Term
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Definition
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Term
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Definition
| Thymine, Cytosine and Uracil |
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Term
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Definition
| http://hal.weihenstephan.de/genglos/images/adenine.gif |
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Term
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Definition
| http://upload.wikimedia.org/wikipedia/commons/c/cf/Guanine_chemical_structure_2.png |
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Term
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Definition
| http://www.molecularstation.com/molecular-biology-images/data/502/thymine-chemical-structure.png |
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Term
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Definition
| http://upload.wikimedia.org/wikipedia/commons/thumb/8/8b/Cytosine_chemical_structure.svg/301px-Cytosine_chemical_structure.svg.png |
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Term
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Definition
| http://images2.wikia.nocookie.net/__cb20071105003559/psychology/images/9/9e/Uracil_chemical_structure.png |
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Term
| The nitrogenous base ______ in RNA is altered to produce ________. |
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Definition
| uracil, thymine (5-methyluracil) |
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Term
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Definition
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Term
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Definition
| nitrogenous base + sugar + phosphate |
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Term
| This nucleoside has a ribose rather than deoxyribose understood in its name |
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Definition
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Term
| Another name for adenylate is ______. |
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Definition
| adenylic acid or adenosine monophosphate |
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Term
| H. sapiens have a DNA of ___________ Mb. |
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Definition
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Term
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Definition
| determined proportions of bases varied amongst species, but always A=T and G=C |
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Term
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Definition
| used x-ray diffraction analysis to determine that DNA had a spiral configuration and complementarity w/in the structure |
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Term
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Definition
| concluded that DNA is a double helix with bases of antiparallel chains pairing via H-bonds |
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Term
| Watson & Crick published their findings in ______________. |
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Definition
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Term
| Semi-conservative model of DNA replication |
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Definition
| proposed by Watson & Crick, each helix composed of one original and one entirely new strand |
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Term
| Conservative Model of DNA Replication |
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Definition
| newly synthesized and original helices are separate |
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Term
| Dispersive model of DNA replication |
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Definition
| new and original bases are interspersed throughout each strand |
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Term
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Definition
grew labelled DNA on unlabelled medium, then centrifuged the DNA. Yielded hybrid DNA and labelled DNA.
Ruled out conservative model.
Repeated experiment to yield half hybrid and half unlabelled.
Ruled out dispersive model. |
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Term
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Definition
| isolated DNA polymerase from E. Coli and combined it with equal amts of 4 nucleosides and primer |
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Term
| True or False: 2 DNA strands which are complementary will have the same base pair ratio, but not not necessarily the same purine/pyrimidine ratio |
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Definition
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Term
| True or False: A ssDNA will have the same base pair ratio as its double helix and will also have the same purine/pyrimidine ratio |
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Definition
| False; the PP ratio may differ |
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Term
| True or False: 2 identical strands of DNA will have the same base pair and purine/pyrimidine ratios |
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Definition
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Term
| Nearest Neighbor Analysis Steps |
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Definition
1. Label deoxyribonucleosides w/ 32P
2. Use each in an in vitro DNA synth
3. Treat product DNA w/ spleen diesterase
4. Determine frequency of labelled phosphates transfer to a nucleotide
Corroborated complementarity and opposite polarity of strands |
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Term
| Classifications of Nucleases |
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Definition
1. By Substrate
2. By ester bond
3. BY manner of attack |
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Term
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Definition
| attacks only free end of DNA chain |
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Term
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Definition
| cleaves interior bonds of DNA chain |
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Term
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Definition
| cleave at the 3' end of the ester bond |
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Term
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Definition
1. Label E. Coli w/ tritiated thymidine
2. Extract DNA
3. Cover w/ photographic emulsion
4. Analyze radiographs
Confirmed replication fork |
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Term
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Definition
1. Label E. Coli w/ tritiated thymidine
2. Submit to brief pulse of radioactivity
3. Isolate and denature DNA
4. Subject to density gradient centrifugation
Discovered that DNA assembled in short gragments |
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Term
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Definition
| links preformed strands of DNA; follows polymerase I to attach replacement strands |
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Term
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Definition
| removal & replacement of RNA primers from growing DNA strand |
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Term
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Definition
| involved in helical repair |
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Term
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Definition
| primary replicating enzyme responsible for chain extension and proofreading |
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Term
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Definition
| produces short strand of primer RNA using DNA as template |
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Term
| Initiator protein (dnaA protein) |
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Definition
| binds at beginning of replication site |
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Term
| DNA helicase (dnaB protein) |
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Definition
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Term
| DNA Helicase (reg proteins) |
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Definition
| force strands apart at replication fork |
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Term
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Definition
| removes kinks or supercoils for one strand |
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Term
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Definition
| removes kinks or supercoils from both strands |
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