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| two or more stains used in a procedure, allows detection of differences between several different organisms or differences between parts of the same organism. |
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| most frequently used differential stain in microbiology |
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| Steps in Gram Stain Procedure |
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Definition
| primary stain, mordant, decolorizer, counterstain |
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| 75% ethyl alcohol/25% acetone |
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| Thickness of the peptidoglycan layer allows ... |
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Definition
| bacteria to be categorized as Gram positive or Gram negative |
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| Using crystal violet, Gram positive should be ... and Gram negative should be... |
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Definition
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| Using Gram's Iodine, Gram positive should be... and Gram negative should be... |
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| Using the Decolorizer, Gram positive should be... and Gram negative should be... |
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| Using Safranin, Gram positive should be... and Gram negative should be... |
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| After staining, Staphylococcus aureus is a Gram... organism and appeared... |
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Definition
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| After staining Pseudomonas aeruginosa is a Gram... and appeared... |
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| After staining, Bacillus megaterium is a Gram... and appeared... |
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| State the reasoning behind the "24 hr culture"? |
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Definition
| after 24 hours of growth, Gram results is unreliable because the cell wall breaks down |
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| What part of the bacterial cell (cell wall or cell membrane) appears to play the most important role in determining the Gram reaction? |
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| What color would you expect Staphylococcus to be if the Gram stain procedure was stopped after the Gram's Iodine step? |
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| What color would you expect Pseudomonas to be if the Gram stain procedure was stopped after the Gram's Iodine step? |
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Definition
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| What color would you expect Pseudomonas to be if the Gram stain procedure was stopped after the Decolorizer step? |
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Definition
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| Why would stain like methyline blue not work just as well as Safranin for counterstaining in the Gram stain procedure? |
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Definition
| the counterstain has to be a contrasting color |
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| Which step in the Gram stain procedure needs to be most carefully watched? How do you know when it is done? |
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Definition
| the Decolorizer, til the decolorizer runs clear |
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| What cellular structure allows the bacterial cell to exhibit motility |
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Definition
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| Can you see the flagellum? Why or why not? |
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Definition
| not really, it tends to be very small |
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| What is the difference between true motility and Brownian movements (which are water molecules colliding with the bacteria? |
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Definition
| true motility seems to be more purposeful |
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| Did you notice any bacteria swimming in "runs and tumbles"? |
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| Did you notice any bacteria swimming more or less in a straight line? |
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