Term
| What is the % difference between individual humans that is used to distinguish them? |
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Definition
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Term
True or False:
Blood Typing is more useful for exclusion of potential suspects |
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Definition
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Term
| Certain regions in our genome have DNA sequences that repeat over and over again, what are these repeating units known as? |
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Definition
| Variable Number Tandem Repeats |
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Term
| What do you use to digest DNA and "cut out" VNTRs? |
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Definition
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Term
| What technique do you use to analyze VNTRs? |
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Definition
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Term
| Define DNA Fingerprinting |
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Definition
| An individual's unique banding pattern |
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Term
| VNTR sequences are how many nucleotides long? |
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Definition
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Term
| How many times are the VNTR sequences repeated head to tail (in tandem)? |
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Definition
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Term
| What are VNTRs with respect to the number of repeats? |
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Definition
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Term
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Definition
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Term
| If a particular VNTR repeat is single locus where does it occur? |
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Definition
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Term
| If a particular VNTR repeat is Multi-locus where does it occur? |
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Definition
| Occurs in multiple places |
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Term
| Where is the locus generally at? |
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Definition
Non-coding regions
-introns & between genes (extragenic) |
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Term
True or False:
The function of tandem repeats is unknown |
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Definition
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Term
What are the following synonyms for:
Minisatellites
Minisatellite DNA |
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Definition
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Term
What are the following synonyms for:
Microsatellites
Microsatellite DNA |
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Definition
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Term
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Definition
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Term
True or False:
VNTRs are difficult to analyze
Require moderate amounts of INTACT DNA |
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Definition
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Term
True or False:
With Forensic Cases the VNTRs are sometimes degraded and the amount is often small |
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Definition
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Term
| What is the time period to analyze VNTRs? |
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Definition
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Term
| STR sequences are how many nucleotides long? |
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Definition
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Term
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Definition
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Term
| Can PCR be used with STR? |
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Definition
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Term
True or False:
Only a few copies of INTACT DNA are needed in degraded specimen with STR |
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Definition
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Term
| What is the time range to analyze STRs? |
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Definition
| Can be automated and performed in HOURS |
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Term
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Definition
| Federally maintained database of DNA obtained from crime scenes and convicted violent offenders |
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Term
| What does CODIS stand for? |
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Definition
| Combined DNA Index System |
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Term
| CODIS uses how many STR Loci on various chromosomes amplified by PCR? |
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Definition
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Term
True or False:
CODIS also analyzes amelogenin locus (determines gender) |
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Definition
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|
Term
True or False:
X & Y chromosome have different size PCR products |
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Definition
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Term
| What does an Amelogenin locus determine? |
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Definition
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Term
| PCR Products are separated by _____ by polyacrylamide gel electrophoresis |
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Definition
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Term
| What analyzes size and fluorescence of DNA STRs? |
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Definition
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Term
|
Definition
Forensic cases
Paternity testing
Monitoring BM Engraftment |
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Term
| What is the Hardy-Weinberg equation? |
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Definition
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Term
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Definition
| Frequency of the appearance of A allele |
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Term
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Definition
| Frequency of the appearance of a allele |
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Term
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Definition
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Term
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Definition
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Term
| What is the locus frequency calculation for a heterozygous pair? |
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Definition
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Term
| What is the locus frequency calculation for a homozygous pair? |
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Definition
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Term
True or False:
Improper collection of crime scene specimen may invalidate test results |
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Definition
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|
Term
True or False:
1 hair can implicate someone for a crime |
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Definition
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|
Term
| What does sneezing and coughing do? |
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Definition
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Term
| Mitochondrial DNA is ONLY what type of inheritance? |
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Definition
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Term
True or False:
mtDNA is non-nuclear and single-stranded DNA |
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Definition
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|
Term
True or False:
mtDNA is non-nuclear and double-stranded DNA |
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Definition
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Term
| What are mtDNA genes involved in? |
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Definition
| Kreb's cycle for energy production |
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Term
True or False:
mtDNA is MORE stable than chromosomal DNA |
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Definition
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|
Term
| What is the copy number of mtDNA? |
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Definition
|
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Term
True or False:
mtDNA Hypervariable region which varies between individuals |
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Definition
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|
Term
| What 2 things is mtDNA useful for? |
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Definition
Identifying individuals in disasters
"Cold Cases" |
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Term
| Y Chromosome analysis can only occur in what population? |
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Definition
| Males; Inherited from father |
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Term
| What are the 3 factors to be considered when comparing tests? |
|
Definition
Speed
Cost effectiveness
Power of Discrimination |
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|
Term
| What does SNPs stand for? |
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Definition
| Single Nucleotide Polymorphisms |
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|
Term
| What does AFLP stand for? |
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Definition
| Amplified fragment length polymorphisms |
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|
Term
True or False:
SSNPs don't affect gene product or gene regulation |
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Definition
|
|
Term
| What does SSNP stand for? |
|
Definition
| Silent single nucleotide polymorphisms |
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|
Term
| How many markers are associated with SNPs? |
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Definition
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Term
|
Definition
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|
Term
| In AFLP what is DNA treated with? |
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Definition
| Restriction enzymes; Specific sequences amplified |
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Term
| Which is more costly SNPs or STR analysis? |
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Definition
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|
Term
| Which type of technique involves single base pair polymorphisms? |
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Definition
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Term
| What has a HIGH Power of Discrimination and a SLOW speed of Analysis? |
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Definition
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Term
| What has a HIGH Power of Discrimination and a FAST speed of Analysis? |
|
Definition
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Term
| What has a LOW Power of Discrimination and a SLOW speed of Analysis? |
|
Definition
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Term
| What has a LOW Power of Discrimination and a FAST speed of Analysis? |
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Definition
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Term
| STRs used in forensics are highly polymorphic. How many different alleles are there detected for each locus? |
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Definition
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|
Term
| The polyacrylamide gel used to separate amplified DNA fragments can distinguish alleles that differ in how many nucleotides? |
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Definition
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|
Term
| Jeffreys DNA fingerprinting method is known as what? |
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Definition
| Multi-locus fingerprinting (VNTR) |
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