Term
| what does starch hydrolysis test for? what end products? what stain used? |
|
Definition
1.amylase enzyme
2.glucose, dextrins, maltose
3.Iodine |
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Term
| what color is mannitol salt agar? what does yellow color mean? |
|
Definition
1.red agar
2. able to ferment mannitol AND tolerate 7.5% salt |
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Term
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Definition
| test tube with smaller, inverted test tube inside to collect gas |
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Term
| what color is EMB agar? what does it stand for? what does it test for? what do pos. and neg. results look like? |
|
Definition
1.purplish
2.Eosin methylene blue
3.Lactose fermentation
4.(+)=colored colonies, (-)=clear colonies |
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Term
| which test uses Kovacs reagent? |
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Definition
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Term
| what is indole? where found? |
|
Definition
| nitrogen containing organic compound found in amino acid tryptophan? |
|
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Term
| what does indole test test for? what is positive result? how many drops of reagent? |
|
Definition
1.ability to ferment amino acid tryptophan
2.(+)=red layer on top
3.3 drops of Kovacs reagent |
|
|
Term
| what are the end products of tryptophan? |
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Definition
| indole, pyruvate, ammonia |
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Term
| what type of pyruvate endproducts detected by methyl red test? examples? what is pos. and neg. result? what reagent added? |
|
Definition
1.acidic
2.acetic acid, lactic acid, succinic acid
3.(+)=red, (-)=yellow4.Methyl Red Indicator |
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Term
| what does the Voges Proskauer test detect? what is the pH? what type of fermentation? |
|
Definition
acetoin (acetylmethylcarbinol)
neutral
Butanediol |
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Term
| what test uses alpha-naphthol and potassium hydroxide? how many drops of each? how long do you wait for results? what is pos/neg result? |
|
Definition
Voges Proskauer
8 drops each
5-30 minutes
pink/red=(+), no color change=(-) |
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Term
| what does urea test test for? what end products if present? what color is pos.? |
|
Definition
urease enzyme
ammonia, water, CO2
dark red=(+) |
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|
Term
| what test uses sulfanilic acid? |
|
Definition
|
|
Term
| what test uses potassium hydroxide? |
|
Definition
|
|
Term
| what test uses Dimethyl-alpha-naphthylamine? |
|
Definition
|
|
Term
| what test uses Nessler's reagent? |
|
Definition
|
|
Term
| what test uses apha-naphthol? |
|
Definition
|
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Term
| How many days needed for nitrate test? what products tested for? which reagents for which products? what are positive colors? how many drops for 1mL spot plate sample? |
|
Definition
4-6 days
nitrite and ammonia
sulfanilic acid + dimethyl-alpha-naphthylamine for nitrite--Nessler's for ammonia
pink/red=nitrate(+), yellow/brown=(+)ammonia
3 drops acid, 2 drops d.a.n, several Nessler's |
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|
Term
| what does Triple Sugar Iron agar test for? |
|
Definition
| glucose fermenation, sucrose and/or lactose fermentation, H2S production, aerobic capacity |
|
|
Term
| what does a yellow TSI test mean? |
|
Definition
| acidic products from lactose and/or sucrose fermentation |
|
|
Term
| what causes alkaline reversion in TSI test? |
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Definition
| only the small amount of glucose is able to be metabolized, so organism begins breakdown of acidic end products, resulting in alkaline products (yellow acid result begins to turn pink) |
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|
Term
| what is the sign of H2S production in TSI test? |
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Definition
|
|
Term
| what does Lysine agar test for? |
|
Definition
| H2S production, glucose fermentation, ability to decarboxylylate of deaminiate lysine |
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Term
|
Definition
|
|
Term
| what color LIA test means the deamination has taken place? |
|
Definition
|
|
Term
| what color result in LIA test means that lysine has been decarboxylated? |
|
Definition
| purple slant, purple butt |
|
|
Term
| what color result in LIA test means that glucose was fermented? |
|
Definition
| purple slant, yellow butt |
|
|
Term
| what test is better for H2S production, TSI of LIA? |
|
Definition
|
|
Term
| what enzyme does oxidase test test for? what reagent? |
|
Definition
cytochrome oxidase
tetra methyle p-phenylenediamine dihydrochloride |
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