Term
| General characteristics of Non-Fermenting Gram Negative Bacteria |
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Definition
Strict aerobes
Non-spore forming
Carbs not used or oxidized
Do not acidify butt of TSI |
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Term
| Non-Fermenting Gram Negative Bacteria use what metabolic pathways for glucose degradation? |
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Definition
Embden-Meyerhoff (glucose degradation no oxygen)
Entner Douderoff (Use oxygen for glycolysis)
Hexose monophosphate shunt (organisms grow better without oxygen but can grow with it) |
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Term
| Clues that an organism is a Non-Fermenting Gram Negative Bacteria |
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Definition
Lack of glucose fermentation
Positive cytochrome oxidase
Fails to grow on MAC
Additional tests (Motility and flagellar arrangement. Pigment production. Nitrae reduction. Indole production. Amino acid decarboxylation) |
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Term
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Definition
Used as oxidation fermentation media.
Contains low peptones and high carbs along with bromthymol blue as a pH indicator |
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Term
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Definition
| If organism posses enyme cytochrome oxidase it can oxidize tetramethyl-p-phenylenediamine dihydrochloride to indophenol (Colored product) |
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Term
| Cytochrome oxidase: Plate method |
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Definition
| Flood plate with reagent. Look for purple color development within 30 secs |
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Term
| Cytochrome oxidase: Filter Paper Method |
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Definition
| Smear loop of orgnaism onto filter paper that is impregnated with reagent and look for purple color development within 30 secs |
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Term
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Definition
Pseudomonas aeruginosa- Pos within 3 secs
Pasteurella multocida- Pos after 30 secs
E. coli- Neg after 30 secs |
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Term
| What media enhances the formation of pyocyanin? (pigment) |
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Definition
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Term
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Definition
| Tech agar with addition of cetrimide to inhibit growth of non PSA species |
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Term
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Definition
| Classifies organisms based on the organisms' ability to utilize glucose, oxidase reactions and the ability to grow on MAC |
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Term
| Alcaligenes characteristics |
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Definition
Oxidase- Pos
Growth on MAC- Pos
Motility- Pos with peritrichous flagella |
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Term
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Definition
Inhabits soil and water
Opportunistic pathogen
Isolated from blood, sputum and urine
Has a fruity odor |
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Term
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Definition
Inhabits soil and water
Causes nosocomial pneumonia, bacteremia and meningitis
Strong glucose and xylose oxidizer |
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Term
| Flavobacterium meningosepticum (Family Cytophagaceae) |
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Definition
Involved in nosocomial infections (especially in infants)
Epidemics reported in hospital nurserues
Both blood and CSF cultures are positive |
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Term
| Flavobacterium indolgenes |
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Definition
| Cause nosocomial pneumonia seen in ICP's |
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Term
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Definition
| Characteristic fruity odor, similar to A. faecalis |
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Term
| Classification systems of Pseudomonadaceae |
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Definition
Gilardi- Based on phenotypic characteristics divided into 7 major groups
Palleroni- Based on RNA studies, divided into 5 groups |
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Term
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Definition
Fluorescent group
Produce water soluble pigment that fluoresces under UV light= pyoverdin |
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Term
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Definition
Member of the rRNA Group 1
Only member to produce pyocyanin a pigment that turns colonies green
Burn wounds, cystic fibrosis patients, acute leukemia, swimmer's ear |
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Term
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Definition
| Subgroup of rRNA 1. Soil denitrifiers that grow anaerobically. (Include ps. stutzeri whose colonies occur in soil and water and adhere to media and have a wrinkled appearance |
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Term
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Definition
Pseudomallei group
Known as the Burkholderia (resistant to polymixin B and colistin) |
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Term
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Definition
Cause of glanders in horses
Accidental in humans |
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Term
| Burkholderia pseudomallei |
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Definition
| Causes meloidosis (disease with prulent inflammation of mucus membranes and nodiles on skin which causes ulcers) |
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Term
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Definition
Phytopathogen- causes onion bulb rot in plants and foot or jungle rot in humans
Cause of pneumonia in cystic fibrosis patients |
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Term
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Definition
| Not clinically significant |
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Term
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Definition
Diminuta group
2 species are ps. dimunuta and ps. vesicularis
Pathogen in ICP's |
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Term
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Definition
Only organism is Stenotrophomonas maltophilia (used to be xanthomonas or ps. maltophilia)
Causes nosocomial infections, especially pneumonias |
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Term
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Definition
| Have a faint blue tinge on MAC |
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Term
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Definition
| Causes nosocomial infections and is saccharolytic but definitive identification is made based on the rapid production of acid from lactose oxidation |
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Term
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Definition
Assacharolytic
Biochemically inert
Rarely causes disease in humans |
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Term
| Differentiate Moraxella from M. cattharalis |
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Definition
| Penicillin elongation test |
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Term
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Definition
| Causes eye wounds, corneal ulcers and respiratory infections |
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Term
| Most commonly isolated Non-Fermenting Gram Negative Bacteria in ranked order |
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Definition
1. Pseudomonas aeruginosa
2. Acinetobacter baumanni
3. S. maltophilia
4. Burkholderia cepacia
5. Flavobacterium meningosepticum |
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Term
| Non-Fermenting Gram Negative Bacteria that are OXIDASE NEGATIVE AND NON-MOTILE |
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Definition
A. bamanni
A. lwoffi
B. parapertussis
B. pertusis |
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Term
| Non-Fermenting Gram Negative Bacteria that are OXIDASE NEGATIVE AND MOTILE |
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Definition
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Term
| Non-Fermenting Gram Negative Bacteria that are OXIDASE POSITIVE AND NON-MOTILE |
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Definition
Flavobacterium spp
Moraxella spp |
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Term
| Non-Fermenting Gram Negative Bacteria that are OXIDASE POSITIVE AND MOTILE |
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Definition
Pseudomonas spp
Alcaligenes spp
B. bronchiseptica |
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Term
| B. pertussis and B parapertussis |
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Definition
| Require special media to grow |
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Term
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Definition
Do not grow on MAC
Require increased CO2 and humidity
May take 5-7 days to grow on BAP |
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Term
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Definition
| Used for Bordetella.Prepared from potatoes which provide a high starch content that neutralizes toxic material in the specimen. Also contains sheeps blood. |
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Term
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Definition
| Use for Bordetella. Contains charcoal, horse blood cephalexin and amphotericin B |
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Term
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Definition
Causes pertussis (whooping cough)
Acquired through droplet infection and is highly contagious
Infection can be treated by erythromycin |
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Term
| Best specimen for Bordetalla pertussis |
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Definition
| Nasopharyngeal swab which then can be used for a direct fluorescent antibody test or a culture test. |
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