Term
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Definition
| The use of light or electrons to magnify objects. |
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Term
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Definition
The distance between crest to crest in the waves.
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Term
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Definition
| When a beam of light refracts or bends as it passes through a convex lens. |
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Term
| Magnification can increase? |
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Definition
| virtually w/out limit by resolution (ability to see detail) cannot increase without limit. |
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Term
| Resolution is the ability? |
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Definition
| to distinguish between two objects that are very close together. |
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Term
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Definition
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Term
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Definition
| Uses electron beams that have a much smaller wavelength than visible light. |
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Term
| The Scanning Electron Microscope |
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Definition
| Can resolve microbes and magnify them 100,000 times more than a light or Brightfield microscope that we use in our labs. |
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Term
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Definition
one lens =
magnifying glass |
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Term
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Definition
have at least two lens
(objective lens and ocular lens) |
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Term
| Our Compound Microscopes have? |
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Definition
Objective lens
Ocular lens &
a Condenser lens |
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Term
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Definition
4x=scanning objective (red)
10x=low power objective (yellow)
40x=high power objective or high and dry objective
(blue band)
100x=oil immersion objective (white band) |
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Term
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Definition
| lamp located in base of microscope that gives off visible light to illuminate specimen. |
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Term
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Definition
special lens beneath the mechanical stage that focuses rays of light to a single point on a specimen.
(Condenser can be adjusted vertically) |
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Term
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Definition
| allows the operator to move a slide around smoothly during viewing by use of stage control knobs. |
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Term
| Why do microbioligists stain there specimens? |
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Definition
to create contrast.
Staining cells increases contrast, but requires that the cells are killed.
(Killing microbes can distort specimen) |
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Term
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Definition
Stain microbial cells and increase contrast.
(Methylene blue, crystal violet, and saffranin are examples of dyes) |
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Term
Bright field microscopes
(Used to observe arrangement, shape and size of killed, stained specimens.) |
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Definition
Microbes are dark against clear, bright background.
Most common type of microscope
(viruses not seen)
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Term
Dark-Field Microscopes
(Living cells and movement) |
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Definition
Pale, small, colorless microbes are best observed.
(light hits the sides of the specimen against a dark background.) |
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Term
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Definition
King- Kingdom
Phillip- Phyllum
Came- Class
Over- Order
For- Family
Good- Genus
Sex- Species |
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Term
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Definition
3 Domain system
Archaea
Bacteria
Eukarya |
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Term
CFU
(colony forming unit) |
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Definition
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Term
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Definition
Light waves that are in phase are aligned, crest to crest and trough to trough.
Crests and troughs reinforce one another to produce a brighter image. |
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Term
Phase-contrast microscopes
Brighter = (in-phase) |
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Definition
Used to examine living microbes or specimens.
(cell movement with cilia,flagella or phagocytosis) |
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Term
Differential Interference Contrast Microscopes
(DIC)
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Definition
Nomarski microscopes
gives a detailed view of unstained, live specimens by manipulating light which makes the specimen appears as 3D. |
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Term
Photosynthetic archaea
QOT: |
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Definition
| What organism is responsible for generating the early earths atmosphere? |
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Term
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Definition
| most common group of bacteria associated with humans and animals. |
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Term
Fluorescent microscopes
(Ultra Violet radiation) |
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Definition
UV light increases resolution because it has a shorter wavelength than visible light.
Used as accurate and a quick diagnostic tool for detecting pathogens.
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Term
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Definition
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Term
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Definition
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Term
Transmission Electron Microscope
(TEM) |
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Definition
Thin, transverse sections (slice) (floor plan)
Examine fine details of internal cell structure.
Number layers of the cells envelope.
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