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| A sexual process of reproduction in which one cell divides into daughter cells; aceptic production. |
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| A population of organisms descended from a single cell |
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| M/O that grows best in the cold |
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| M/O that has an optimum temp between 20-30 degrees celcius. (warm) |
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| Bacteria that grows most rapidly at temps between 20-40 degrees celcius (warm-hot). |
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| Oraganism with an optimum growth temp between 45-70 degrees celcius (hot hot) |
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| Organisms that require oxygen |
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| Organisms that can't multiply if oxygen is present |
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| Organism that grow best with in the presence of oxygen but can learn to grow in its absence. |
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| Organism that requires a small amount of oxygen (2%-10%) but can't grow in higher concentrations of oxygen |
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| Organisms that can grow in the presence of oxygen but never use it as a terminal electron acceptor. |
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| Organisms that prefers high salt medium to grow in. |
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| organisms that require increased concentrations of carbon dioxide (5%-10%) and approx. 15% oxygen. |
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Bacteriological medium that contains protein digests, extracts, or other ingredients that vary in chemical
composition
"a tasty soup for microbes"
One common ingredient is peptone and extracts |
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| a mix of amino acids and short peptides |
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| Water soluble compostion of a substance. |
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Bacteriological medium to which addition ingredients have been added that inhibit growth of many organisms other than the one being sought.
Example: MAC
grows gram neg/inhibits gram pos
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Bacteriological medium that contains ingredients that can be changed by certain bacteria in a recognizable way
pH indicator
Example: Blood agar testing alpha beta or gamma |
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Another method to cultivate anaerobes incorporates this into the culture medium.
These react with oxygen and thus eliminates the oxygen
Contains an oxygen indicating dye that turns blue in the presence of oxygen |
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CHOMPS:
Carbon, Hydrogen, Oxygen, Magnesium, Phosphorus, Sulfur |
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Required in minute amounts in cells
Cobalt, Zinc, Copper, Molybdenum, and Maganese.
Are required for enzyme function |
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| Growth Factors for microbial growth |
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| Amino Acids, vitamins, purines, and pyrimidines |
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Synthesis of macromolecules required for multipication and they can generate energy in the form of ATP.
Time for this phase varies |
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Cells divide at a constant rate
Most susceptable to antibiotics at this phase |
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Cells no longer have stores of energy and enough nutrients for growth
at this phase some cells are multiplying while some are dying |
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| Cells die of at a constant rate. |
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Direct count
Count Total number of cells: Dead and alive
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| counting only living cells |
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| concentrates cells that were in a diluted environment and counts the living cells |
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broth in test tube is cloudy
use the spectrophotometer to measure
Counts living cells |
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| the cell has a higher concentration on the outside and shrivels up |
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More concentrated on the inside of the cell
the cell looks like its going to explode |
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| Occurs in a hypertonic solution |
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