Term
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Definition
| partially original protein and part protein from inserted gene |
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Term
|
Definition
| inserted gene for somatostatin into E. coli to produce large quanities |
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Term
|
Definition
| first recombinant DNA product of medical value |
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Term
|
Definition
| mutation in adenosine deaminase gene |
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Term
|
Definition
| treated SCID disease w/ gene therapy |
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Term
|
Definition
| connected with herbicide resistance |
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Term
| bacillus thuringensis toxin |
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Definition
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Term
| Methods to insert genes into plant cells |
|
Definition
1. tungstsen/gold bullet
2. chemical treatment
3. microinjection
4. electroporation
5. virus |
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Term
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Definition
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Term
|
Definition
| contains Ti plasmid and causes crown gall disease |
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Term
|
Definition
| kandamycin and spectromycin resistance genes |
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Term
|
Definition
insert genes into bacteria.
grow bacteria on spectinomycin infused culture to select for those that took up vector
infuse into plant cells
test with kandamycin |
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Term
|
Definition
| bioluminexcent enzyme found in fireflies that is often used as a reporter gene |
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Term
|
Definition
| produces a similar gene to luciferin (lux operon) |
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Term
| green fluorescent protein |
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Definition
| found in jellyfish; often used to label proteins |
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Term
| Restriction Mapping viruses process |
|
Definition
1. extract DNA
2. subject to rest. enzymes
3. agarose gel electrophoresis |
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Term
| Which process is used to establish relationship between organisms? |
|
Definition
| Restriction mapping (RFLPs) |
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Term
| __________ can be used to associate a geno or phenotype with a region of a chromosome |
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Definition
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Term
| Pre-natal diagnosis procedure |
|
Definition
1. extract cells via amniocentesis
2. extract DNA
3. incubate w/ MstII
4. agarose gel electro
5. Southern blot |
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Term
|
Definition
| enzyme which cleaves normal hemoglobin, but not SCA hemoglobin |
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Term
|
Definition
| point mutation in Beta subunit of hemoglobin |
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Term
|
Definition
1. Restriction digest
2. subject digest to agarose gel electro
3. denature DNA w/ alkaline solution
4. Set up blot |
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Term
| How to set up a southern blot |
|
Definition
1. place gel on platform over buffer
2. drape filter paper over platform
3. lay nylon membrane over gel
4. cover w/ several layers of filter paper
5. incubate nylon membrane w/ radioactive probe |
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Term
|
Definition
|
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Term
|
Definition
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Term
|
Definition
| southern blot w/o electrophoresis |
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Term
| Probes can be made of ___________ or ___________. |
|
Definition
| gene products (proteins) or amino acid sequences |
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Term
|
Definition
| short pieces of DNA (PCR primers) |
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Term
|
Definition
| similar sequence in closely related species |
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Term
|
Definition
| Avidin w/ peroxidase has a strong attraction to biotin |
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Term
|
Definition
| microchip w/ ssDNA oligonucleotide |
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Term
|
Definition
| allow only exact hybridization |
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Term
|
Definition
| used RFLPs from known chromosomes as a probe to isolate gene product (Huntingen) |
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Term
|
Definition
| collected samples from people with Huntington's disease |
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Term
|
Definition
|
|
Term
| Chromosome of Huntington's Disease |
|
Definition
|
|
Term
| Chromosome of Alzheimer's |
|
Definition
|
|
Term
| Chromosome of Duchenne's MD |
|
Definition
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|
Term
| Chromosome of Lou Gehrig's disease |
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Definition
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|
Term
| Dideoxy sequencing procedure |
|
Definition
1. clone ss rest fragments
2. mix cloned DNA w/ primer
3. in vitro DNA synth w/ Klenow fragment
4. add 4 normal nucleoside triphosphates in excess and analog of one in limited amount |
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Term
|
Definition
| DNA polymerase I w/o exonuclease ability |
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Term
|
Definition
| method of sequencing DNA by using overlapping ends |
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Term
|
Definition
| developed site directed mutagenesis |
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Term
| Site directed mutagenesis procedure |
|
Definition
1. insert known sequence into viral ssDNA
2. use primer w/ base subst.
3. insert into bacteria and allow to replicate
4. Replication leads to mutants and wild types |
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Term
|
Definition
| site directed mutagenesis or knockout genetics |
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Term
|
Definition
low resolution recombination data using:
polymorphic markers
phenotypic variations
RFLPs
variation in tandem repeats. |
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Term
|
Definition
| low resolution physical mapping that associates a gene with a chromosome band |
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Term
|
Definition
| fluorescence in sito hybridization |
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Term
|
Definition
| complimentary DNA; used as probe for expressed sequences, since it is derived from mRNA |
|
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Term
|
Definition
Francis Collins; top down mapping
uses rare cutters and overlapping ends to sequence |
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Term
|
Definition
Craig Venter; bottom up mapping
cloning of fragments and overlapping to sequence |
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Term
|
Definition
| restriction enzymes that cut only every 1000-1 million base pair |
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Term
|
Definition
| tie genetic disease to specific sequence in humans and use model organisms to experiment |
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Term
|
Definition
| isolation of exons of expressed genes |
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Term
|
Definition
|
|
Term
| DNA Fingerprinting was developed by _____. |
|
Definition
|
|
Term
|
Definition
| detect repetitive DNA loci throughout genome and generate a pattern of 20-30 bands |
|
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Term
|
Definition
| variable number tangent repeats |
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|
Term
|
Definition
| single repetitive locus generates 1 or 2 bands per individual (depends whether it is homozygous or not) |
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|
Term
| Probability of unrelated individual having the same bands with 1 SLP (1-2 bands) |
|
Definition
|
|
Term
| Probability of unrelated individual having the same bands with 2 SLPs (2-4 bands) |
|
Definition
|
|
Term
| Probability of unrelated individual having the same bands with 4 SLPs (<8 bands) |
|
Definition
|
|
Term
| Probability of unrelated individual having the same bands with MLP (20-30 bands) |
|
Definition
|
|
Term
|
Definition
combined DNA Index System
database of DNA tests from judicial systems |
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