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micro
CH99
83
Microbiology
Undergraduate 1
06/04/2013

Additional Microbiology Flashcards

 


 

Cards

Term
Standard methods of sterilization are NOT effective in inactivating_______.
Definition
prions.
Term
Standard methods of sterilization ARE effective in inactivating which 5 things?
Definition
viruses, bacterial cells, bacterial endospores & fungi.
Term
Disinfectants are used on ______ surfaces.
Definition
inanimate
Term
microbial death is an effective means of evaluating the efficacy of ______ agents
Definition
antimicrobial
Term
microbial death can be used to evaluate the efficacy of _____?
Definition
antimicrobial agents.
Term
microbial death is ______ over time of exposure to an antimicrobial agent.
Definition
constant
Term
microbial death is the permanent loss of a microbe's ability to _____ and can be used to evaluate _______
agents.
Definition
reproduce, antimicrobial
Term
Give an example of sanitization (from the book)
Definition
A public toilet is treated with disinfectants.
Term
Aseptic means free of ______?
Definition
ALL pathogens.
Term
What are are some examples of things that are bacteriostatic?
Definition
desiccation, freezing below 0°C, lyophilization & refrigeration of mesophiles.
Term
Antimicrobial agents that damage nucleic acids also affect ______?
Definition
protein synthesis
Term
Seventy percent alcohol is effective against_____?
Definition
enveloped viruses
Term
An instrument that will come into contact with only the skin of a patient should be disinfected with a _____?
Definition
low-level germicide.
Term
Is lyophilization an effective means of sterilization?
Definition
No
Term
What of ARE effective means of sterilization? Name 4
Definition
ionizing radiation, incineration, autoclaving & dry heat
Term
heating at 72°C for 15 seconds describes what type of pasteurization?
Definition
flash pasteurization
Term
What is the dairy creamer used in restaurants is usually sterilized by?
Definition
UHT sterilization.
Term
Boiling water for 10 minutes is effective in ridding it of______?
Definition
both growing bacteria and enveloped viruses.
Term
What are some features associated with filtration?
Definition
1.nitrocellulose or plastic membrane filters
2.sterilization of heat-sensitive materials
3.use of HEPA filters to filter air
4.ability of some filters to trap viruses and proteins
Term
What is a target of pasteurization?
Definition
Brucella melitensis
Term
ultraviolet light radiation is _____ and has the _____ wavelength
Definition
nonionizing, shortest
Term
gamma rays are used for microbial control in?
Definition
fresh fruits and vegetables
Term
HEPA filters and ultraviolet light can be used to disinfect _____?
Definition
air
Term
What are some of the groups of chemical antimicrobial agents?
Definition
1.halogens
2.phenolics
3.alcohols
4.aldehydes
5.surfactants
6.heavy metals
Term
Lysol is an example of which group of chemical antimicrobial agents?
Definition
phenolics
Term
Which of the following is the most appropriate pairing of microbe and biosafety level?
A) E. coli, BSL-3
B) anthrax, BSL-1
C) Ebola, BSL-2
D) methicillin-resistant Staphylococcus aureus (MRSA), BSL-2
E) tuberculosis, BSL-1
Definition
methicillin-resistant Staphylococcus aureus (MRSA), BSL-2
Term
Betadine is an example of which group of antimicrobial agents?
Definition
halogens
Term
quaternary ammonium compound are a type of____?
Definition
detergent.
Term
Zephiran is an example of a
Definition
quat
Term
quaternary ammonium compounds are not effective against ______?
Definition
Pseudomonas aeruginosa.
Term
quaternary ammonium compounds are harmless to humans except at _______.
Definition
high concentrations.
Term
peracetic acid is a ______ ?
Definition
a sterilizing agent
Term
silver nitrate was used in the past to prevent the transmission of _____ from an infected mother to her newborn.
Definition
gonorrhea
Term
ethylene oxide is used to sterilize items that should not, or cannot, be exposed to _______ ?
Definition
heat or water
Term
aldehydes denature _____?
Definition
proteins.
Term
Some aldehydes can ______ after long periods of exposure.
Definition
sterilize
Term
aldehydes are usually hazardous to ____?
Definition
humans.
Term
aldehydes are used in _____ solutions?
Definition
aqueous
Term
antimicrobials are
Definition
Disinfecting agents naturally produced by microorganisms
Term
A chemical agent that kills pathogenic microbes in general is a
Definition
germicide.
Term
What is NOT a desirable characteristic of an ideal antimicrobial agent?
Definition
It only arrests growth of vegetative cells.
Term
an ideal antimicrobial agent is ____ during storage.
Definition
stable
Term
an ideal antimicrobial agent is ______? 3 thing
Definition
inexpensive
harmless to humans.
acts quickly.
Term
The endospores of which microbes are used to measure the effectiveness of autoclave sterilization?
Definition
Bacillus stearothermophilus
Term
A scientist develops a new medication that is a protein compound and that must be administered by injection. What would be the most effective and safest means of preparing a sterile solution of the new medication?
Definition
filtration
Term
What procedure is currently the standard test used in the United States for evaluating the efficiency of antiseptics and disinfectants?
Definition
use-dilution test
Term
_______ would be used to sterilize a mattress
Definition
ethylene oxide
Term
Disinfectants that damage membranes include which 2 things?
Definition
alcohol and phenolics.
Term
ethylene oxide is the most ___ to humans.
Definition
toxic
Term
Hydrogen peroxide is a ____ agent.
Definition
disinfectant and sterilizing agent
Term
Filtration is a ____ agent.
Definition
Sterilizing agent
Term
Incineration is a ____ agent.
Definition
Sterilizing agent
Term
Alcohol is a ____ agent.
Definition
Disinfectant
Term
Quats is a ____ agent.
Definition
Disinfectant
Term
Ethylene oxide is a ____ agent.
Definition
Sterilizing agent
Term
Glutaraldehyde is a ____ agent.
Definition
disinfectant and sterilizing agent
Term
Chlorine dioxide is a ____ agent.
Definition
Disinfectant
Term
Gamma irradiation is a ____ agent.
Definition
Sterilizing agent
Term
Boiling water is a ____ agent.
Definition
Disinfectant
Term
Can an environment contain some microbes and still be considered sterile?
Definition
yes
Term
Some viruses are inactivated by the same chemical or physical agents that damage _______ ___________.
Definition
cytoplasmic membranes
Term
Does UV light have the most effect on protein structure?
Definition
No
Term
Antimicrobial agents usually work best at ______temperatures and pH levels.
Definition
low
Term
Is the phenol coefficient one of the most widely used measurements of an antimicrobial agent's effectiveness?
Definition
No
Term
No chemical or antimicrobial agents inactivate prions-True or False?
Definition
False
Term
Is the decimal reduction time the time required to kill all the microbes in a given sample?
Definition
No
Term
_____ freezing is more damaging to microbial cells than _____ freezing.
Definition
Slow, quick
Term
Is Hydrogen peroxide an effective antiseptic?
Definition
No it is not
Term
By themselves, soaps have only ____ activity, not ______ activity.
Definition
degerming, antimicrobial
Term
________ is the process of freeze-drying microbes to preserve them.
Definition
Lyophilization
Term
Natural antiseptics such as pine or clove oil are examples of antimicrobial compounds called ________.
Definition
phenolics
Term
A(n) ________ is an iodine-containing organic compound found in such antiseptics as Betadine.
Definition
iodophor
Term
Disinfectants known as ________ have the chemical group CHO, which reacts with and damages both proteins and nucleic acids.
Definition
aldehydes
Term
The lowest temperature that kills all cells in a broth in 10 minutes is known as the ________.
Definition
thermal death point
Term
The amount of time needed to sterilize materials using moist heat is ________ (greater than/less than) the time needed to sterilize using dry heat.
Definition
less than
Term
A(n) ________ is an instrument that sterilizes by exposing materials to steam under pressure.
Definition
autoclave
Term
The containment level ________ is appropriate when handling highly contagious deadly microbes
Definition
BSL-4
Term
Ultraviolet light penetrates materials ________ (more effectively/less effectively) than gamma rays.
Definition
less effectively
Term
________ is a phenolic antimicrobial compound that has been incorporated into consumer items such as garbage bags and diapers.
Definition
Triclosan
Term
Elements such as iodine, chlorine, and bromine are examples of ________, which are the basis for many effective antimicrobial agents.
Definition
halogens
Term
The use of high levels of salt or sugar in the preservation of foods relies on the phenomenon of ________.
Definition
osmotic pressure
Term
The process of heating milk or fruit juice to levels that kill any pathogenic microbes present is known as________.
Definition
pasteurization
Term
Sterilization procedures generally focus on inactivating or eliminating bacterial ________.
Definition
endospores
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