Term
| What is the specific function of Nitrogen Fixers? |
|
Definition
| To reduce atmospheric N2 gas into usable nitrogen forms |
|
|
Term
| Where might you find a symbiotic nitrogen fixer? |
|
Definition
| In the soil, on a root nodule of a plant such as a legume |
|
|
Term
| The Winogradsky Column is used to demonstrate what cycle? |
|
Definition
|
|
Term
| What is found in the Black Zone of the Winogradsky Column? |
|
Definition
| Anaerobic Chemoorganoheterotrophs |
|
|
Term
| What is found in the top level (water) of the Winogradsky Column? |
|
Definition
| Oxygenic Photolithoautotrophs (Cyanobacteria) |
|
|
Term
| What is found in the Red and Green Zones (Below the Rust Zone) of the Winogradsky Column? |
|
Definition
| Anoxygenic Photolithoautotrophs (Purple and Green Sulfur Bacteria) |
|
|
Term
| What is found in the Rust Colored Zone of the Winogradsky Column? |
|
Definition
| Anoxygenic Photoorganoheterotrophs |
|
|
Term
| What is found in the oxygen dominated much (Just below the water) of the Winogradsky Column? |
|
Definition
| Aerobic Chemolithoautotrophs |
|
|
Term
| The lights shining on the Winogradsky Column are to benefit what population? |
|
Definition
|
|
Term
| Name the three types of media used in the Soil Lab |
|
Definition
1. Soil Plate
2. ACT-Plate (Actinomyces Isolation Agar)
3. Rhizobium Plate |
|
|
Term
| What are the two categories of Microbes founds in the soil? |
|
Definition
1. Spore Formers (Bacillus)
2. Non-Spore Formers (Actinomyces) |
|
|
Term
| What is the equation for Nitrogen Fixation? |
|
Definition
|
|
Term
|
Definition
|
|
Term
| What are the two selective ingredients of the Soil Extract Agar? |
|
Definition
1. Cyclohexamide
2. 10% Soil Extract |
|
|
Term
| What does Cyclohexamide SELECT for and INHIBIT? |
|
Definition
Selects for -> PROCARYOTES
Inhibits -> EUCARYOTES |
|
|
Term
| What does 10% Soil Extract SELECT for? |
|
Definition
| Specific Soil Microbes (Procaryotes) |
|
|
Term
| What does 10% Soil Extract INHIBIT? |
|
Definition
| Non Specific Soil Microbes |
|
|
Term
| What does SOIL EXTRACT AGAR select for? |
|
Definition
| Specific Soil Procaryotes |
|
|
Term
| What does the ACT Plate select for? |
|
Definition
|
|
Term
| What are the 3 selective ingredients in the ACT Plate? |
|
Definition
1. Blood
2. Metrodinazole
3. Nalidixic Acid |
|
|
Term
| What does the RHIZ (Rhizobium) Plate select for? |
|
Definition
|
|
Term
| What are the two selective ingredients in Rhizobium Agar and what do they select for? |
|
Definition
1. Yeast Extract -> Source of organic nitrogen
2. Mannitol -> Carbon Source in growing media |
|
|
Term
| What is the purpose of Rhizobium Agar? |
|
Definition
| Try to culture Rhizobium from a clover root. |
|
|
Term
| What is Rhizobium? (4 things) |
|
Definition
1. Symbiotic Nitrogen Fixer
2. Gram Negative
3. Can fix nitrogen while symbiotically infecting Legumes
4. When fixing nitrogen, Rhizobium looks like a bacteroid |
|
|
Term
|
Definition
| A BACTEROID changes morphology to fix Nitrogen |
|
|
Term
|
Definition
| E. Coli produces an ENZYME, B-Glucoronidase, which will act on the MUG converting it to a fluorescent compound that will fluoresce under a UV Light |
|
|
Term
|
Definition
| 4-Methylumbelliferone Glucuronide |
|
|
Term
| Why do we use TSA plates AND Soil Extract Agar plates in the Soil Lab? |
|
Definition
We use SOIL EXTRACT because it selects for soil specific procaryotes.
We use TSA because it selects for procaryotes AND eucaryotes |
|
|
Term
| What is the starting material in the SOIL LAB? (What would the units be?) |
|
Definition
| Soil therefore measured in GRAMS! |
|
|
Term
| What are the units in the soil lab? |
|
Definition
|
|
