Term
| Where does the Or operator lie? |
|
Definition
| between c1 (lambda repressor) and cro |
|
|
Term
| What three sites are contained in the Or operator? |
|
Definition
|
|
Term
|
Definition
| promotes the transcription of lytic genes |
|
|
Term
|
Definition
| directs the transcription of th cI gene |
|
|
Term
| Over which two opposing promoters does the Or overlap? |
|
Definition
|
|
Term
| Are the three operator sites of the operator region of the phage lambda genome identical? |
|
Definition
| No, they are similar but not identical. |
|
|
Term
| Where does lambda repressor bind on the Or region? |
|
Definition
| Lambda repressor binds to the Or1 region with the highest affinity |
|
|
Term
| Where does cro bind on the OR region? |
|
Definition
| Cro binds to OR3 with the highest affinity |
|
|
Term
| What happens when lambda repressor binds to Or1? |
|
Definition
| It blocks the transcription of the genes for the lytic cycle because it blocks transcription from Pr |
|
|
Term
| What happens when cro occupies OR3? |
|
Definition
| It blocks transcription from Prm which blocks maintenance of CI transcription and therefore allows the transcription of genes for the lytic cycle. |
|
|
Term
| This determines the lytic-vs.- lysogenic patterns of the lambda gene expression |
|
Definition
| The occupation of the repressor sites |
|
|
Term
| Is a established lysogen stable or unstable? |
|
Definition
| An established lysogen is typically stable. |
|
|
Term
| Give an example of a way that someone can induce an established lysogen to enter the lytic cycle. |
|
Definition
| UV light can induce expression of host genes. One of the host genes, the RecA, stimulates the cleavage of the lambda repressor, and thus, crippling lysogeny and promoting lytic growth, |
|
|
Term
| What does prophage induction involve? |
|
Definition
| the release of a repressor (such as the lambda repressor) from DNA |
|
|
