Term
| Define Spontaneous Generation |
|
Definition
| The theory that organisms arise spontaneously from non-living material |
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|
Term
| What is the scientific contribution of Hooke? |
|
Definition
| Hooke saw microobes in 1665 |
|
|
Term
| What is the scientific contribution of Leeuwenhoek? |
|
Definition
| Leeuwekhoek saw bread mould in 1674 |
|
|
Term
| What is the scientific contribution of Pasteur? |
|
Definition
| Pasteur disproved spontaneous generation in 1861 |
|
|
Term
| What is the scientific contribution of Tyndall? |
|
Definition
| Tyndall discovered endospores in the 1860's. |
|
|
Term
| What is the scientific contribution of Jenner? |
|
Definition
| Developed smallpox vaccine in 1796 |
|
|
Term
| What is the scientific contribution of Koch? |
|
Definition
| Developed protocols for handling bacteria in lab, established rules to be used to determine causative agent of disease (Koch’s Postulates) in the 1800s |
|
|
Term
| What is the scientific contribution of Ehrlich? |
|
Definition
| Started chemotherapy to treat diseases in the 1900s |
|
|
Term
| What is the scientific contribution of Fleming? |
|
Definition
| Discovered Penicillin in the 1900s |
|
|
Term
| How do the three Domains differ in size? |
|
Definition
| Bacteria (0.3-2µm) Archaea (0.3-2µm) Eucarya (5-50µm) |
|
|
Term
| Which domains have nuclear membranes? |
|
Definition
|
|
Term
| Which domains have peptides in the cell wall? |
|
Definition
|
|
Term
| Which domains have membranous organelles? |
|
Definition
|
|
Term
| Which domains are prokaryotes? |
|
Definition
|
|
Term
|
Definition
| Single celled organism with no membrane bound nucleus or lipid bound organelles with DNA in a nucleoid |
|
|
Term
| List the organization of biological hierarchy, from greatest to least specificity |
|
Definition
| DKPCOFGS (Don’t Kick Police Cars, Otherwise Fools Get Shot) - Domain Kingdom Phylum Chordata Order Family Genus Species |
|
|
Term
|
Definition
| Diverse single and multi celled organisms that have chloroplasts, undergo photosynthesis, have flagella and are found primarily in water |
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|
Term
|
Definition
| Single and multi celled organisms that gain energy from degrading organic material and are found primarily on land |
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|
Term
|
Definition
| Single celled organisms with un-rigif cell walls that are found on both land and water and require organic compounds as food which they ingest as particles. |
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Term
|
Definition
| Nucelic acid with a protein coat, that can only multiply inside a host and is therefore an obligate intracellular parasite |
|
|
Term
|
Definition
| An organism that consist of short RNA segment without a protein coat that can only multiply inside a host |
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|
Term
|
Definition
| An organism consisting of protein without nucelic acid |
|
|
Term
|
Definition
| A parasite such as round and tape worms |
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|
Term
| How do viruses, prokaryotes and eukaryotes differ in size? |
|
Definition
| Viruses (10nm-100nm) Prokaryotes (100nm-1000nm) Eukaryotes (10m-10mm) |
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|
Term
| What 6 elements make up 99.5% of living material by weight? |
|
Definition
|
|
Term
|
Definition
| 2 or more atoms with a chemical bond |
|
|
Term
|
Definition
| 2 or more different elements bonded together |
|
|
Term
| How are ionic bonds created? |
|
Definition
| Two or more elements trade electrons, join as ions |
|
|
Term
| How do ionic bonds fare in water? |
|
Definition
| Break at room temperature, 100x weaker than covalent |
|
|
Term
| What defines an organic compound? |
|
Definition
| Covalent bonds with C and H |
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|
Term
| How do Polar and Non-polar covalent bonds differ? |
|
Definition
| Polar have slight pos. and neg. charges (OH/NH/OC/NC) versus no charges (CC/CH/HH) |
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|
Term
| How are Hydrogen bonds formed? How strong are they? |
|
Definition
| H+ attracted to negatively charged atom. Weak, constaltly breaking and forming at room temperature |
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|
Term
| Why is water considered a universal solvent? |
|
Definition
| Dissolves polar compounds - splits them into ions (polar sides attract - ion, etc) |
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|
Term
| What is the concentration of OH and H ions in solutions that are Basic, Acidic, and Neutral? |
|
Definition
| Basic (10[OH-], 10^14[H+]), Acidic (10^7[OH-], 10^7[H+]) , Neutral (10^14[OH-], 10^0[H+]) |
|
|
Term
| What is the function of proteins? |
|
Definition
| Catalyze enzyme reactions, determine structure/shape of ribosomes, take nutrients in/out of cell |
|
|
Term
| What is the general structure of an amino acid, and how many are there? |
|
Definition
| Amino + side chain + carboxyl. 20 different ones that differ by side chain |
|
|
Term
| How do hydrophobic and philic proteins differ? |
|
Definition
| Phobic - nonpolar, many CH3 groups. Philic - polar, few CH3 groups |
|
|
Term
| What is the function of carbohydrates? |
|
Definition
| Food, building block of DNA |
|
|
Term
| What is the ratio of CHO in carbohydrates? |
|
Definition
|
|
Term
| What are the two types of monosaccharides and how do they differ? |
|
Definition
| 5-carbon (Deoxy/ribose), 6-carbon (gluocose, galactose, fructose) |
|
|
Term
| What are 2 examples of disaccarides? |
|
Definition
|
|
Term
| What are 2 examples of polysaccarides? |
|
Definition
|
|
Term
|
Definition
| Polymer containing 3-6 monosaccarides |
|
|
Term
| What is the structure of DNA |
|
Definition
| N-ring base (purine) with deoxy base and a phosphate backbone, wound around histones |
|
|
Term
| What is the structure of RNA |
|
Definition
| Single stranded DNA with ribose base and U instead of T |
|
|
Term
| Are lipids hydrophilic or hydrophobic? |
|
Definition
|
|
Term
| How do simple lipids differ from carbohydrates? |
|
Definition
|
|
Term
| What are Fats composed of? |
|
Definition
|
|
Term
| How can you determmine if a fat is a mono/di/triglyceride? |
|
Definition
| Count the number of fatty acid chains |
|
|
Term
| In what case is a simple lipid unsaturated? |
|
Definition
| Double bond s are present in a fatty acid chain (liquid at room temperature) |
|
|
Term
| What is the structure of compound lipids? |
|
Definition
| Fatty acids + glycerol + other |
|
|
Term
| What is the structure of Phospolipids? |
|
Definition
| Phosphate + fatty acid + glycerol |
|
|
Term
| What is the maximum magnification of a light microscope? |
|
Definition
|
|
Term
| How does light go from the lamp to eyes in a light microscope? |
|
Definition
| Specimen, magnifying lens |
|
|
Term
| What Domains are viewable under a light microscope? |
|
Definition
| Eucarya and Bacteria - good for up to 10µm |
|
|
Term
| What is the maximum magnification of an electron microscope? |
|
Definition
|
|
Term
| What acellular organisms are viewable under an electron microscope? |
|
Definition
|
|
Term
| What are the TEM and SEM used for viewing in a cell? |
|
Definition
| TEM (internal cellular structures), SEM (surface structures) |
|
|
Term
| What is the purpose of an atomic force microscope? |
|
Definition
| View objects at the atomic level |
|
|
Term
| How are magnification and resolution different? Which would provide better resolution - an electron or light microscope? |
|
Definition
Magnification is image enlargement, Resolution is the degree to which fine detail can be distinguished.
Light microscope will provide better resolution. |
|
|
Term
| How does a simple stain work? |
|
Definition
| Basic dye for color, negatively charged nucleic acids and proteins are stained with a positively charged dye |
|
|
Term
| What is a differential stain used for? |
|
Definition
| Distinguishing between bacterial groups |
|
|
Term
| What function does a gram stain serve? |
|
Definition
Determine if a cell is gram + or -, which reflect differences in cell wall structure.
1st stain - Purple
2nd stain - Pink |
|
|
Term
| What genus would you be trying to detect by using an acid-fast stain? |
|
Definition
|
|
Term
| Why is a capsule stain used? |
|
Definition
| Capsules don’t take up certain stains, this used to make capsules stand out against background |
|
|
Term
| Why is an endospore stain used? |
|
Definition
| Stains endospores, which do not stain easily |
|
|
Term
| Why is a flagella stain used? |
|
Definition
| Coats thin flagella, visible under light microscope |
|
|
Term
| What are the steps in a Gram stain? |
|
Definition
| Flood smear with primary stain, rince, flood with dilute iodine, rince, add 95% alcohol to decolor gram negative, counterstain added to stain gram negative |
|
|
Term
| What are the 4 bacterial shapes? |
|
Definition
| Cocci (spherical), Bactillus (columnar), Spiral, Pleomorphic (varying in shape) |
|
|
Term
| What is the function of the cytoplasmic membrane? |
|
Definition
| Selectively permeable barrier made of phospholipids with embedded sterols and hopanoids. |
|
|
Term
| What is the fluid mosaic model? |
|
Definition
| Proteins are not static, dynamic nature means the cytoplasmic membrane is constantly changing and fluid |
|
|
Term
| What is the structure of peptidoglycan? |
|
Definition
| AA/sugar mesh with covalent bonds, alternating NAG/NAM sugar units cross linked to AA's |
|
|
Term
| Peptidoglycan, found only in the cell walls, is the target of... |
|
Definition
| Antibiotics and Lysosome (a product of the body) |
|
|
Term
| What is the structure and function of the membrane outsode of the peptidoglycan layer in Gram Negative bacteria? |
|
Definition
| Consists of LPS (endotoxin), phospholipids and proteins, plays a role in fever/inflammation |
|
|
Term
| How do capsule and slime layers differ? What is their function? |
|
Definition
Gel like layers that function as protection/attachment. Capsule layer (distinct/gelatinous) Slime Layer (diffuse/irregular)
Function in adhereing to surfaces are thwarting innate defense system |
|
|
Term
| What is the function of flagella? |
|
Definition
| Protein structure responsible for mobility, spin in propellar like fashion |
|
|
Term
|
Definition
| Phenomenon where bacteria sense and move according to chemical attractants (cells move towards) or repellants (cells move away) |
|
|
Term
| What is the function of pili and how do they differ from flagella? |
|
Definition
| Attachment to surfaces/bacterial mating. Shorter/thinner than flagella and can't been seen under light microscope |
|
|
Term
|
Definition
| Circular, supercoiled double stranded DNA that the cell does not actually need, but may provide an advantage (can encode enzymes that destroy antibiotics) |
|
|
Term
| How are bacterial ribosomes different from eukaryotic ones? Why is this useful? |
|
Definition
| Size/composition. Exploited for drug design |
|
|
Term
| What are storage granules? |
|
Definition
| Polymers of nutrients stored until required |
|
|
Term
| How are glucose, carbon and phosphate stored as granules? |
|
Definition
| Glycose (glycogen), Carbon (Poly-B-hydroxlbutrate), Phosphate (Volutin granules) |
|
|
Term
| What is an endospore and why is it advantageous? |
|
Definition
| A resistant form of life some bacteria (particularily Bactiuus/Clostridium) can differentiate to, advantageous as it allows cells to lie dormant until conditions improve |
|
|
Term
|
Definition
| DNA surrounded by many levels of cytoplasmic membtrane/peptidoglycan. Can't reproduce or grow, will revert when conditions improve |
|
|
Term
|
Definition
| Long hollow cylinder form mitotic spindle, make up cilia and flagella framework along which organelles move |
|
|
Term
|
Definition
|
|
Term
| Describe intermediate filaments |
|
Definition
| Function like ropes, strengthening cell mechanically, enable cell to resist physical stresses |
|
|
Term
| How are flagella different/simmilar? |
|
Definition
| Protein appendages that appear to project out but are covered by cytoplasmic membrane extension, flagella give mobility, cilia help move material away/towards cell |
|
|
Term
| Describe the structure of the nuclear envelope |
|
Definition
| Two lipid bilayer membranes, protein strucures (nuclear pores) |
|
|
Term
| Where is mRNA synthesized? |
|
Definition
|
|
Term
| Describe the structure of the mitochondria membrane |
|
Definition
| Two lipid bilayer membranes (inner/outer), inner highly folded (cristae) to increase surface area, enclosed by inner is the matrix |
|
|
Term
| What is the stroma in chloroplasts analgous to in mitochondria? |
|
Definition
|
|
Term
| Define generation/doubling time |
|
Definition
| The time for a population to double in number (generally bacteria) |
|
|
Term
| What do the variables in the function: Nt = N0 x 2n represent |
|
Definition
| N0 - original number of cells, Nt - number of cells at given time t, n - number of divisins during time t |
|
|
Term
|
Definition
|
|
Term
| Define Aseptic techniques |
|
Definition
| procedures that minimize chance of organisms being introduced |
|
|
Term
|
Definition
| Solidifying a liquid culture medium |
|
|
Term
| Describe the streak-plate method |
|
Definition
| Sterilized loop dipped in bacteria, and drawn across a plate three times at different angles to dilute the concentration of cells |
|
|
Term
|
Definition
| A culture stored for later use |
|
|
Term
| Define a closed/batch culture |
|
Definition
| A culture in which nutrientsare not renewed and wastes are not removed |
|
|
Term
| Define an open/continuous culture |
|
Definition
| A culture in which nutrients are added and wastes removed regularily |
|
|
Term
| What are the phases in bacterial growth in a culture? |
|
Definition
| Lag, exponential (log), stationary, death, prolonged death (LESDP) |
|
|
Term
|
Definition
| Bacterial culture diluted/transferred, cells not rapidly increasing for the moment (synthesizing macromolecules) |
|
|
Term
| Describe the exponential (log) phase |
|
Definition
| Cells divide at a constant rate, generation time measured, primary and secondary metabolites synthesized in early/late log phase. Endospores form in late log phase if possible |
|
|
Term
| Describe the stationary phase |
|
Definition
| No more energy or nutrients, number of cells constant because some die and release contents and are cannabalized |
|
|
Term
|
Definition
| Decrease in number of viable cells, exponential but slower than growth |
|
|
Term
| Described the prolonged death phase |
|
Definition
| 99% dead, days to years, cannibalizing continues, survival of the fittest |
|
|
Term
| Define optimum growth temperature |
|
Definition
| Temperature at which growth is fastest |
|
|
Term
|
Definition
|
|
Term
|
Definition
| OGT 20-30, cause food spoilage |
|
|
Term
|
Definition
| OGT 35-40, found in human body - most common pathogens |
|
|
Term
|
Definition
| OGT 45-70, hot springs/compost/water heaters |
|
|
Term
|
Definition
|
|
Term
|
Definition
| Absolute oxygen requirements |
|
|
Term
| Define obligate anaerobes |
|
Definition
| Capable of growth in the absence of oxygen |
|
|
Term
| Define facultative anaerobes |
|
Definition
| Growth better with oxygen |
|
|
Term
|
Definition
| Small oxygen requirements, more inhibitory |
|
|
Term
| Define aerotolerant anaerobes |
|
Definition
|
|
Term
| Why do all cells attempt tp maintain a cytoplasmic pH near 7? |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| Prefer pH 8.5 or higher (exchange internal Na ions for internal protons) |
|
|
Term
|
Definition
| Solute concentration higher out of cell - cell dehydrates |
|
|
Term
|
Definition
| Tolerate high salt concentrations |
|
|
Term
|
Definition
| Require high salt concentrations |
|
|
Term
| What is the function of the cell wall in maintaining water homeostasis? |
|
Definition
| Enables bacteria to survive hypotonic enviornment while preventing osomtic lysis |
|
|
Term
| How do cells prevent plasmolysis |
|
Definition
| Increase internal solute concentration |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| Use energy from oxidizing chemical compounds |
|
|
Term
|
Definition
| Contains variety of ingredients |
|
|
Term
|
Definition
| Contains precise amounts of pure chemicals |
|
|
Term
| Define differential media |
|
Definition
| Contains substances that bacteria change in a recognizable way |
|
|
Term
|
Definition
| Includes total number of cells (living and dead) |
|
|
Term
|
Definition
| Includes viable cells, 24 hour incubation required |
|
|
Term
| Describe the process of doing a plate count |
|
Definition
| Sample diluted in 10 fold increments, 0.1-1mL transferred to sterile petri dish, mixed with agar, when agar hardens - plate incubated and colonies farm (multiple layers) |
|
|
Term
| Describe the process of doing a spread plate |
|
Definition
| Plate count where 0.1-0.2 mL dilution spread on hard agar plate with sterile glass rod, after incubation colonies form on surface (1 layer) |
|
|
Term
|
Definition
| Process of destroying all micro-organisms/viruses through physical/chemical means |
|
|
Term
|
Definition
| Process that eliminates most/all pathogens in the material |
|
|
Term
|
Definition
| Disinfectant non-toxic enough to be used on skin |
|
|
Term
|
Definition
| Process that substantially reduces microbe population to meet health standards |
|
|
Term
| Does Pasteurization sterilize? |
|
Definition
| No, pasteurization reduces the number of organisms |
|
|
Term
| What is the HTST form of Pasteurization? |
|
Definition
| High temperature, low time - 72 degrees for 15 seconds |
|
|
Term
| What is the UHT form of Pasteurization? |
|
Definition
| Ultra high temperature (sterilizes) 140 degreece for 12 seconds |
|
|
Term
| What does an autoclave do? |
|
Definition
| Sanitizes and kills endospores - pressurized steam at 121 degrees at 15 psi for 15 minutes |
|
|
Term
| How does canning work, and what is the process for home and commercial canning? |
|
Definition
| Moist heat/Anaerobic Chamber. Home (115 @15 min) Commercial (121 @ 2.5 min) |
|
|
Term
| What do drying ovens do? Give an example of something sterilized by drying ovens |
|
Definition
| Oxidize cell components, denatures proteins, high temp/time required versus moist heat. Lab glassware (160@2-3 hours) |
|
|
Term
| Give some examples of physical methods of destroying organisms |
|
Definition
| Steriliants, high/intermediate/low level disinfectants |
|
|
Term
| Define steriliants and provide an example item that would require their use |
|
Definition
| Destroy all microbes (including endospores and viruses), 6-10 hours, used on scalpels |
|
|
Term
| Define High Level Disinfectants and provide an example item that would require their use |
|
Definition
| All viruses and vegetative organisms (not endospores), sterilants used for 30 minutes. Used on gastrointestinal endoscopes. |
|
|
Term
| Define Intermediate Level Disinfectants and provide an example item that would require their use |
|
Definition
| Destroy vegetative bacteria, most viruses (not endospores). Used on stethoscopes |
|
|
Term
| Define Low Level Disinfectants and provide an example item that would require their use |
|
Definition
| Destroys fungi, vegetative bacteria, enveloped viruses |
|
|
Term
| What are some considerations to take into account when selecting a disinfectant? |
|
Definition
| toxicity, activity in presence of organic matter, compatibility, residue, enviornmental risk, cost/availbility, storage/stability |
|
|
Term
| How do Alcohols work? What are they effective against, and provide an example |
|
Definition
| Damage membrane lipids. Effective Against: VB/F (~E/V). Example: Ethanol/Isopropanol Alcohol |
|
|
Term
| How do Aldehydes work? What are they effective against, and provide an example |
|
Definition
| Sterilants, inactivate proteins/nuc acids. Effective Against: M/V. Example: 2% Glutaradehyde |
|
|
Term
| How do Biguanides work? What are they effective against, and provide an example |
|
Definition
| Disruput cell membranes. Effective Against: VB/F, some V. Example: Chlorohexadine |
|
|
Term
| How do Volatile Chemicals work? What are they effective against, and provide an example |
|
Definition
| Gas sterilants for large items, damage proteins. Effective Against: M/V. Example: Ethylene Oxide |
|
|
Term
| How do Halogens work? What are they effective against, and provide an example |
|
Definition
| Disinfectant, oxidize proteins/cellular components. Effective Against: M/V. Example: Cl, Bleach, Iodine |
|
|
Term
| How do Metal Compounds work? What are they effective against, and provide an example |
|
Definition
| Affect protein function, toxic to humans in effective concentrations. Effective Against: M/V. Example: Silver Nitrate, Mercury, Zinc |
|
|
Term
| How do Oxidizing Agents work? What are they effective against, and provide an example |
|
Definition
| Oxidize cell components. Effective Against: Anaerobic Organisms. Example: Ozone, Hydrogen Peroxide |
|
|
Term
| How do Phenals work? What are they effective against, and provide an example |
|
Definition
| Destroy Cytoplasmic Membrane, denature proteins, disinfectant. Effective Against: Most VB. Example: Lysol, mouthwash, cleaning up blood on hospital floor |
|
|
Term
| How do QUATS work? What are they effective against, and provide an example |
|
Definition
| Detergent, destroy Cytoplasmic Membrane and denature proteins. Effective Against: VB, some V. Example: Detergents |
|
|
Term
| Name some methods of preserving perishable products |
|
Definition
| Low temperature (enzyme reactions slowed), addition of salt/sugar (dehydrate cells), dessication (remove water), lycophilization (freeze dry) |
|
|
Term
| How does anabolic metabolism work? What does it require? |
|
Definition
| Builds larger molecules from smaller ones (biosynthesis). Requires: energy source, B12, biotin, folic acid, Vitamin E |
|
|
Term
| What is anabolic metabolism required for? |
|
Definition
| Replicating working molecules in cell, growth/division, secretion of cell products |
|
|
Term
| Explain how anabolic/catabolic pathways and ATP/ADP fit together to form a cycle |
|
Definition
| ADP is filled up with energy by enzymes in catabolic pathways to form ATP which is then broken down in anabolic pathways to form ADP |
|
|
Term
| What are the products and reactants of glycolysis? What is required for glycolysis? |
|
Definition
Reactants: glucose
Product: Pyruvic Acid. Enzyme niacin required |
|
|
Term
| Bacterial break down pyruvic acid to _____ and _____ |
|
Definition
|
|
Term
| During fermentation, how many molecules (approx.) of ATP are gained for every glucose? Why is fermentation not efficient? |
|
Definition
36-38
Not efficient because it produces ATP only by glycolysis. |
|
|
Term
| Summarize what happens in the Kelvin/Citric Acid Cycle (KCAC) |
|
Definition
| P.A. --> CO2, some ADP --> ATP. H atoms are provided for next step. Coenzyme NADH created via oxidization. Thiamine/riboflavin/niacin/pathothenic acid all essential |
|
|
Term
| What happens in the electron transport chain? |
|
Definition
| ATP generated using oxygen (Aeroic respiration) |
|
|
Term
| Summarize the electron transport chain process |
|
Definition
| NADH transfer H to EXC ( e-/p+ to carrier proteins), E- transferred NADH->O2, E- carried along EXC by carrier proteins, p+ shuffled out of cell (proton gradient created), aerobic respiration (carrier protein transferes 2 protons to oxygen to form water), ATP synthase allows outside protons in (proton motive force creates some ATP) |
|
|
Term
| What do hydrolic enzymes do? |
|
Definition
| Break bonds by adding water |
|
|
Term
| What do cellulases, amalayses and B-galactosidase digest? |
|
Definition
| Cellulose, starches, lactose (glucose+ galactose) |
|
|
Term
| What are fatty acids digested by? |
|
Definition
|
|
Term
| What breaks peptide bonds between amino acids? How are amino groups removed? |
|
Definition
|
|
Term
|
Definition
| Complete set of genetic information for a cell |
|
|
Term
|
Definition
| functional unit of genome |
|
|
Term
|
Definition
|
|
Term
|
Definition
| study of function and transfer of genes |
|
|
Term
|
Definition
| study and analysis of nucleotide sequencing of DNA |
|
|
Term
|
Definition
| DNA duplication before cell division |
|
|
Term
|
Definition
|
|
Term
|
Definition
| interprets information carried by RNA to synthesize encoded protein |
|
|
Term
|
Definition
| copies info in DNA to RNA |
|
|
Term
| What is the central theory of molecular biology |
|
Definition
| flow of information from DNA->RNA->Proteins |
|
|
Term
|
Definition
| RNA genome that copies information in form of DNA |
|
|
Term
| DNA has a _' and _' end, the strands are ______. Seperating the strands is ____. |
|
Definition
| 3, 5, antiparallel, denaturing |
|
|
Term
| With regards to RNA, what is a "transcript"? |
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Definition
| A fragment of RNA synthesized from DNA |
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Term
| What is the function of mRNA |
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Definition
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Term
| DNA replication is _____, as each of the 2 newly created DNA strands is half original and half new DNA |
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Definition
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Term
| What is the function of DNA Gyrase? |
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Definition
| temperature breaks DNA down |
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Term
| What is the function of DNA Ligase? |
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Definition
| joins DNA, forms covalent bond between sugar and P of adjacent nucleotide |
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Term
| What is the function of DNA Polymerase? |
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Definition
| synthesizes DNA helix ahead of replication fork |
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Term
| What are Okazaki Fragments? |
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Definition
| nucleic acid fragments that are generated during the discontinuous replication of amino acids |
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Term
| What is the origin of Replication? |
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Definition
| region of DNA where replication begins |
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Term
| What is the function of Primase? |
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Definition
| synthesizes small RNA fragments to serve as primers for DNA synthesie |
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Term
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Definition
| nucleic acid fragment to which DNA polymerase can add nucleotides |
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Term
| What is the Negative (-) strand |
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Definition
| DNA template strand, for RNA synthesis, RNA is complementary |
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Term
| What is the Positive (+) strand |
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Definition
| strand complementary to negative, RNA analogous |
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Term
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Definition
| nucleotide sequence to which RNA polymerase binds to begin transcription |
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Term
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Definition
| component of RNA polymerase that recognizes promoter region |
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Term
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Definition
| sequence of nucleotides to which ribosome bonds |
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Term
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Definition
| Transcript carrying only one gene (poly=multiple) |
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Term
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Definition
| RNA polymerase without a sigma factor |
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Term
| What happens in termination? |
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Definition
| Terminator encountered, ribosome falls off |
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Term
| What happens during transcription initiation? |
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Definition
| At start codon, initiation complex forms |
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Term
| What happens during transcrption elongation? |
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Definition
| Ribosome has 2 sites (P/A). Initiating tRNA binds to P, tRNA recognizing next codon binds to A, enzyme binds with peptide bond, translocates one codon, tRNA released through E (exit) site |
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Term
| What happens during transcrption termination? |
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Definition
| Stop codon encountered, enzymes called release factors free tRNA |
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Term
| What are the function of chaperones? |
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Definition
| Help fold protein into final shape |
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Term
| How are proteins meant to travel outside the cell differentiated? |
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Definition
| Signal sequences added at the ends (tagged for export) |
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Term
| What are spontaneous mutations |
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Definition
| Mutations that occur in a cells natural enviornment in an exteremely (1 in 10k to 1 in 1T) |
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Term
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Definition
| The random reverseal of mutation (to a non mutated form) |
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Term
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Definition
| Incorrect base incorporation into DNA |
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Term
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Definition
| A base changes but due to degenerate gene code, new codon specifies same amino acid |
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Term
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Definition
| New codon specifies new amino acid. Leaky - cell grows slowly, as encoded protein still partially works |
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Term
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Definition
| New codon is stop, protein truncated |
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Term
| Define null/knockout mutation |
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Definition
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Term
| What is used to increase the frequency of base mutations? |
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Definition
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Term
| Why is adding 1 or 2 bases worse than 3? |
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Definition
| Frame shift mutation if 1 or 2 added/removed -- causes ALL following codons to be wrong |
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Term
| Chemicals that affect _________ _______ in DNA will result in higher mutations |
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Definition
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Term
| Define intercalcating agents |
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Definition
| Mutagens that fit between N bases in DNA and distort the structure |
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Term
| How do UV rays affect DNA? |
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Definition
| Cause covalent bonds to form between adjacent thiamine bases (thiamine dimers) |
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Term
| How do X rays affect DNA? |
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Definition
| Cause single and double strands to break (lethal deletions) |
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Term
| What keeps the mutation rate in DNA low, aside from a low frequency of occurance? |
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Definition
| DNA repair (pre or post replication), if heavy damage SOS repair |
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Term
| Define DNA-Mediated Transformation |
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Definition
| Genetic exchange allowing DNA to move bacterium |
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Term
| How does DNA enter bacteria cells? |
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Definition
| Binds to receptor cells on "competent" (state requiring high bacteria concentration, etc.) cells, one strand enters, other is degraded by nucleases |
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Term
| How does donor DNA integrate into the host genome? |
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Definition
| Homologous recombination - If donor DNA has a homologous region in cell genome, it will take its place |
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Term
| What does transduction allow? |
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Definition
| Bacterial DNA to transfer to another bacteria, mediated by bacteriophage |
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Term
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Definition
| DNA/RNA with protein coat, infect by injecting nucleic acid |
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Term
| Describe the process of transduction |
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Definition
| Injection, nucleic acid removes host DNA, phage coat proteins synthesize, phage DNA replicates, some phage heads envelope bacterial DNA and infects another cell, bacterial DNA integrates into chromosomes by homologous recombination |
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Term
| What does conjugation allow? |
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Definition
| Bacterial DNA transfer, requires physical contact (bacterial sex) |
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Term
| What does conjugation involve? |
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Definition
| Mobilization of DNA transfer, plasmid transfer, synthesis of functional plasmid inside recipient/donor cells |
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Term
| Light of a _______ wavelength will provide better resolution because these \waves can more easily fit between individual objects in the specimen. |
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Definition
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Term
| True of False: Differential media suppresses the growth of unwanted bacteria but promotes the growth of wanted bacteria |
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Definition
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