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Definition
| study of the diversity of organisms and their relationships |
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| What does systematics link? |
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| the science of biological classification |
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| determining that a particular isolate belongs to a recognized taxon |
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| assignment of names to the taxonomic group |
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| What did taxonomy used to rely on? |
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| Now what approach is used in taxonomy? |
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| What are the three parts of polyphasic |
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Definition
Phenotypic Genotypic Phylogenetic |
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| What are the five parts of phenotypic analysis? |
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Definition
| Motility, Morphology, Metabolism, Physiology, and Cell Chemistry |
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| colony morphology, Gram reaction, cell size and shape |
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| Nonmotile, gliding, swimming, swarming motility |
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| Utilization of C, N2 or S compounds, fermentation of sugars, N2 fixation |
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| Temperature, pH and salt ranges for growth, response to O2, production of extracellular enzymes |
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Definition
| Fatty acids, polar lipids |
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Definition
| FAME fatty acid methyl ester |
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Definition
| variation in type and proportion of fatty acids present in membrane lipids |
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| Why does FAME require rigid standardization? |
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Definition
| because FAME profiles can vary as a function of temperature, growth phase, and growth medium |
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| What does genotypic analysis provide? |
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Definition
| Information at the DNA level |
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| What are the four types of genotypic analysis? |
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Definition
DNA-DNA hybridization DNA profiling methods Multilocus Profiling GC ratios |
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Definition
| focuses on identifying species within a genus |
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Definition
| Focuses on identifying strains within a species |
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| Focuses on multiple genes |
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| Percentage of guanine plus cytosine bp in the genome |
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complation of both the phenotypic and genotypic info Based on analysis of Individual genes – 16S rRNA Multiple genes Whole genome |
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| What percent is the same species when comparing DNA-DNA hybridization |
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Definition
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| What percent is the same species in 16s RNA gene sequence identity |
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Definition
| The International Code of Nomenclature of Bacteria |
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| Taxonomic outline of the prokaryotes |
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Definition
| Second edition of Bergey’s Manual of Systematic Bacteriology |
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Definition
| compendium of information on all recognized species of bacteria |
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Definition
| provides detailed information on the enrichment, isolation, and culture of the many groups of Bacteria and Archaea |
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