Term
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Definition
| Enzyme activity / Total protein concentration |
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Term
| Michaelis-Menton kinetics assumes: |
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Definition
Only 1 substrate
Substrate concentration is not limiting
Only the initial rate is considered |
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Term
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Definition
| Vmax x (Substrate concentration/(Km + substrate concentration) |
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Term
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Definition
| Number reactions per second |
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Term
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Definition
| Cataltic speed vs binding affinity |
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Term
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Definition
| How long without enzyme vs how long with enzyme |
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Term
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Definition
| Determined that the proteins structure was held in the primary structure, not the cysteine bridges |
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Term
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Definition
| Inactive precursor to a protein |
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Term
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Definition
| C end of positive residue |
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Term
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Definition
| C end of hydrophobic resisdue |
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Term
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Definition
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Term
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Definition
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Term
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Definition
| Coat wells in protein to determine purity |
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Term
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Definition
| Electrophoresis stained with anti-body and colour chageing enzyme to determine purity |
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Term
| Density gradient centrifugation speed |
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Definition
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Term
| When and who discovered DNA |
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Definition
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Term
|
Definition
| Smooth virus kills but rough dosesn't, dead smooth and rought does |
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Term
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Definition
| If you remove DNA the virus no longer kills |
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Term
|
Definition
| DNA is exchanged when virus infects, not protien |
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Term
|
Definition
| Developed conservative model |
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Term
| Neucleotide used in DNA sequencing to terminate chain |
|
Definition
| Dideoxyneucleotide triphosphate |
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Term
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Definition
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Term
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Definition
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Term
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Definition
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Term
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Definition
| 36 places after TTGACA and 10 places after AT rich region |
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Term
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Definition
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Term
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Definition
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Term
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Definition
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Term
| Prokaryotic RNA binding site |
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Definition
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Term
| Eukaryotic ribosome biding site |
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Definition
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Term
| Temperature of incubation for creation of DNA vector |
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Definition
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Term
| Prokaryotic promotor sequence |
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Definition
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|
Term
| Eukaryotic promotor sequence |
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Definition
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Term
| Temperature used to denature in PCR |
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Definition
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Term
| Temperature used to anneal in PCR |
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Definition
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Term
| Temperature for DNA polymerase to work in PCR |
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Definition
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Term
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Definition
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Definition
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Definition
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