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Microbiology Exam 1
lab tests, definitions, etc.
38
Microbiology
Undergraduate 1
09/12/2013

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Cards

Term
Normal Flora
Definition
What you carry on your skin or inside you that does NOT cause any disease
Term
4 Major Types of Culture Media
Definition
Agar Slant
Agar Plate
Broth
Deep
Term
Transient Flora
Definition
Bacteria you pick up from your environment; Bacteria that gets where it's not supposed to
Term
Subculture
Definition
Procedure that helps to verify the purity of that colony.
Contain only ONE bacteria type.
Term
Sterilization
Definition
Kills ALL bacteria so there's nothing left
Term
Disinfection
Definition
Reduce the number of bacteria on a surface
Term
Denaturation
Definition
Alter protein structure to kill bacteria
Term
What is the most effective of all the physical means of controlling organisms?
Definition
Heat
Term
Thermal Death Point
Definition
The lowest temperature required to sterilize a standard quantity of pure culture within 10 minutes.
Term
Thermal Death Time
Definition
The time required to sterilize the culture at a particular temperature.
Term
Autoclave Settings
Definition
121 degrees C
15 psi
15 minutes
Term
Antibiotic
Definition
Chemical produced by a bacteria that kills or inhibits the growth of another bacteria
Term
Static
Definition
To stop growth of bacteria
Term
Cidal
Definition
To kill bacteria
Term
Disinfectant
Definition
Chemical you use on inanimate objects to kill or reduce the number of bacteria on an object
Term
Antiseptic
Definition
Chemical that can be safely used on living tissue
Term
Kirby-Bauer Method
Definition
Utilizes paper disks impregnated with an antibiotic of specific concentration
Term
3 Kirby-Bauer Method Measurements
Definition
Resistant (won't work)
Sensitive (will work)
Intermediate (50/50 chance of success)
Term
Ocular
Definition
The lens that fits into upper portion of the body tube to view the specimen.
10X magnification
Term
Revolving Nosepiece
Definition
The lower portion of the body tube supporting 2-4 objectives.
Rotates objectives into position.
Term
Objectives
Definition
Formation and magnification of the image
Term
Mechanical Stage
Definition
Supports slide
Moves slide around
Term
Arm
Definition
Part of the supportive structure
Term
Condenser
Definition
Concentrates light onto the slide
Term
Coarse Adjustment-Knob
Definition
Moves object the greater distance
Term
Fine Adjustment-Knob
Definition
Brings specimen into clearest focus
Term
Iris Diaphragm Lever
Definition
Adjust amount of light passing through specimen
Term
Base
Definition
Supportive structure at bottom of microscope
Term
Parfocal Microscope
Definition
You can switch from one objective to another without having to refocus your lens.
Term
Field of View
Definition
Any and everything you see through the microscope lens
Term
Mechanical Stage Knob
Definition
Moves slide left/right and up/down
Term
4 Objective Lenses on Parfocal Microscope
Definition
RED-Scanning: 4x
YELLOW-Low: 10x
BLUE-High: 40x
WHITE-Oil Immersion: 100x
Term
How to figure TOTAL magnification
Definition
10 X OBJECTIVE LENS MAGNIFICATION
Term
3 Types of Physical Control
Definition
Heat
Radiation
Filtration
Term
2 Types of Chemical Control
Definition
Disinfectants
Antiseptics
Term
Aseptic Technique
Definition
Practiced by using techniques to prevent contaminating your cultures, yourself, your lab partner, and your work station.
Term
Give an Example of DRY HEAT
Definition
Fire
Incineration
Flames
Term
Give an Example of MOIST HEAT
Definition
Autoclave Machine
Boiling
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