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Microbiology
Test 2 - Study guide 2 questions 1-27
39
Microbiology
Undergraduate 4
11/06/2011

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Term
What are the six most common chemical elements found in living organisms?
Definition
Carbon, Hydrogen, Oxygen, Nitrogen, Phosphorus, Sulfur
Term
Metabolism
Definition
the ability of an organism to get energy from the environment and use that energy to grow.
Term
Catabolism
Definition
the process of breaking large compounds (that contain energy) to release smaller compounds (releasing energy)

- Energy gets released AS HEAT and STORED AS ATP
Term
Anabolism
Definition
the process of taking smaller molecules (amino acids) and make large compounds (proteins).

*This requires the energy that was released from the catabolic reactions.
Term
Redox Reactions
Definition
moving of an e- from an electron donor to an electron acceptor, as electrons move from donor to the acceptor, the acceptor becomes more energy rich
Term
Oxidation reaction
Definition
the electron DONATING half of a redox reaction
Term
Reduction reaction
Definition
the electron ACCEPTING half of a redox reaction
Term
Catalyst
Definition
substances that increase the rate of a chemical reaction without being permanently altered itself.
- lowers the activation energy needed for a chemical reaction to occur.
Term
Activation energy
Definition
energy that is required to bring the reacting molecules together in the correct way to reach the transition state
Term
catalysts
Definition
Enzymes are the ------- of biological reactions.
Term
Enzymes
Definition
What reduces the activation energy required to start a particular reaction?
Term
Substrate concentration, temperature, pH.
Definition
Three factors that can effect enzyme activity.
Term
They break the HYDROGEN BONDING (denature the enzyme) that holds the 3-D shape together.
Definition
Why do temperature and pH affect enzyme activity
Term
co-factor
Definition
some enzymes require metals to be close to the activation site
Term
Mg, Fe, Cu, Zn
Definition
examples of co-factors (metals close to the activation site of an enzyme)
Term
co-enzyme
Definition
Some enzymes require complex organic molecules to be near the activation site.
Term
NAD+, NADP+ (both e- carriers)
Definition
examples of a co-enzyme (complex organic molecules near an enzyme activation site)
Term
inhibitors
Definition
Enzyme activity can be regulated by -------.
Term
Competitive inhibitors
Definition
these LOOK LIKE the substrate so they bind to active site of enzymes therefore preventing the substrate from binding...
Term
Non-competitive inhibitors
Definition
binds to the ALLOSTERIC site on the enzyme which causes the enzyme to change shape and will no longer function.
Term
Oxidation reaction with co-enzyme NAD
Definition
What type of reaction is a dehydrogenase involved with?
Term
Catalyzes the phosphorylation of a substrate by ATP
Definition
What type of reaction is a kinase involved with?
Term
Removal of CO2
Definition
What type of reaction is a decarboxylase involved with?
Term
Heterotroph
Definition
oxidizes organic compounds (lipids, carbs, proteins, sugars. These organic compounds serve as a Carbon source and energy source.
Term
Photoautotroph
Definition
uses light as energy source via photosynthesis. Because light has no carbon source, CO2 is converted into glucose which is used for metabolism.
Term
Chemoautotroph
Definition
Oxidizes reduced forms of inorganic compounds. Reduced forms of inorganic compounds serve as energy source (H2/H2S/Fe2+) The organism gets its Carbon source from CO2 in the env't (ex. Lithotrophs "rock eating") ::BACTERIA ONLY::
Term
Heterotrophs
Definition
Humans and human pathogens would both be....
Term
Fermentation
Definition
partial oxidation of organic compounds
Term
1) Glycolysis. 2) 2 ATP, 2 NADH.
Definition
1. What biological pathway is used during Fermentation? 2. How many ATP and NADH are produced per glucose?
Term
Glycolysis
Definition
a catabolic process in which glucose (6 carbons) breaks down into 2 pyruvate (3 carbons)
Term
ethanol and lactic acid
Definition
two examples of Fermentation end products
Term
aerobic respiration
Definition
complete oxidation of glucose to CO2 and H2O
Term
38 ATP/glucose. Oxidative phophorylation, glycolysis, Kreb's cycle, ETS
Definition
How many ATP are produced during Aerobic Respiration? What are the biochemical pathways used?
Term
2 ATP, 6 NADH, 2 FADH2, 4 CO2
Definition
What is all produced during the krebs cycle?
Term
Chemiosmosis
Definition
The movement of H+ across the membrane through ATPase. Protons alone can NOT diffuse across the membrane so ATPase acts as a gate to allow this to occur.
Term
Cell membrane
Definition
where is the ETC found in Bacteria?
Term
Mitochondria
Definition
Where is the ETC found in eukaryotes?
Term
ATPase diffuses H+ across the membrane (chemiosmosis), ATPase uses the H+ to fuel the change from ADP to ATP
Definition
What does the enzyme ATPase do?
Term
Oxygen is the final electron acceptor
Definition
What is the role of oxygen in the ETC
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