Term
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Definition
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Term
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Definition
| nitrogenous base + sugar + phosphate |
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Term
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Definition
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Term
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Definition
| cytosine, uracil, and thymine |
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Term
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Definition
| attached at N9 of purine ring and at N1 of pyrimidine ring by N-glycosidic linkage |
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Term
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Definition
| phosphate(acid) linked to sugar(alcohol) by a phosphoester bond |
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Term
| Ribonucleoside and Ribonucleotide of Adenine |
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Definition
| adenosine & adenosine-5'-monophosphate (AMP) |
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Term
| Ribonucleoside and Ribonucleotide of Guanine |
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Definition
| guanosine & guanosine-5'-monophosphate (GMP) |
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Term
| Ribonucleoside and Ribonucleotide of cytosine |
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Definition
| cytidine & cytidine-5'-monophosphate (CMP) |
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Term
| Ribonucleoside and Ribonucleotide of uracil |
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Definition
| uridine & uridine-5'-monophosphate (UMP) |
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Term
| Deoxyribonucleoside and Deoxyribonucleotide of adenine |
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Definition
| deoxyadenosine & deoxyadenosine-5'-monophosphate (dAMP) |
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Term
| Deoxyribonucleoside and Deoxyribonucleotide of guanine |
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Definition
| deoxyguanosine & deoxyguanosine-5'-monophosphate (dGMP) |
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Term
| Deoxyribonucleoside and Deoxyribonucleotide of cytosine |
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Definition
| deoxycytidine & deoxycytidine-5'-monophosphate (dCMP) |
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Term
| Deoxyribonucleoside and Deoxyribonucleotide of thymine |
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Definition
| deoxythymidine & deoxythymidine-5'-monophosphate (dTMP) |
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Term
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Definition
| phosphoester linkage (sugar alcohol + acid) |
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Term
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Definition
| acid anhydride linkage (acid + acid) |
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Term
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Definition
| acid anhydride linkage (acid + acid) |
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Term
| ΔG°' for hydrolysis of phosphoanhydride or acid anhydride |
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Definition
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Term
| ΔG°' for hydrolysis of phosphoester |
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Definition
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Term
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Definition
adenylate cyclase
ATP --> cAMP + 2 Pi |
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Term
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Definition
phosphodiesterase
cAMP --> AMP |
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Term
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Definition
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Term
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Definition
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Term
| How many Å separate adjacent bases along the helix axis of DNA? |
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Definition
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Term
| How many bases are in each complete turn of DNA? |
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Definition
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Term
| Repeat distance for complete turn of DNA |
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Definition
| .34 nm x 10.5 bases = 3.6 nm |
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Term
| Forces stabilizing the DNA double helix: |
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Definition
1) hydrogen bonding b/w complementary bases (specific)
2) stacking of bases in core to yield hydrophobic interactions (nonspecific) |
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Term
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Definition
| Each new dsDNA has one parental and one new strand |
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Term
| Result when you grow cells in 15N (heavy DNA), isolate DNA, and centrifuge DNA |
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Definition
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Term
| Result when you switch cells to 14N, isolate DNA of 1st generation, and centrifuge DNA |
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Definition
| one band in middle of tube |
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Term
| Result when you keep cells on 14N, isolate DNA of 2nd generation, and centrifuge DNA |
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Definition
| two bands (one greater band at top of tube and lesser band in middle of tube) |
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Term
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Definition
| denatured ssDNA has higher UV absorbance than native dsDNA |
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Term
| DNA denaturation (melting) |
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Definition
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Term
| DNA renaturation (annealing) |
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Definition
| ssDNA + complementary ssDNA --> dsDNA |
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Term
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Definition
1) heat (disrupts H bonds)
2) pH extremes (ionizes bases and disrupts H bonds)
3) decreasing salt concentration (allows phosphate groups of backbone to repell) |
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Term
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Definition
| temperature at midpoint of transition (50%) b/w ds and ss |
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Term
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Definition
-a reagent used to lower Tm of dsDNA
-used to melt dsDNA at low temps & avoid breakage or damage to sample
-disrupts H bonds b/w base pairs |
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