Term
| Name four things a frozen section may be used for? |
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Definition
Tissue is examined while the patient is in the operating room to determine:
• Presence or absence of malignancy
• Presence or absence of inflammation/organisms
• Whether surgical margins are free of neoplasm
• Whether diagnostic tissue is present |
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Term
| Use monoclonal antibodies to identify cell products or surface markers |
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Definition
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Term
| intermediate filaments Keratins |
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Definition
| Carcinomas, mesotheliomas |
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Term
| intermediate filaments Desmin |
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Definition
| Muscle tumors: smooth, striated |
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Term
| intermediate filament Vimentin |
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Definition
| Mesenchymal tumors, some carcinomas |
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Term
| intermediate filaments• Glial filaments |
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Definition
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Term
| intermediate filament Neurofilaments |
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Definition
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Term
| What two things can flow cytometry measure? |
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Definition
| cell characteristics such as membrane antigens and DNA content of tumor cells |
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Term
| Can analyze 5000 to 10,000 cells per second |
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Definition
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Term
| Suspension of cells flows through beam of light past stationary detectors |
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Definition
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Term
| Uses Interphase cytogenetics |
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Definition
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Term
| What advantage does FISH have over standard karyotyping? |
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Definition
| Analysis of chromosomes in nondividing cells |
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Term
Uses DNA probes which recognize
chromosome specific sequences |
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Definition
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Term
| –Demonstrate subtle microdeletions and complex translocations |
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Definition
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Term
| Most frequently used molecular technique in molecular pathology laboratory |
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Definition
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Term
– Millions of copies of DNA sequences can be generated within few hours
– Can also amplify RNA |
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Definition
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Term
| What six areas are genomic approaches having an impact on? |
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Definition
Tumor classification
Prognostic markers
Predictive indicators of drug response
The development of new drug therapies
Strategies for monitoring disease
Management of susceptibility to cancer |
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Term
| What does a DNA microarray analysis measure? |
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Definition
| Can simultaneously measure expression levels of several thousand genes |
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Term
– Essentially gives a molecular profile for each tissue analyzed
– Uses gene chip technology |
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Definition
| • DNA microarray analysis |
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