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Definition
| Combination of plasmid vector and COS site that allows target DNA to be inserted into the 1 head. Has high transformation efficiency, can carry up to 45kb plasmid |
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| Coding region for a gene may be interrupted by introns, non-coding regions included, unbiased representation of expression levels |
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| Introns are removed, no junk DNA, biased representation of coding sequence expression levels |
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| Nicks the RNA strand of the RNA/DNA hybrids, possesses 5-3/3-5 exoribonuclease activity. Nicks RNA and digests in both directions |
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| Extends the RNA primer to synthesize second strand cDNA. Has 5-3 exonuclease activity that removes RNA fragments in front of enzyme. |
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Definition
| Links fragments created by E.coli DNA Pol I, adds synthetic oligos that anneal to create a cut restriction enzyme site for cloning. |
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Definition
| 5-3 DNA pol, 3-5 exonuclease, used for creating blunt ends of DNA with 5 or 3 overhangs |
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Definition
| Oligos created by the addition of T4 DNA ligase |
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| Terminal deoxynucleotidyl transferase (TdT) |
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Definition
| Used to add a homopolymetric tail to the 5' strand of the first cDNA strand |
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Definition
| Every fragment is part of the screen, screen is hybridization-based |
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Definition
| Every fragment is part of a function-based screen, frame, orientation and genomic position considerations limit the proportion of the library that can be usefully screened |
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Term
| Nucleic Acid Hybridization |
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Definition
| Occurs in replication, transcription, translation (rRNA/tRNA structures), recombination, RNA interference, used to study gene structure and function |
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Definition
| Used to identify a gene from a non-expression library, is a nucleic acid that is complementary to a specific gene or nucleic acid sequence of interest. |
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Definition
| Sequence is exactly complementary to the nucleic acid sequence you are searching for. |
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| Heterologous (Degenerate) Sequence |
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Definition
| Sequence is similar to, but not exactly complementary. |
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