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Mol Dx Ch. 3 - Study Questions
Proteins
11
Biochemistry
Undergraduate 4
02/10/2014

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Term
Indicate whether the following peptides are hydrophilic or hydrophobic:
a. MLWILAA
b. VAIKVLIL
c. CSKEGCPN
d. SSIQKNET
Definition
a. hydrophobic
b. hydrophobic
c. hydrophilic
d. hydrophilic
Term
Indicate whether the following peptides are hydrophilic or hydrophobic:
e. YAQKFQGRT
f. AAPLIWWA
g. SLKSSTGCQ
Definition
e. hydrophilic
f. hydrophobic
g. hydrophobic
Term
Is the following peptide positively or negatively charged at neutral pH?

GWWMNKCHAGHLNGVYYQGGTY
Definition
Positively charged
Term
Consider an RNA template made from a 2:1 mixture of C:A. What would be the three amino acids most frequently incorporated into the protein?
Definition
proline, histidine, threonine
Term
What is the peptide sequence encoded in AUAUAUAUAUAUAUAUAUAUA...?
Definition
IYIYIYI...
Term
Write the anticodons 5' to 3' of the following amino acids: L, T, and M
Definition
leucine anticodons: UAA, CAA, AAG, GAG, UAG, CAG
threonine anticodons: AGU, GGU, UGU, CGU
methionine anticodons: CAU
Term
Write the anticodons 5' to 3' of the following amino acids: H, R, and I
Definition
histidine anticodons: AUG, GUG
arginine anticodons: ACG, GCG, UCG, CCG
isoleucine anticodons: AAU, GAU, UAU
Term
A histone-like protein contains the sequence PKKGSKKAVTKVQKKDGKKRKRSRK. What characteristic of this sequence makes it likely to associate with DNA?
Definition
It is positively charged, which makes it easier to associate with negatively charged DNA
Term
A procedure for digestion of DNA with a restriction enzyme includes a final incubation step of 5 minutes at 95 degrees C. What is the likely purpose of this final step?
Definition
The incubation will inactivate the enzyme.
Term
What is a ribozyme?
Definition
It is an RNA molecule that can metabolize other molecule as an enzyme does.
Term
Name the nonprotein prosthetic groups for the following conjugated proteins:
- glycoprotein
- lipoprotein
- metalloprotein
Definition
sugars, lipids, metal atoms
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