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Mol Dx Ch. 2 - Study Questions
Fundamentals of RNA
16
Biochemistry
Undergraduate 4
02/07/2014

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Term
Draw the secondary structure of the following RNA. The complementary sequences (inverted repeat) are underlined. 5' CAUGUUCAGCUCAUGUGAACGCU 3'
Definition
[image]
Term

Underline two inverted repeats in the following RNA:

5' CUGAACUUCAGUCAAGCAAAGAGUUUGCACUG 3'

Definition
5' CUGAACUUCAGUCAAGCAAAGAGUUUGCACUG 3'
Term
Name three processing steps undergone by messenger RNA.
Definition
Polyadenylation, capping and splicing
Term
What is the function of polyadenylate polymerase?
Definition
It digests RNA and adds the poly-A tail to the end of mRNA.
Term
What is unusual about the phosphodiester bond formed between mRNA and its 5' cap?
Definition
The 5' cap is attached by an unusual 5' to 5' pyrophosphate linkage.
Term
The parts of the hnRNA that are translated into protein (open reading frames) are the _______.
Definition
Exons
Term
The intervening sequences that interrupt protein coding sequences in hnRNA are _________.
Definition
Introns
Term
Proteins that bind to DNA to control gene expression are called _____ factors.
Definition
trans (transcription)
Term
The binding sites on DNA for proteins that control gene expression are _________ factors.
Definition
cis
Term
How might a single mRNA produce more than one protein product?
Definition
It may be alternatively spliced to produce different products.
Term
The type of transcription producing RNA that is continually required and relatively abundant in the cell is called _________ transcription.
Definition
constitutive
Term
A set of structural genes transcribed together on one polycistronic mRNA is called an __________.
Definition
operon
Term
Given the depiction of the lac operon in the text and in our powerpoints, indicate whether gene expression would be on or off in the following conditions (P=promoter,o=operator,R=repressor):
a. P+ O+ R+, no inducer
b. P+ O+ R+, inducer present
c. P- O+ R+, no inducer
d. P- O+ R+, inducer present
Definition
a. off
b. on
c. off
d. off
Term
Given the depiction of the lac operon in the text and in our powerpoints, indicate whether gene expression would be on or off in the following conditions (P=promoter,o=operator,R=repressor):
e. P+ O- R+, no inducer
f. P+ O- R+, inducer present
g. P+ O+ R-, no inducer
h. P+ O+ R-, inducer present
Definition
e. on
f. on
g. on
h. on
Term
Given the depiction of the lac operon in the text and in our powerpoints, indicate whether gene expression would be on or off in the following conditions (P=promoter,o=operator,R=repressor):
i. P- O- R+, no inducer
j. P- O- R+, inducer present
k. P- O+ R-, no inducer
l. P- O+ R-, inducer present
Definition
i. off
j. off
k. off
l. off
Term
Given the depiction of the lac operon in the text and in our powerpoints, indicate whether gene expression would be on or off in the following conditions (P=promoter,o=operator,R=repressor):
m. P+ O- R-, no inducer
n. P+ O- R-, inducer present
o. P- O- R-, no inducer
p. P- O- R-, inducer present
Definition
m. on
n. on
o. off
p. off
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