Term
| What color are Serratia marcescens colonies on TSA plates: |
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Definition
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Term
| True or False : A filamentous colony is perfectly circular and self - contained? |
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Definition
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Term
| When bacteria grows in broth and is equally spread out, it is called: |
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Definition
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Term
| You control the amount of light reaching your specimen on the stage by using the : |
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Definition
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Term
| True or False : Candida albicans is an Eukaryote |
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Definition
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Term
| True or False : Eukaryotes are generally larger organisms than Prokaryotes. |
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Definition
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Term
| Staphylococcus aureus cells are arranged in: |
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Definition
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Term
| Teh total magnification when using a 40x objective and 10x eyepiece on a microscopeis: |
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Definition
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Term
| Why do we bleach the bench tops before and after lab? |
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Definition
| So that we don't contaminate our samples with previous microbes on the table surface or leave any after lab |
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Term
| True or False : Bacillus subtilis is a Gram negative microbe |
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Definition
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Term
| The correct way to write the following species is(italics are analogous to underlining): |
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Definition
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Term
| When putting your microscope away, what is something you are NOT to do: |
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Definition
| Wipe oil off the oil objective lems with a Kimwipe |
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Term
| Which of the following is NOT a cluture mdedium we have used in this class: |
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Definition
| Clear Gelattin slant (CGS) |
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Term
| True of False : We transfer organisms using only sterile techniques. |
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Definition
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Term
| True or False : Pure cultures are only one species of microorganisms. |
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Definition
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Term
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Definition
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Term
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Definition
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Term
| The correct amount of time to wait to transfer bacteria after you have flame - sterilized your loop is: |
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Definition
| A few moments, and when the medium doesn't sizzle when you touch it |
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Term
| True or False : We evaporate the liquid on our loop before putting it in the falame to prevent aerosols. |
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Definition
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Term
| True or False : The correct way to open test tubes when you are inoculating them is to have your partner do it. |
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Definition
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Term
| The objective lens that uses immersin oil is: |
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Definition
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Term
| If your field of vision is too dim when looking in a microscope a way to correct this is to: |
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Definition
- Raise the condenser
- Open the iris diaphragm
- Be sure that the objective is in the place, over the stage
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Term
| True or False : You should move your inoculating loop in a zipzag pattern when inoculating a slant? |
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Definition
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Term
| True or False : Colony and cell morphology will allow you to determine species identification |
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Definition
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Term
| Plates need to be incubated upside down because: |
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Definition
| It prevents moisture from dripping on the cells and distributing them. |
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Term
| True or False : Individuals that are immunocompromised are less susceptible to the normal microbes around them. |
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Definition
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Term
| The first person to observe microorganisms was: |
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Definition
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Term
| The type of microscope we use in lab is: |
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Definition
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Term
| When using the microscope for measurement at 1000x, one division is approximately equal to: |
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Definition
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Term
| True or False: When beginning to look at a specimen in the microscope, you should start with the stage all the way down and move it up until the image comes into focus. |
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Definition
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Term
| True or False : Escherichia coli is a Gram negative coccus |
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Definition
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Term
| In the case of microbes, the term "ubiquitous" means that: |
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Definition
| Microbes are found on all types of surfaces and environments in this world |
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Term
| When Bacillus subtilis is grown on agar media, it: |
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Definition
| is cream-colored with a lighter center |
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Term
| This organism can cause the Toxic Shock Syndrome (TSS) in humans by releasing a dangerous exotoxin. We observed it under the microscope. This organism is: |
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Definition
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Term
| When looking at the arrangement of microbial cells, the term "strepto" means: |
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Definition
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Term
| Which is NOT a typical laboratory procedure? |
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Definition
Sterilize bench
Put unused media back onto shelves
Wash hands
Put all disposable/contaminated material in the biohazard bags after use |
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Term
| True or False : You should use the coarse objective adjustment knob after fouxing at 40x power. |
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Definition
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Term
| True or False : Growth patters in broth can indicate the oxygen requirements for organisms. |
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Definition
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Term
| True or False : Turbidity in a TSB broth tube indicates that it is sterile. |
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Definition
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Term
| True or False : A medical scientist who studies the disease and transmission of organisms is an epidemiologist. |
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Definition
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Term
| True or False : A colony arises from several different kinds of bacterial cells. |
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Definition
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Term
| When performing a cell culture transfer, it is important to: |
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Definition
- Flame the opening of the culture tube before and after opening it
- Flame the loop before the transfer
- Use aseptic technique
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Term
| When agar media is used in test tubes, it is referred to as: |
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Definition
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Term
| True or False: Solid Media are essential for the separation of bacteria from specimens containing a mixture of organisms |
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Definition
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Term
| To prevent aerosols from forming: |
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Definition
| Hold the loop just outside the flame until all liquid evaporates and then place the loop in the flame. |
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Term
| What would be a possible correction if the microscope field is too dim? |
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Definition
- Raise the condenser
- Open the iris diaphragm
- Be sure the objective is in the place, directly over the stage
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Term
| What possible correction would you do if the microscope has particles of dust or lint blocking your view? |
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Definition
| Wipe ocular and objective with lens paper |
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Term
| What possible correction would you do if the microscope has floating particles in a hazy field? |
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Definition
- To little oil, so the objective is not completely submerged, add oil
- Bubbles in the oil, add more oil (be sure the high dry lens in not in oil
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Term
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Definition
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Term
| What shape are B. Bacilli? |
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Definition
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Term
| What shape are C. Spirilla? |
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Definition
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Term
| What is the purpose of washing the laboratory bench top with disinfectannt before transferring cultures? |
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Definition
| Microorganisms are ubiquitous. We must learn to handle them asecptically, techniques that prevent contamination. Cultures must not be contaminated with organisms from the lab environment, if they are, your experimental results will be erroneous. |
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Term
| How could you determine if the culture media given to you was sterile? |
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Definition
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Term
| What are the signs of bacterial growth in broth media? |
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Definition
- Uniform clouding of the broth
- small puffballs floating in the broth
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Term
| What are the components of Tryptic Soy Agar? |
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Definition
- casein
- soybean meal
- glucose
- sodium chloride
- Dipotassium phosphate
- Agar
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Term
| What are the components of Blood Agar? |
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Definition
- mammalian blood (usually sheep or horse), typically at a concentration of 5–10%
- tryptone
- agar
- sodium chloride
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