-Pollinated species
Definition
- Dioecy: separate male and female plants
- Monoecy: separate male and female flowers on same plant
- Protandry: Pollen shed before stigma is mature
- Protogyny: Stigma matures before pollen is shed
- Self-incompatibility: genetic block to selfing
Term
Self-incompatibility mechanisms
Definition
- Gametophytic: genotype of pollen determines incompatibility
S1S3 x S1S3 -> no functional gametes
S1S3 x S1S2 -> S2 functional, progeny S1S2 or S3S2

- Sporophytic: genotype of parent determines incompatibility
S1S3 x S2S3 - no functional gametes
S1S3 x S2S4 - only functional combination
Term
Reciprocal cross effect
Definition
Difference in inheritance when the maternal and paternal parents are switched

- Mitochondrial -> maternal inheritance
- Plastids -> varies
Term
Phenotypic variance
Definition
Genetic variance (G) + Environmental variance (E) + Genotype x Envinronment interaction (GxE)
Term
Genetic variance
Definition
Additive variance + Breeding value + Dominance variance + Epistatic variance
Term
Heritability
Definition
Proportion of phenotypic variance due to genetic causes

- Broad sense: VG/VP
- Narrow sense: additive proportion (that can be selected)
Term
Response to selection
Definition
Gain obtained by mating selected parents

Mean of progeny - Mean of original population
Term
Genetic advance
Definition
Change in the mean of population due to 1 cycle of selection
Term
Steps in developing self-pollinated cultivar
Definition
1 - Choosing objectives
2 - Selection of parents
3 - Formation of segregating populations
4 - Inbreeding adequate levels of homozygosity
5 - Evaluate superior homozygous lines
6 - Preparing seed stocks for maket
Term
How to obtain variability?
Definition
- Introducing new populations
- Inducing mutations
- Hybridization (most common)
Term
Pure line
Definition
Product of a single self-fertilized plant

- Highly homozygous
- All plants have the same genotype (Homogeneous)
Term
Cultivar/Variety
Definition
Group of plants within a species that are genetically distinct from other groups
Term
Essential characteristic of a cultivar
Definition
- Identity: form of recognition. Morphological trait or genetic marker

- Reproducibility: progeny maintains traits
Term
Mass selection
Definition
1 - Plants are chosen for desirable phenotype

2 - Best phenotypes are selected each cycle

3 - No progeny test

4 - Retains heterozygosity
Term
Pros and Cons of Mass Selection
Definition
Pros:
- Simplest, cheapest method
- The residual heterozygosity may confer adaptations

Cons:
- Heterogeneous populations
- May not qualify for cultivar
Term
Pedigree method
Definition
Superior types are selected and a record of descent is made for each generation.

Individuals within families are selected.
Term
Pros and Cons of Pedigree method
Definition
Pros:
- Inferior and duplicate families can be discarded in early generations
- The record of descent allows the certification of cultivars

Cons:
- May narrow variability quicker
- More time consuming and costly than Mass and Bulk
Term
Bulk Method
Definition
No selection is made in early generations (~F5). And seed sowed in each generation is a sample of the previous generation

This allows natural selection to occur weeding out weaker types
Term
Pros and cons of Bulk Method
Definition
Pros:
- Saves time and labour at early gens
- Natural selection can promote locally adapted types
- More crosses can be handled

Cons:
- Natural selection may act against breeding goals
- The adapted lines may be all closely related
Term
Single Seed Descent
Definition
Advances homozygosity without selection (up to ~F5-F6)

One seed per plant is harvested to perpetuate next generation

Selection is performed from F6 and on
Term
Pros and Cons of SSD
Definition
Pros:
- The early generations can be plant in different environments unrelated to where the segregates will be grown
- Easy way to rapidly inbreed a population
- Natural selection doesn't affect populations

Cons:
- Cannot make use of natural selection
- Genetic combination may be lost if populations are too small
Term
Double Haploid
Definition
Quickest approach to homozygosity (immediate production of pure lines)

- Ovary or anther culture to produce haploids
- Duplication with colchicine
Term
Backcross method
Definition
Repeated crossing of hybrid progeny to the same variety

- Used to introgress genes
- Does not allow new combinations
- Linkage drag may be a problem
Term
Multiple Parent Strategies
Definition
- Convergent (Pyramid) cross
1x2, 3x4, 5x6, 7x8
12x34, 56x78...

- Chain cross:
1x2,2x3,3x4...

- Conical cross:
Good for cytoplasm inheritance
Term
Male-sterility
Definition
Failure of a plant to produce pollen

May be genetic engineered to facilitated crosses, without the need for emasculation
Term
RIPE: Recurrent Introgressive Population Enrichment (Male-sterile facilitated)
Definition
msg6 gene for male-sterility is closely linked to sex1 locus. Shrunken seed produces male-sterile plants.

Elite-level plants (E) are male-sterile. A population is introduced and crossed with E plants through progressive introgression.

There are 4 levels: Base (50% E), Intermediate (75% E), High (87.5% E) and Elite plants, which are 93.25%, retaining 7% of new genetic material
Term
Allele Frequency
Definition
Population: 2AA 4Aa 2aa
f(A) = (2x2+4x1+2x0)/16
f(A) = 0.5 or square root of 0.25 (AA frequency: 2/8)
Term
Selection before pollination
Definition
Eliminate inferior plants before pollination, so they will not contribute with pollen

- Gains are doubled
Term
Methods for selecting two traits
Definition
- Tandem selection: one trait improved first (C0-C5) then the other trait (C6-C10)
- Independent Culling: two traits selects simultaneously. Plot performance with 1 trait per axis.
- Selection index: formula using both traits is develop and used as base for selection
Term
Recurrent Selection
Definition
Repeated cycles of selection and intermating to improve the frequency of favorable alleles in a population
Term
Recurrent Selection Methods
Definition
- Phenotypic: selection of best performing individuals

- Half-sib family: Seed from the same plant share the same mother

- Full-sib family: Control of parents

- S1 Family: product of cross
Term
Inbreeding Depression
Definition
Loss of vigor associated to increase in homozygosity
Term
Heterosis
Definition
Increased vigor due to heterozygosity
Term
Hybrid breeding
Definition
Developing of a homogeneous population of plants from crossing inbred lines
- Heterozygous plants
- Homogeneous populations
Term
Genetic basis of Heterosis
Definition
- Dominance theory
- Overdominance theory
- Presence/Absence theory
Term
Steps in hybrid breeding
Definition
1 - Develop segregation population
2 - Inbreed population with selection
3 - Evaluate line perfomance
- General combining ability
- Specific combining ability
4 - Evaluation of potential commercial hybrids
Term
General combining ability
Definition
Considers the average performance of the progeny of individual inbreds when mated with a genetic diverse population
Term
Recurrent Selection for GCA
Definition
Recurrent selection with General combining ability as a trait
Term
Reciprocal Recurrent Selection
Definition
Recurrent selection to increase frequencies of alleles in two populations that will be crossed to produce hybrids
Term
Types of Hybrids
Definition
Single cross hybrids:
Inb 1 x Inb 2 -> F1 Hybrid

Double cross hybrid
Inb 1 x Inb 2 -> F1
Inb 3 x Inb 4 -> F1
F1 x F1 -> DC hybrid
Term
Types of Male Sterility
Definition
Cytoplasmatic: Sterile (rr) x Fertile (RR) -> Progeny 1/4 Sterile (rr) Mendelian segregation

Nuclear: Maternal cytoplasm -> Progeny 100% sterile
Term
Molecular Marker
Definition
DNA sequence associated with a location in a genome
Term
Steps for Marked Assisted Selection
Definition
1 - Develop molecular marker map
2 - Establish linkages between markers and loci controlling trait
3 - Cross and develop segregating population
4 - Genotype breeding population
5 - Predict phenotype based on markers
6 - Select molecular marker genotypes
Term
SNPs
Definition
Single Nucleotide Polymorphisms

Most used molecular marker
Term
Methods for controlling disease
Definition
1 - Resistant cultivars
2 - Crop rotation
3 - Avoid monoculture
4 - Biological control
5 - Pesticides
Term
Complete resistance
Definition
Ability of host plant to prevent any multiplication of the pathogen

- Very rare
Term
Tolerance
Definition
Cultivar develops disease but have no significant yield reduction
Term
Differential lines
Definition
Pure lines with resistance genes, used to differentiate pathogen classes
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