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Microbiology
Pages 21 - 26
26
Microbiology
Undergraduate 1
02/07/2019

Additional Microbiology Flashcards

 


 

Cards

Term
Cycle of Sporulation and Germination
Definition
a vegetative cell's environment is unfavorable
sporulation
spore
germination
vegetative cell functions normally
Term
Dipicolinic acid
Definition
a compound found in spores that is the liely culprit for their resistant living structures
Term
To destroy an endospore ...
Definition
autoclave
discontinuous sterilization
Term
A spore is largely unaffected by ...
Definition
disinfectant/antiseptics
heat
radiation
drying
time
Term
Endospore formation takes place after a cell has grown for some period as a .... cell and the cell has begun to run out of nutrient and build up wastes
Definition
vegetative
Term
Autoclave...
Definition
250 degree heat and 15 psi pressure
Term
Discontinuous Sterilization
Definition
boil... cool... boil... cool... boil... cool
Term
Endospores are produced by two medically significant Genera...
Definition
Bacillus and Clostridium
Term
The endospore allows the bacterial cell to survive - but remain metabolically ... until environmental conditions improve to allow the organism to grow again
Definition
dormant
Term
Sporulation
Definition
endospore formation (occurs within 6-8 hours)
Term
Spore
Definition
until favorable environmental conditions return, may stay in "spore" state for extended periods of time
Term
Germination
Definition
vegetative cell emerges from spore when conditions permit (2-3 hours)
Term
Anthrax - organism, damage by, and introduced by
Definition
Bacillus, toxins, skin and lungs
Term
Botulism - organism, damage by, introduced by
Definition
Clostridium, toxins, ingestion
Term
Tetanus - organism, damage by, introduced by
Definition
Clostridium, toxins, wound
Term
Gas Gangrene - organism, damage by, introduced by
Definition
Clostridium, toxins, necrotic
Term
Method of bacterial reproduction is called...
Definition
binary fisson
Term
Steps in binary fisson
Definition
DNA replicates
bacterial cell elongates
"cross wall" forms
cytoplasm splits equally
each "new" cell has identical DNA
cells grow and divide again
Term
Geration time
Definition
(also called doubling time) time it takes until new cells will divide again
Term
What limits binary fisson?
Definition
lack of nutrients
lack of space
buildup of wastes
Term
Bacterial Growth Curve phases
Definition
lag. log, stationary,  death
Term
Lag Phase of the Bacterial Growth Curve
Definition
occurs during innoculation
cells become acclimated
very little cell division
Term
Log Phase of the Bacterial Growth Curve
Definition
rapid cell division
cell numbers increase exponentially
symptoms of disease appear
ATBs are most effective
antiseptics/disinfectants most effective
Term
Stationary Phase of the Bacterial Growth Curve
Definition
also called the equilibrium phase
balance between growth of new cells and death of old cells
nutrients begin to be depleted
waste builds up
spores may begin to form
Term
Decline Phase of Bacterial Growth Curve
Definition
also called the death phase
death rate is much faster than fission rate
colony population begins to die out
disease begin to resolve themselves
some cells live longer is they form spores or possess flagella
Term
Equation for the Bacterial Growth Curve
Definition
Bf = Bi x 2n
Bf = final number of bacteria
Bi = initial population of bacteria
n = number of generations
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