Term
| When trying to obtain pure cultures and control the growth of your microbes, which is better to use: complex or defined media? |
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Definition
Defined media. This kind of media can specify exactly what is in the media whereas complex media may have varying levels of nutrients |
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Term
| What is the procedure for the simple stain? |
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Definition
Simply stain the microbe using a smear and methylene blue, that's all there is to it |
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Term
| How will streptococcal bacteria be arranged under a simple stain? |
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Definition
They will be in chains of small balls |
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Term
| How will vibrio cholerae look under a simple stain? |
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Definition
They will appear as coma or vibrio |
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Term
| How will spirillum voluntans look under the simple stain? |
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Definition
They will be helical (or spirillic) |
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Term
| How would heliocbacter pylori look under the simple stain? |
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Definition
They would appear to be S-shaped |
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Term
| How would streptomyces griseus look under the simple stain? |
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Definition
They would appear to be pleomorphic |
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Term
| What will E coli, staph aureus and serratia marcescens stain under the Gram stain? |
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Definition
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Term
| How can you tell acid fast positive and acid fast negative cells apart? |
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Definition
Acid fast positive cells do not decolorize much after washing with a strong alcohol while acid fast negative bacteria lose their coloring |
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Term
| What is the acid fast stain procedure? |
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Definition
1. Smear and heat fix. 2. Apply the kinyoun primary stain. 3. Allow to stand for 10-15 minutes. 4. Rinse with water. 5. Decolorize with acid-alcohol 6. Apply methylene blue and let stand for 1 minute |
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