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Microbiology
Bacteria
86
Biology
Undergraduate 3
11/01/2006

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Term
Carl Woese
Definition
-derived systematic nomenclature and built taxonomy
-based on 16S of 30S
Term
Three life domains
Definition
-bacteria
-archaea
-eukarya
-drives microbiology organization
Term
Bible of Bacteriology
Definition
Bergey's Manual
Term
Ectothiorhodospora
Definition
-Purple Sulfur
-photoautotroph
-oxidizes sulfur and deposits it on the cell
-H2S-->S(0)-->So4(-2)
-found in saline lakes, soda lakes, and salterns
Term
Rhodo Brothers
Definition
-Purple Non-Sulfur Bacteria
-photoheterotroph
-don't fix CO2
-ferment in dark
Term
Acetobacter
Definition
-CH3 CH2OH//O2-->CH3COOH
-respires alcohol to acetic acid
-fruit spoiling aerobically produces vinegar
Term
Ecology and Industrial Issues
Definition
-found in alcoholic jouces
-CaCO3 plates
-incomplete oxidation of organic compounds
-synthesize cellulose
Term
Neisseria
Definition
-Gm- coccus (unusual)
-Gonorrhea and Meningities
Term
Chromobacterium
Definition
-rod-shaped
-Violaceum--purple pigment
-very resistant to antibiotics
-bone/sinus infections with purple ooze
-malignant
-very clean H2O
Term
Vibrio
Definition
-cholera
-deep sea fish
-bent rods
-OXIDASE POSITIVE
Term
Photobacterium and Bioluminescence
Definition
-marine, associated with fish
-grow in special organ or in dead fish
-facultative aerobes
-luciferase, reduced flavin mononucleotide, and O2 required
Term
Autoinduction
Definition
-luciferase regulatory synthesis
-produce autoinducer
-quorum sensing
Term
Regulation of Bioluminescence
Definition
-only when conditions are favorable for the development of high population densities
Term
Rickettsias
Metabolism and Pathogenesis
Definition
-intracellular
-Rocky Mountain Spotted Fever
-Epidemic typhus
-oxidize glutamate or glutamine
-respiratory chain with cytochromes
-electron transport phosphorylation
-rest of nutrients from cell
-transmitted by arthropod vectors
-penetrate epithelial cells of GTI, multiply, appear in feces
Term
Wolbachia
Definition
-rod-shaped, intracellular in arthropods
-parthenogenesis, killing of males, and feminization (males develop into females)
-colonize in insect egg
Term
Nitroso
Definition
-nitrosifying
-chemolithotrophs
-utilize inorganic electron donors
-ammonia oxidizing
-ammonia monooxygenase
Term
Nitro
Definition
-chemolithotrops
-nitrite-oxidizing bacteria
-nitrite oxidase
Term
Ecology Isolation and Culture of Nitrifying Bacteria
Definition
-soil and water
-high ammonia content
-mineral salts media
Term
Thiobacillus
Definition
-rod-shaped
-acidophilic
-Fe+2//O2-->Fe+3
-H2S + 2O2-->SO42- + 2H+
-S(0) + H2O + 1 1/2 O2 --> SO42- + 2H+
S2O3 2- + H2O + 2O2 --> 2SO4 2- + 2H+
Term
Acidothiobacillus ferroxidans
Definition
live by oxidizing iron
Term
Beggiotoa
Definition
-Fe+2//O2-->Fe+3
-filamentous, gliding, sulfur-oxidizing
-many cells from end to end
-habitats rich in H2S
-Winogradsky's 1st
-chemolithotropic autotrophs
-rhizosphere of plants
-settling problems in sewage
Term
Thioploca
Definition
-sulfur-oxidizing
-chemolithotrophic
-all bundles surrounded by sheath
-anoxic oxidation of H2S
-accumulate huge amounts of nitrate and support extended periods of anaerobix resp w/ H2S as e- donor
Term
Ralstonia
Definition
-H2//O2-->H2O
-H2S//O2-->SO4-2
-H2 sole e- donor
-O2 sole e- acceptor
-Knallgas reaction
-more than 1 hydrogenase
-facultative
-hydrogen oxidizing
Term
Physiology and Ecology of "H" bacteria
Definition
-grow best under microoxic conditions
-5-10% of O2 levels
-nickel in media
Term
CO Oxidation (Pseudomonas and carboxydovorans)
Definition
-grow on CO
-carboxydotrophic
-Calvin cycle Rxns
-Co -> CO2 by codehydrogenase
-pterin bound to ^
Term
Pseudomonas carboxydovorans
Definition
-can grow anaerobically with natrate as e- acceptor
Term
C1 Metabolism in Methanotrophys and Methylotrophs
Definition
-no C-C bonds
-require O2 as reactant in oxygenation
-obligate Carbon
-methanotrophs have sterols = essential part of internal membrane system in Ch4 oxidation
-methanotrophs have methane monooxygenase
Term
TYPE 1 Methylotrophs
Definition
-assimile on C via ribulose monophosphate cycle
-disc shaped vesivales
- lack of complete CAC
- obligate methylotrophs
Term
TYPE 2 Methylotrophs
Definition
-assimilate c1 intermediate via serine pathway
-paired membranes
-hold metahne monooxygenase
Term
Methanotrophs
Definition
-oxidize methane
-Ch4 = e- donor
-aerobes
-soil and water
-grow with one-c organic compound and methane
Term
Methylotrophs
Definition
oxidize methane
grow with one carbon organic compound
Term
Ecology and Isolation of Methano and Methylo trophs
Definition
aquatic and terrestrial environments
METHANE!!
narrow badn at oxycline
mineral salt medium 80% methane 20% air
Term
Methanotrophic Symbionts of Animals
Definition
-relationships with mussels and sponges
-hydrocarbon seeps on teh seafloor, places where methane is released
-mussel gills consume methane where TYPE 1 Methanotrophs are located
Term
Pseudomonads
Definition
-straight or slightly curved cemoorganotrophic aerobic rods with polar flagella
-absence of gas formation from glucose
-catalase positive
-OXIDASE POSITIVE
-0.5-1.0 micrometer
-no spores
-no sheaths
-never fermentative
-H2 or CO sole e- donor
-nitrate e- acceptor anaerobically
-responsible for breakdown in oxic habitats
-
Term
Entner=Doudoroff Pathway
Definition
-6-phosphateglyconate dehydrase
-2-keto-3-deoxyglucosephosphate aldolase
-not in Gm+
Term
Zymomonas
Definition
-Fermentation of sugars to ethanol
-Spittle Beer
-Entner-Doudoroff pathway
-fermentation of agave
-yogurts, cheese
-homofermentive
Term
Azotobacter
Definition
-fastest rate of respiration
-N2 requires two reducing equivalents (ATP, Fd) because no living plant/animal can fix pure N
-Slime layers impede 02
-can decouple ETS to consume tremendous amount of oxygen
Term
Spirilla
Definition
-not pathogenic except Bdellovibrio
Term
Bdellovibrio
Definition
-prey on other Gm- bacteria
-develops intraperiplsmically
-froms a spehrical structure called a bdelloplast
-oxidation of amino acids and acetate
Term
Magneto Spirilla
Definition
-magnetotaxis
-orient long axis with northsouth magnetic field
-magnetosomes
Term
Campylobacter
Definition
-microaerophile
-bacterial diarrhea
-contaminates alomst 100% of poultry carcass
-most common cause of undiagnosed bacterial diarrhea
Term
Helicobacter
Definition
-acute and chronic gastritis
-fromation fo peptic ulcers
-related to campylobacter
Term
Sphaerotilus
Definition
-oxidize Fe+3
-clogs pipes because filaments tangle
-sheath
-filament is a chain of rod-shaped cells
-obligate aerobe
Term
Leptothrix
Definition
-SEWAGE FUNGUS
-precipitates Manganese
-sheath
Term
Caulobacter
Definition
-inside H2O treatment cartidges'
-like the Carbon
-cytoplasm filled stalk
-chemoorganotroph
-holdfast structure attaches to surface
Term
Gallionella
Definition
-twisted stalklike structure of iron
-bean shaped cells
-cannot be grown successfully
-might represent lithotrophy
-in neutral pH iron oxidation produces even smaller exergonic reactions
Term
Stigmatella
Definition
-largest genomes of any prokaryote
-fruiting body formation
-can form multicellular structures
-have reproduction
Term
Staphylococcus
Definition
-Oxidase "-"
-Catalase "+"
-facultative aerobe
-forms cell clusters
-commensals and parasites of humans and animals
Term
Micrococcus
Definition
-devil bugs that fall on plate
-found in air
-citochrome C oxidase in ETS
-Oxidase "-" Catalase "_"
-shows up as pure white or pink
-w/stand drying well
-obligate aerobe
-acid from glucose aerobically
-no cell clusters
Term
Streptococcus
Definition
-no respiratory activity
-heterofermental
-EM Pathway
-buttermilk, cheese, medical, etc.
-produce lactic acid
Term
Lactobacillus
Definition
-homofermenter
-pyruvate --> lactic acid
-antimicrobial properties
-Bulgarian buttermilk
-rod-shape
Term
Listeria
Definition
-make lactic acid
-plagues food industry (listeriosis)
-largest meat recall in '93
-likes fridge
-80-85% spontaneous abortion--crosses placental barrier
Term
Bacillus
Definition
-few are bad one causes eye infections
-form endospores
-harmless unless anthracis
-thurigienesis - bad for butterflies
-protoxin converted to toxin by prteolyic cleavage in the larval guy
-binds to intstinal epithelial cells and induces pore formation
-can clone into crops
Term
Clostridium
Definition
-found in soils
-makes endospores
-food poisoning--BOTULISM
--most potent biological poison
-1mg = >1 million rats dead
-tetanus
-pyruvate-->solvents
-ATP throuh substrate level phosphoryl.
-sugars--butyric acid
Term
Mycoplasma
Definition
-no cell wall
-wormy
-subject to osmotic shock
-obligate
-small genome - 500-1100 kbp
-steals steroids for their membranes
-M. genitalium
Term
Spiroplasmas
Definition
-parasitic to sucking insectes
-contain genes that appear to come from plants/insects
-helical or spiral shaped
-rotary motion makes them motile
Term
Corynebacterium
Definition
-arthrobacter
-weird shapes V-shaped
-diptheriae
Term
Propionibacterium
Definition
-Swiss Cheese!
-propionic acid
-thought fermenter
-membrane has a sodium translocating ATPase to pump sodium out
Term
Mycobacterium
Ziehl-Neelsen Stain
Definition
-fuchsin and phenol
-steam for 2-3 min to force dye into cell
-acid-fast = red
-non = blue
-mycolic acid necessary for acid fastness
Term
Mycobacteria
Definition
-pleomorphic
-slow growers and fast growers
-TB = slow
-tight, compact, wrinkled colonies
-simple mineral salt media
-form yellow carotenoid pigments
-classifications: nonpigmented, forming pigment in light, pigment in dark
Term
Streptomyces
Definition
-+500 species
-most clincal antibiotics
-found in soil
-excrete substances--poisons
-filaments are .5-1 micromete diamter, infinite length
-growth occurs at tips of filaments
-spores are called conidia
-conidia produced through formation of cross-walls
Term
Ecology and Isolation of Streptomyces
Definition
-primarily soil
-odor of soil from production of geosmins
-alkaline and neutral soil favorable
-well-drained soils
Term
Antibiotics of Streptomyces
Definition
-50% proved to be antibiotic producers
-500 antibiotic substances produced
-streptomycin, spectionmycin, neomycin
Term
Cyanobacteria
Definition
-old name blue-green algae
-oxygenic photosyntheseis
-Z scheme
-chlorophyll A
-phycobilins
-heterocyst (round, seemingly empty cells, along a filament or an end of one)
Term
Anaboena
Definition
-refractile like a diamond
-heterocyst fixes ntirogen (azoto)
-can blow up cell w/ a lot of O2
-no photosystem 2
-block O2 diffusion bcuz refractile
Term
Oscillatoria
Definition
-can fix nitrogen in dark
-danger of O22 eliminated
-nitrogenase closes in light
-changes quarternary structure to close in light
Term
Prochloron
Definition
-part of endosymbiotic theory
-first procholorophyte discovered
-marine invertebrates
-muramic acid --> peptidoglycan present
-carotenoids similar to cyanobacteria
Term
Life Cycle of Chlamydia
Definition
-Champion STD
-elementary body attacks host cell
-phagocytosis of elementary body
-conversion to reticulate body
-multiplication of reticulate bodies
-conversion to elementary boides
-release of elementary bodies
Term
Planctomyces
Definition
-lack peptidoglycan
***cells walls are S-layer***
-large amounts of cystine and proline
-resitant to penicillin nad cephalosporin
-
Term
Gemmata
Definition
-nuceloid surrounded by nculear envelope
-remains in a covalently closed, circular, and supercoiled form
-remains partitioned from remaingin cytoplasm by true unit membrane
Term
Verrucomicrobia
Definition
-oligotrophic--very little organic C and other energy sources
-form cytoplasmic appendages called prostheca
-two to several prostheca
-divide symmetrically
-peptidoglycan
Term
Prosthecobacter
Definition
-form prostheca
-two genes that show homology to tubulins of euk.
-similar to FtsZ
Term
Bacteroides
Definition
-anaerobes
-prevalent in human guy
-10^10/gm in human feces
-proteolitic
-produce abdominal infections
-synthesize shingolipids
Term
Flavobacterium
Definition
-aerobic
-pigmented rods
-F. meningosepticum = infant meningitis
-cultured on agar with cellulose fibers
-obligate aerobe
-fish pathogens
Term
Cytophaga
Definition
-gliding motility, but no flagella
-look like they crawl
-can eat cellulose, peptidoglycan
-
Term
Chlorobium
Definition
-Green Sulfur Bacteria
-anoxygenic phototroph
-S compounds e- donor
-chlorophyll acde
-chlorosomes (segregated structures on reaction center)
-sulfur resides outside cell
-consortium - associaton w/ chemoorganotrophic bacteria
Term
Spirochetes
Definition
-Gm-, motile, tightly coiled
-protoplasmic cylinder
-endoflagella (inside membrane)
-Skinny
-not spirella
Term
Spirochete Motility
Definition
-endoflagella
-both rotate is same direction
-torsion on cell
Term
Treponema
Definition
-Spirochete
-Syphilis
-never grown in vitro
-oral cavities
-hindgut of termites
Term
Borrelia
Definition
-Spirochete
-Lyme disease
-tick-borne
-some protozoa use spiros as flagella
-animal or human pathogens
-relapsing fever
-trasmitted through insect vector
-death in 40% infected
Term
Deionococcus
Definition
-15,000 Grays of radiation
-red or pink due to carotenoids-
-resistant to mutagenic effects due to DNA arrangement
-toroidal ring
-only effected by nirtosuguanidine
-efficeint in DNA repair
Term
Chloroflexus
Definition
-green nonsulfur
-hybrid phototroph
-thermophilic
-lack peptidoglycan
-earliest known phototrophic bacterium
Term
Physiology of Chloroflexus
Definition
-resemble purple nonsulfur in photoautotrophy
-grows well in dark by aerobic respiration as chemootrangotroph
-hydroxyproponate pathway
Term
Differibacter
Definition
-composed of species that specialize in anaerobic energy metabolism
-extremely versatile at anaerobic respiration using a variety of electron acceptors, including the metals Fe3+ and Mn4+
-barrophiles and thermophiles
-associated with mineral deposits
Term
Thermomicrobium
Definition
-chemotrophic member
-nothing to do with glycerol
-form bilayers
-hyperthermophile
-aerobic
-membrane lipids contain 1,2 dialcohols
-neither ester nort ether linkages
-lack peptidoglycan
Term
Thermodesulfobacterium
Definition
-thermophilic, sulfate reducing bacterium
-growth temperature optimum is only 70 degrees Celsius
-ether-linked lipids
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