Term
| Use enterotubes for which bacteria? |
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Definition
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Term
| 2 properties of enteric bacteria? |
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Definition
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Term
| # of media and biochemical tests in enterotube? |
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Definition
| 12 media, 15 biochemical tests |
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Term
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Definition
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Term
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Definition
| Ability of bacteria to take naked DNA from environment |
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Term
| What's TENS solution for? |
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Definition
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Term
| Horizontal gene transfer? |
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Definition
| Bacterium incorporates DNA from Unrelated (can be same species, but not a direct relative or ancestor) bacterium |
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Term
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Definition
| Bacterium receives genetic material from Relative. |
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Term
| Gene transfer for Transformation? |
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Definition
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Term
| 2 naturally competent bacteria? |
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Definition
| Streptococcus and Acietobacter |
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Term
| Steps of transformation experiment? |
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Definition
| Make POB and Glucose control plates and put ADP 6 and 1 into both. Put 1.5 mL ADP-1 in centrifuge for 2 min at 10k RPM. Discard supernatant, keep pellet. Put 300 microL TENS to pellet to lyse cells. Make serial dilutions. Prepare a POB plate with ADP-6 with spread plate technique. Divide to 4 quadrants. In quadrant 1, put 1 microL ADP-1 serially diluted by 10 --> quadrant 4, serially diluted by 10^(-3). Grow for 24 to 48 hrs. |
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Term
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Definition
| transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells |
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Term
| Difference between Transformation and Conjugation? |
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Definition
| Transformation, bacterium gets DNA from environment. Conjugation, bacterium gets DNA from another bacterium. |
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Term
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Definition
| Extrachromosomal genetic element that occurs in many bacterial strains. Circular DNA, replicate independently from bacterial chromosome. Not essential for bacterium but may be an advantage. |
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Term
| Why no growth in E. coli streak on test plate? |
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Definition
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Term
| Difference between Auxotroph and Prototroph? |
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Definition
| Auxotroph, can't make a particular organic compound needed for its growth. Prototroph, can make all compounds needed for growth. |
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Term
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Definition
Tris buffer,
Ethylenediaminetetraacetic acid,
NaOH,
Sodium dodecyl sulfate |
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Term
| Gene transfer for Conjugation? |
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Definition
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Term
| Why more growth at 1/10 dilution than full strength? |
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Definition
| There was still leftover TENS solution in full strength, so it killed some ADP-6 bacteria. Less TENS solution in 1/10 dilution, so didn't kill bacteria. |
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Term
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Definition
| Gene in cell's plasmid that can make pilus and thus perform conjugation |
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Term
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Definition
| Gene to form pilus is missing in bacteria's plasmid, thus cannot perform conjugation. |
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Term
| Leu-, Tol+ bacteria grow in Tol media w/out Leu? |
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Definition
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Term
| Leu-, Tol+ bacteria grow in glucose minimal media? |
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Definition
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Term
| Leu-, Tol+ bacteria grow in Leu media w/out Tol? |
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Definition
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Term
| Leu-, Tol+ bacteria grow in nutrient broth? |
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Definition
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