Term
Which nucleotide pair has high frequency of mutation in human chromozomal DNA? |
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Definition
CG
Spontaneous deaminazation of methylcytosine results in thymine formation; it occurs 2.5 X more frequently than any other single nucleotide mutation |
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Term
| During the 2 phase of the cell cycle, the DNA content of a cell is |
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Definition
Tetraploid
The G2 phase of the cell cycle follows the S phase, where NA content goes from 2N to 4N or from diploid to tetraploid |
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Term
| Intervening -sequences are found in |
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Definition
Hetero-nuclear RNA
Hetero-nuclear RNA is made up of newly transcribed mRNA that still contains intervening sequences. |
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Term
| Which is considered an epigenitic modification of DNA |
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Definition
Methylazaton of CpG islands in the promoter region of a gene.
Methylazation of CpG islands is an epigenetic mechanism of gene silencing. |
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Term
| A metaphase chromozome with primary constriction that gives the chromozome clearly defined short and long arms is considered |
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Definition
Sub-metacentric
Chromosomes with clearly defined short and long arms are known as submetricentric chromosomes |
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Term
| Reverse transcriptase would best be described as a: |
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Definition
RNA dependent DNA polymerase
RT synthesizes a DNA molecule from an RNA template |
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Term
The 2 allels for a sex-linked resessive disease are X and x. The mutant alleal is x. What is the percentage of male offspring that would be expected to be affected by the disease from from parents who have the following genotypes?
mother Xx
Father xY
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Definition
50%
One X chromosome is passed on to all sons
by the mother; since she is a carrier of a mutant X alleal, 50% of her sons will be affected. |
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Term
| The term that best describes males reguarding the X-linked genes is: |
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Definition
Hemizygous
Males are hemizygous for X linked allels because they have only one copy of the X chromozome. |
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Term
| The mode of inheritance of mitochondrial DNA is: |
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Definition
Maternal
All mitochondra are passed on from a mother to her children, but not by a father to his children |
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Term
| Which statement concerning the resoluton of DNA on agarose gel is true |
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Definition
As the concentration of agarose in the gel increases, the smaller the size of DNA that can be resolved on the gel.
The greater the concentration of agarose, the smaller the size of DNA that can be resolved. |
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Term
| An RFLP is most likely detected using which of the following methods? |
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Definition
Southern blot
RFLP analysis is preformed on DNA. Southern blotting id the method used to separate DNA fragments bby size, transfer the DNA fragments to a solid support, and then hybridize with a probe specific for the gene of interest, |
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Term
| The methodology of performing a reverse dot blot is described as: |
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Definition
| Attaching multiple probes to a nitrocellulose membrane and then hybridizing a singlelabeled patient sample to multiple probes |
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Term
| Which condition has the higest stringency for washing a southern blot after hybridization has been completed. |
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Definition
| High temperature ,low salt concentration |
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Term
| The Klenow fragment of E-coli DNA polymerasse 1 is used |
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Definition
| To label DNA probes by random primer lableing |
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Term
| What is a solution hybridization method for the detection of nucleic acid protein interaction? |
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Definition
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Term
| Which probe is most often used to detect trisomy 21 by interphase fish? |
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Definition
| Alpha satelitte DNA probe |
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Term
| How many volumes of ethanol are added to 1 volume of a DNA salt solution to cause the DNA to precipitate? |
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Definition
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Term
| Chelex 100 resin is used to: |
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Definition
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Term
| The fluorescent dye that exhibits the greatest sensitivity for quantitation of DNA is: |
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Definition
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Term
| When quantifying the amount of genomic DNA in a sample by spectrophotometry, an OD 260 of 1.0 cooresponds to what concentration of DNA |
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Definition
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Term
| An RNA sample is isolated from pheriheral blood cells of a patient. When performing spectrophotometry analysis to determine the yeald of RNA in the sample you find the 1:40 dilution of the 0.5 mL sample gives an OD 260 reading of 0.03125 and an OD 280 reading of 0.01760. What is the total amount of RNA contained in the 0.5mL sample? |
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Definition
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