Term
| Colchicine continues to act until which step in the harvest? |
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Definition
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Term
| A 0.075 M solution of KCl is prepared by dissolving _____g KCl in 1000mL distilled water (KCl formula weight is 74.55) |
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Definition
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Term
The response of white blood cells to PHA: a. is independent of concentration b. begins when the culture reaches 37°C c. is an immunogenic response d. b and c e. a, b and c.
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Definition
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Term
Harvesting lymphocyte cultures for chromosome analysis includes: a. inoculating 2-4x10^6 cells into 10mL of media. b. adding 0.2mL PHA-m. c. fixing cells in a swollen state with methanol:acetic acid. d. incubating at 37°C for 48-72 hours. e. adding serum to stop the action of trypsin.
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Definition
| c. fixing cells in a swollen state with methanol:acetic acid. |
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Term
| The event separating a primary cell line from a continuous cell line is |
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Definition
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Term
| Indications of culture contamination |
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Definition
Decrease in pH of the media. Cloudy media. Poor growth without apparent reason. Visible thread-like fibers or "puff balls" |
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Term
Cells may be removed from adherence to solid support surfaces since ________
is able to _____ _____ that help to anchor the cells. |
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Definition
| Collagenase (or Trypsin); digest proteins |
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Term
| What are the two variables of greatest consequence during slide making? |
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Definition
| Temperature and humidity. |
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Term
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Definition
| resting stage, not cycling, nutrients scarce |
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Term
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Definition
| Preparation for synthesis, cellular metabolism |
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Term
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Definition
| DNA and Histone Synthesis |
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Term
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Definition
Early-replicating DNA
G-light (negative) bands |
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Term
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Definition
Rest period
Period of cellular inactivity between synthesis of early and alte replicating DNA at which time approximately three complements of DNA are present in the cell. |
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Term
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Definition
Late-replicating DNA
G-dark (positive) bands. |
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Term
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Definition
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Term
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Definition
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Term
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Definition
| DNA begins to condense; nuclear membrand dissolves |
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Term
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Definition
| Maximum condensation; chromosomes line up (cytogeneticists collect cells at this phase for analysis) |
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Term
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Definition
| Movement to poles; constriction |
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Term
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Definition
| Membranes reassembled; cell migration |
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Term
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Definition
Culture vessel open to the environment (free exchange of gases between air inside culture vessel and surrounding air inside incubator). Dishes and flasks with loose caps; vented flasks.
Requirements: 5% CO2 in a moisture saturated air to interact with the bicarbonate buffer of media to maintain a media pH of 7.2-7.4 (optimum). |
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Term
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Definition
| Enzyme that digests collagen. |
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Term
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Definition
| Enzymes that digest protein. |
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Term
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Definition
| Serum additive that inactivates trypsin |
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Term
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Definition
| Solution of inorganic salts compatible with the cell's salt composition (isotonic). Used for making basal media. |
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Term
| What tissue types require removal of maternal decidua? |
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Definition
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Term
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Definition
| Contains minimum essential ingredients to support cellular growth |
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Term
| Modified or Enriched Media |
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Definition
| Basal media plus amino acids, vitamins, minerals, etc. for maximum growth of a particular cell type (RPMI 1650, Mccoy's, etc.) |
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Term
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Definition
| Media with only characterized components (i.e., specific growth hormones to enhance growth of typical amniocytes) |
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Term
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Definition
| Media containing all required nutrients and supplements plus serum at a high percentage. NOTE: All media becomes complete with addition of serum. Used to promote active cellular growth. |
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Term
| Maintenance or Shipping Media |
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Definition
| Complete media with a decreased percentage of serum. Used when active growth should not be encouraged |
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Term
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Definition
| Complete media with DMSO or glycerol. Used for freezing cells. |
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Term
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Definition
| Media exposed to cycling cells producing small molecular metabolites and macromolecules. Secreted molecules act as growth hormones to the new cells being cultured. |
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Term
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Definition
| Supplement in media that produces sodium carbonate, water, and carbon dioxide. |
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Term
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Definition
| Used as an energy source for metabolism in media. |
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Term
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Definition
| Usually fetal bovine serum, fetal calf serm, etc. Provides sugars, proteins and trace nutritional elements. |
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Term
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Definition
| Essential amino acid added as a supplement in media that cannot be manufactured by a cell's components. |
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Term
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Definition
| Mitogen commonly used for blood culturing. Time and temperature dependent. |
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Term
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Definition
| Added to solutions to visibly monitor pH. |
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Term
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Definition
| Additive used to kill or inhibit bacteria. |
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Term
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Definition
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Term
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Definition
| Normal component of media; co-factors for enzyme function. |
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Term
| Steps to check media sterility |
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Definition
Aliqot basal media into sterile vessel.
Incubate for 3 days and check sterility by inspecting for turbidity and pH shift. |
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Term
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Definition
| Direct or overnight up to 96 hours |
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Term
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Definition
Usually more than 5 cell cycles.
Primary cultures from solid tissues are long term cultures.
Amniotic fluid: 5-10 days
CVS: 5-8 days
Solid tissues (non-cancerous): 5-12 days
Solid tumors: 1-20 days |
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Term
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Definition
| Once a culture is subcultured it becomes a cell line. Can be finite or continuous. |
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Term
| Guidelines for appropriate labeling technique. |
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Definition
Unique lab ID number
Patient name
Culture identificiation |
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Term
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Definition
Deplete media of nutrients.
pH shift to acid (yelow) which causes cell cycling to stop.
Massiv contamination prdodu |
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