Term
|
Definition
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|
Term
| Phenol Red Broth Substrate |
|
Definition
| Glucose, sucrose, or lactose. |
|
|
Term
| Phenol Red Broth Indicator |
|
Definition
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|
Term
| Phenol Red is yellow below what pH and pink above what pH? |
|
Definition
| yellow below 6.8, pink above 7.4 |
|
|
Term
| Phenol Red Broth end product? |
|
Definition
|
|
Term
| SIM test (Sulfur) Substrate |
|
Definition
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Term
|
Definition
| cysteine desulfurase or thiosulfate reductase |
|
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Term
| SIM test (Sulfur) end product |
|
Definition
|
|
Term
| SIM test (Sulfur) byproduct |
|
Definition
| Hydrogen sulfide that combines with iron in the SIM medium to form ferric sulfide |
|
|
Term
| SIM test (Indole) substrate |
|
Definition
| tryptophan in casein and animal tissue. |
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Term
|
Definition
|
|
Term
| SIM test (Indole) end product |
|
Definition
|
|
Term
| SIM test (Indole) byproducts |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| Kovac's reagent on a BBL DrySlide |
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|
Term
|
Definition
|
|
Term
| Gelatinase Test Substrate |
|
Definition
|
|
Term
|
Definition
|
|
Term
| Gelatinase test End product |
|
Definition
| Gelatin is hydrolyzed by gelatinase into polypeptides which are hydrolyzed by gelatinase again into AMINO ACIDS. |
|
|
Term
|
Definition
|
|
Term
|
Definition
| Glucose, lactose, sucrose, and sulfur |
|
|
Term
|
Definition
| Enzymes for carbohydrate fermentation (sucrase, galactosidase) and thiosulfate reductase |
|
|
Term
|
Definition
| Pyruvate which is fermented and H2S |
|
|
Term
|
Definition
| Various products from fermentation of pyruvate |
|
|
Term
|
Definition
|
|
Term
| Methyl Red Test Substrate |
|
Definition
|
|
Term
|
Definition
| Enzymes needed for glycolysis and fermentation of pyruvate. |
|
|
Term
|
Definition
|
|
Term
| At what pH is methyl red red? At what pH is it yellow? |
|
Definition
Red: pH 4.4
Yellow: pH 6.2 |
|
|
Term
|
Definition
| Various products from fermentation of pyruvate |
|
|
Term
|
Definition
|
|
Term
| Voges-Proskauer substrate |
|
Definition
|
|
Term
|
Definition
| Enzymes needed for glycolysis and fermentation of pyruvate |
|
|
Term
| Voges-Proskauer indicator |
|
Definition
|
|
Term
| Voges -Proskauer end products |
|
Definition
| Acetoin which is then turned into diacetyl and 2,3-butanediol. |
|
|
Term
| Nitrate Reduction Test Medium |
|
Definition
|
|
Term
| Nitrate Reduction Test substrate |
|
Definition
|
|
Term
| Nitrate reduction test enzyme |
|
Definition
|
|
Term
| Nitrate reduction test indicator |
|
Definition
Positive: gas in Durham tube
Otherwise: sulfanilic acid added. Red=nitrate
If no color change: zinc added which catalyzes nitrate into nitrite and turns broth red=negative test because nitrate was not originally reduced.
No color change: positive because the nitrate was reduced to something else. |
|
|
Term
| Nitrate reduction test end products |
|
Definition
| Positive test: nitrite, ammonia, nitric oxide, and nitrous oxide. |
|
|
Term
| Phenylalanine Deaminase Test medium |
|
Definition
|
|
Term
| Phenylalanine Deaminase Test substrate |
|
Definition
|
|
Term
| Phenylalanine Deaminase Test enzyme |
|
Definition
|
|
Term
| Phenylalanine Deaminase Test Indicator |
|
Definition
| Ferric chloride, which turns green in the presence of phenylpyruvic acid |
|
|
Term
| Phenylalanine Deaminase Test end product |
|
Definition
|
|
Term
| Phenylalanine Deaminase Test by product |
|
Definition
|
|
Term
| Starch Hydrolysis Test Medium |
|
Definition
|
|
Term
| Starch Hydrolysis Test Substrate |
|
Definition
|
|
Term
| Starch Hydrolysis Test Enzyme |
|
Definition
| a-amylase or oligo-1,6 glucosidase |
|
|
Term
| Starch Hydrolysis Test Indicator |
|
Definition
| Iodine. A positive will have a clear halo around the inoculation. |
|
|
Term
| Starch Hydrolysis Test end product |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
| Catalase Test End Products |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
| At what pH is Bromthymol blue green? At what pH is it blue? |
|
Definition
Green: pH 6.9
Blue: pH 7.6 |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| ammonia and ammonium hydroxide which alkalinize the agar and turn it blue, a positive result. |
|
|
Term
|
Definition
|
|
Term
|
Definition
| Tetramethyl phenylenediamine |
|
|
Term
|
Definition
|
|
Term
|
Definition
| Cytochrome c oxidase rapidly oxidizes tetramethyl phenylenediamine which turns it purple. |
|
|
Term
| Mannitol Salt Agar Test Medium |
|
Definition
|
|
Term
| Mannitol Salt Agar Test Substrate |
|
Definition
|
|
Term
| Mannitol salt agar test indicator |
|
Definition
|
|
Term
| Mannitol Salt Agar Test Enzyme |
|
Definition
|
|
Term
| MacConkey Agar Test Medium |
|
Definition
|
|
Term
| MacConkey Agar Test Substrate |
|
Definition
|
|
Term
| MacConkey Agar Test Indicator |
|
Definition
|
|
Term
| At what pH is neutral red colorless? At what pH is it red? |
|
Definition
Colorless: above pH 6.8
Red: below pH 6.8 |
|
|
Term
| MacConkey Agar Test Enzyme |
|
Definition
|
|
Term
| Eosin Methylene Blue Agar Test Medium |
|
Definition
|
|
Term
| Eosin Methylene Blue Agar Test Substrate |
|
Definition
|
|
Term
| Eosin Methylene Blue Agar Test Indicators |
|
Definition
| Eosin Y and methylene blue |
|
|
Term
| Eosin Methylene Blue Agar Test Enzyme |
|
Definition
|
|
