Term
| What does ELISA short for? |
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Definition
| Enzyme-Linked Immunosorbent Assay |
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Term
| What is another name for mononucleosis? |
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Definition
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Term
| Why is it called the kissing disease? |
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Definition
| It is believed that kissing is the mode of transmission. |
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Term
| What is another mode of transmission? |
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Definition
| Any oral direct contact with infected saliva (straws, pop bottle) |
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Term
| What is the virus that causes mononucleosis? |
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Definition
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Term
| What is the name for infections without symptoms? |
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Definition
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Term
| What is the name of infections that are fast onset? |
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Definition
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Term
| What are the characteristics of EBV infections and the mononucleosis disease? |
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Definition
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Term
| What are other diseases caused by EBV? |
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Definition
Burkitt's Lymphoma Nasopharyngeal Carcinoma AIDS Chronic Fatique Syndrome
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Term
| What is the common denominator of all EBV diseases? |
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Definition
| Elevated antibody titers (levels) usually IgG. |
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Term
| What is another name for Chronic Fatigue Syndrome caused by EBV? |
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Definition
| Yuppy or Generation X disease because of the major symptom of fatigue |
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Term
| What are the two ways to identify EBV infections? |
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Definition
ELISA - measure elevated Abs PCR (Polymerase Chain Reaction) - detection of viral DNA
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Term
| What is the basic setup for the ELISA test? |
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Definition
Testing of blood (synthetic) serum Four students (Suzy, Mary, Carol and Irene) who are suite mates complaing of fatigue. Two controls (positive and negative)
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Term
| What are the two results of the ELISA test? |
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Definition
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Term
| Generally describe the ELISA test procdure? |
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Definition
Ab is produced in IR to Ag (drawn in blood serum) Ag is added to well and coats. Patients Ab is added and binds to Ag (not visible) Secondary Ab with bound enzyme is added to well and is specific to EBV Ab (will not bind to Ag if EBV is not present) PBS is a buffer solution used to wash/rinse unbound EBV Ab and Secondary Ab out of well. Substrate with dye binds to Enzyme on Secondary Ab only if present.
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Term
| What is the purpose of the negative control? |
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Definition
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Term
| What was the purpose of the positive control? |
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Definition
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Term
| What does mononucleosis occur in young people in the US and the rest of the world at a younger age? |
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Definition
Poor sanitation and inadequate hygiene leads to younger peoples infections Younger people have immature IS, specifically the TC (cytotoxic) cells, that cannot cause severe tissue damage typical of mono symptoms.
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