Term
| Do Restriction Endonucleases cleave both DNA strands? |
|
Definition
|
|
Term
| Which enzyme joins DNA fragments together by reforming the sugar-phosphate bonds of DNA? |
|
Definition
|
|
Term
| A foreign DNA fragments was first inserted into a ? |
|
Definition
|
|
Term
| What type of "ends" are produced by EcoRI? |
|
Definition
|
|
Term
| What is the name of a DNA carrier? |
|
Definition
|
|
Term
| What is the name of the Process used to transfer DNA into bacteria? |
|
Definition
|
|
Term
| What normally repels DNA from cell membranes? |
|
Definition
Negatively-charged phosphate in lipid molecules
OR
Negatively charged phosphates on DNA helix repelled by lipids |
|
|
Term
| What is the purpose of the calcium used in this method? |
|
Definition
|
|
Term
| Why is the temperature lowered in the RE Project? |
|
Definition
| Congeals lipid membrane & stabilizes the negatively charged phosphates |
|
|
Term
| What happens when there is a rapid rise in the temperature? |
|
Definition
| A temperature imbalance is created and DNA is swept into the cell through an adhesion zone |
|
|
Term
| What are the five different types of DNA libraries? |
|
Definition
*Yeast artificial chromosomes
*Bacterial Artificial Chromosomes
*Cosmids
*Bacteriophage lambda
*Plasmids |
|
|
Term
| Which of the 5 libraries was the workhorse for the human genome project? |
|
Definition
| Bacterial Artificial Chromosomes |
|
|
Term
| What 6 items are needed to make the Agarose Electrophoresis gel? |
|
Definition
Agarose
Buffer
Flask
Microwave
Gel mold
Gel comb |
|
|
Term
| How long does it usually take for a gel to solidify? |
|
Definition
|
|
Term
| What is the purpose of the comb? |
|
Definition
|
|
Term
| What type of pipette is used to transfer the DNA sample into a well in the gel? |
|
Definition
|
|
Term
| Electrophoresis - Does the black cord generate a positive or negative charge? |
|
Definition
|
|
Term
| Electrophoresis - Does the red cord generate a positive or negative charge? |
|
Definition
|
|
Term
| When examining the gel box, what tells you that the current is running? |
|
Definition
|
|
Term
| Name the scientist who developed the 1st DNA sequencing technique |
|
Definition
|
|
Term
| The # of readable base pairs in a typical sequencing reaction is what? |
|
Definition
|
|
Term
| What is the name of the computer "output" which shows the colored peaks representing each nucleotide in a DNA sequence? |
|
Definition
|
|
Term
| Most clone libraries store fragment DNA in which bacterium? |
|
Definition
|
|
Term
| A single sequencing reaction can reveal the order of how many DNA bases? |
|
Definition
|
|
Term
| On average, how many times is each base pair sequenced? |
|
Definition
|
|
Term
| How many sequencing reactions were done in the human genome project? |
|
Definition
|
|
Term
| The accuracy of the finished genome sequence is: |
|
Definition
|
|
Term
| How many disease genes were identified by the project's conclusion: |
|
Definition
|
|
Term
| What is used to detect the DNA fragments as they migrate to the bottom of the gel? |
|
Definition
|
|
Term
| Did the public and private sectors work together on the Human Genome Project? |
|
Definition
| No. There was a major feud between the two sectors |
|
|
Term
| Did the public or private sector first use automated DNA sequencers to map the human genome? |
|
Definition
|
|
Term
| Which U.S. president sent a mediator to end the feud between the public and private sectors working on mapping the genome? |
|
Definition
|
|
Term
| What year was the first draft of the human genome announced? |
|
Definition
|
|
