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Molecular Biology of the Gene
n/a
53
Biology
Undergraduate 1
10/19/2011

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Term
what is a gene?
Definition
- discrete units of inheritance (segments of DNA)
Term
what does a gene consist of?
Definition
- specific sequence of nucleotides in DNA
Term
what is a chromosome?
Definition
- a single piece of coiled DNA containing many genes
Term
what is DNA?
Definition
- a double strand polymer of nucleotides
Term
3 main structures of DNA
Definition
- phosphate
- 5 carbon sugar (deyoxribose)
- nitrogen base
Term
what are the 4 different nitrogen bases of DNA?
Definition
- Adenine
- Guanine
- Thymine
- Cytosine
Term
Of the 4 nitrogen bases of DNA, which 2 are double ringed purines?
Definition
Adenine and Guanine
Term
of the 4 nitrogen bases of DNA, which 2 are single ringed pyrimidines
Definition
thymine and cytosine
Term
the phosphate and 5 carbon sugar combine to form what?
Definition
phosphate-sugar complex
Term
how are phosphate-sugar complexes bonded together?
Definition
covalent bonds
Term
how are the nitrogen bases bonded to the phosphate-sugar complexes?
Definition
covalent bonds
Term
How are the double strands of DNA bonded together?
Definition
hydrogen bonds via the nitrogen bases
Term
In DNA what nitrogen base always pairs with Adenine?
Definition
Thymine
Term
In DNA what nitrogen base always pairs with Cytosine?
Definition
Guanine
Term
In DNA what is the name for three successive bases on a DNA strand?
Definition
triplet
Term
Where does DNA replication take place?
Definition
in the nucleus
Term
Why does DNA replicate?
Definition
- to ensure continuity of hereditary traits
Term
What is semi-conservative DNA replication?
Definition
- conserves some of the old strand with the new strand
Term
Where does DNA unwind?
Definition
origin of replication
Term
What 2 enzymes cause the DNA to separate?
Definition
- helicase
- DNA nuclease
Term
How do helicase and DNA nuclease separate the DNA strands?
Definition
they break the hydrogen bonds
Term
In DNA replication what is the exposed strand called?
Definition
template strand
Term
In DNA replication what enzyme joins the new nucleotides to the template strand?
Definition
DNA polymerase
Term
In DNA replication what is formed when the new nucleotides join with the template strand?
Definition
complimentary strand
Term
What enzyme connects the new segments of DNA?
Definition
Ligase
Term
What is RNA
Definition
single strand polymer of nucleotides
Term
What are the 3 structures of RNA?
Definition
- phosphate
- 5 carbon sugar (ribose)
- nitrogen base
Term
What are the 4 possible nitrogen bases for RNA?
Definition
- Adenine
- Cytosine
- Guanine
- Uracil
Term
What are the 3 differences between DNA and RNA?
Definition
- different sugars
- DNA double strand/RNA single strand
- DNA = thymine / RNA = uracil
Term
What are the 3 types of RNA involved in protein synthesis?
Definition
- messenger RNA (mRNA)
- transfer RNA (tRNA)
- ribosomal RNA (rRNA)
Term
what is the function of mRNA?
Definition
- carries code from DNA to ribosomes
Term
what is the function of tRNA?
Definition
carries amino acids to mRNA at ribosomes
Term
what is the function of rRNA?
Definition
responsible for peptide bond
Term
What is the name for 3 successive bases on mRNA
Definition
codon
Term
What is the name for 3 successive bases on tRNA
Definition
anticodon
Term
What happens in RNA transcription?
Definition
DNA information into a molecule of RNA
Term
What happens in RNA translation?
Definition
RNA to protein (or polypeptite chain)
Term
Where does RNA translation occur?
Definition
in the cytoplasm/ribosome
Term
Where does the RNA strand go after transcription?
Definition
out into the cytoplasm
Term
What are the 3 main steps of RNA translation?
Definition
- initiation
- polypeptide formation (elongation)
- termination
Term
In the initiation of RNA translation, what does mRNA attach to?
Definition
ribosomal subunit
Term
in the initiation of RNA translation, what does tRNA attach to?
Definition
codon of mRNA
Term
In RNA elongation, a polypeptide bond is formed between what?
Definition
two amino acids
Term
What causes the termination of RNA translation?
Definition
stop codon
Term
What do ribosomes use to translate bases into amino acids?
Definition
use tRNA to attach to mRNA
Term
What is a DNA mutation?
Definition
a heritable change in the DNA
Term
what does heritable mean (in terms of DNA mutations)?
Definition
it can move from one line (generation) of cells to the next line of cells
Term
What are 3 common causes of DNA mutations?
Definition
- radiation
- viruses
- mutagenic chemicals
Term
T or F
The information from mutated DNA is NOT likely to be passed on to RNA
Definition
FALSE
Term
what are the 2 major types of DNA mutation?
Definition
- point mutations
- frameshift mutations
Term
what is a point mutation?
Definition
a substitution of a base
Term
what is a frameshift mutation?
Definition
a deletion or insertion of a base
Term
what is a silent mutation?
Definition
when a change would not affect the amino acid produced
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