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Microbiology Chapter 9
Set 2
31
Other
Undergraduate 2
03/07/2009

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Term
Metabolism
Definition

all chemical reactions and physical workings of the cell

3 functions- breaks down molecules

builds molecules

stores ATP for energy

Term

The metabolism of microbes

1.Anabolism

VS

2.Catabolism

Definition

1. (biosynthesis)  building of moleculesany process that results in synthesis of cell molecules and structures-usually requires energy input

2.the breakdown of bonds of larger molecules into smaller molecules (often releases energy)

Term

Enzymes

Definition
biological catalyst chemicals that increase the rate of a chemical reaction without becoming a part of the products. They are required for life because w/o them reactions would not be fast enough
Term
Energy of activation
Definition
The amount of energy that must be overcome before a reaction can begin
Term
What are 3 ways enough energy can be overcome to begin a reaction?
Definition

1. increasing thermal energy to increase molecular velocity

2.increasing the concentration of reactant to increase the amount of collisions

3.Adding a catalyst (only living organisms)

Term
What is the stucture of an enzyme?
Definition
Mostly protein w/  primary, secondary, tertiary and quaterney levels of molecular complexity
Term
simple enzyme
Definition
consist only of a protein
Term
conjugated/halonzyme
Definition

contains protein and non protein molecules

a apoenzyme and 1 or more cofactors which can be organic or inorganic

Term
Metallic cofactors
Definition

Fe, Cu, Mg,Mn, Zn, Co,Se

they activate binding sites by attracting the substrate closer

Term
coenzymes
Definition
organic enzymes that work in conjugation with an apoenzyme to form alteration of a substrate it works by removing a substrate molcule from one substrate to another. The vitamin is one of the most important components of a coenzyme.
Term
Active Site
Definition
the actual site where the substate binds to the active site
Term
Exoenzymes
Definition
breakdown molecules outside of the cell
Term

Endoenzymes

Definition
break down molecules inside of the cell
Term

Rate of enzyme production

1.Constitutive enzymes

Vs

2.Regulated enzmes

 

Definition

1.always present in relatively constant amounts

2. production is either induced or repressed in a responce to a change in concentration need

Term
Dehydration Reaction
Definition

Synthesis of polymers requiring ATP-

release water

Term
Hydrolysis Reaction
Definition
breaks down substrates using water
Term
What are 3 operating conditions that effect the production of enzymes?
Definition

1.temperature

2.pH

3.osmotic pressure

 

Term
Denaturation
Definition

the weak bonds that maintain shape are broken

-protiens

Term

Metabolic Pathways

1.  linear pathways

Vs.

2. cyclic pathways

Definition

1. product of one reaction serves the substrate for the next.

2. starting molecule is regenerated to initiciate another turn of the cycle

Term

Regulation of enzyme productivity

1. competive inhibition

Vs.

2. noncompetitive inhibition

Definition

1.a molecule that simulates the direct size of the substrate, then blocks the active site to prevent reactions

2. binds to a secondary site which alters the shape and chemical makeup of the cell so the substrae could not bind

Term

Enzyme Production

1. Enzyme repression

Vs

2. Enzyme induction

Definition

1. the anabolic pathway is turned off due to an increased amount

2.the substrate is present and the catabolic pathway is turned on

Term
Adenosine Triphosphate
Definition

nitrogen base, with a 5 carbon sugar, chain of negatively charged phosphate groups

 

carries high amount of energy in the bonds

Term
glycolysis
Definition

metabolic pathway used by most organisms to break down glucose

4 ATP-----2 used 2 left over ----net 2 ATP

Glucose------2 pyruvate

2 NAD+-----NADH

Glucose + 2 NAD+ + 2 Pi + 2 ADP → 2 pyruvate + 2 NADH + 2 ATP +2H+ + 2 H2O

Term
Aerobic Respiration
Definition

2 pyruvate----2 Acetyl CoA

-----2 CO2

2 NAD+----NADH

Term
Krebs Cycle
Definition

Acetyl CoA combines W/oxaloacetate------citric acid

2 ATP's needed to begin

yields-2 Acetyl Co-as which gives 2 cycle

 

Term
Total yield or aerobic respiration
Definition

38 ATP

6CO2

6H2O

Term

1anaerobic respiration

VS

2aerobic respirtation

Definition

1more productive, uses free oxygen

2.uses oxygen containing ions

Term
Fermentation
Definition

2 ATP

recycles NADH back into NAD+

Term
What are the products of fermentation?
Definition

alcoholic beverages

Dairy products

Vitamins

organic acids

 

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