Term
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Definition
Inoculation
Incubation
Isolation
Inspection
Identification |
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Term
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Definition
| Small amount is introduced to a growth medium. Ex. throat swab |
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Term
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Definition
| Temperature controlled environment to allow multiplication and growth. |
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Term
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Definition
| Separating a single bacteria cells and providing growth media. |
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Term
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Definition
| Water-based that do not solidify at above freezing temps. (Broths, milks, or infusions) |
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Term
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Definition
| Clot-like consistency. Used to determine motility. |
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Term
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Definition
| Firm surface @ room temp. |
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Term
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Definition
| Liquid at higher temps. (Agar) |
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Term
| Non-liquefiable solid media |
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Definition
| Always solid. Rice grains, cooked meat, potato slices. |
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Term
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Definition
| Chemically defined. Pure organic & inorganic substances. |
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Term
| Complex (non-synthetic) Media |
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Definition
| Not chemically defined. Include items which are not specifically known, usually animal or plant extracts (blood agar). |
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Term
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Definition
| Grow a broad spectrum. Contains wide variety of nutrients. (Ex: TSA) |
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Term
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Definition
| Grow fastidious microbes "picky eaters". Contains complex organic substances such as blood & growth factors. (Blood Agar) |
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Term
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Definition
| Allow growth of certain groups of microbes & inhibit other. (EX: PEA) |
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Term
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Definition
| Display differences between organisms. Usually by size, shape, or color. (EX: MS, MacConkey, EMB) |
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Term
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Definition
| Organism is transferred to a slide, usually stained, then viewed. Note cell size, shape, arrangement, and internal & external structure. |
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Term
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Definition
| Studying aspects of a known or unknown organism. Bergey's manual is used. |
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Term
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Definition
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Term
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Definition
| Forms the real image. Light passes around or through. |
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Term
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Definition
| Forms virtual image. Further magnifies into the one we look into. |
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Term
| Total power of magnification |
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Definition
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Term
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Definition
| Ability to distinguish two adjacent objects or points from one another. Increased mag, decreased resolution. |
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Term
| Visible Light microscopes |
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Definition
| Use visible light. Bright-field, Dark-field, phase-contrast, and fluorescence. |
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Term
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Definition
| Light is transmitted through the specimen. Specimen is darker than the surrounding field. Live, unstained specimens & preserved, stained specimens. |
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Term
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Definition
| Adding a stop disc to the condensor. Light goes around specimen. Effective for living cells. Does not allow vision of fine internal details. |
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Term
| Phase-contrast Microscope |
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Definition
| Transforms changes in light waves passing through a specimen into difference in light intensity. In live, unstained cells you can see intracellular structures (spores, granules). |
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Term
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Definition
| UV radiation source and a filter. Used w/ dyes. Used in diagnosing infections caused by specific pathogens using fluorescent antibodies. |
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Term
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Definition
| Forms image w/ beams of electrons. Magnification is extremely high. View finest structure of cells & viruses. |
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Term
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Definition
| Universal diagnostic stain. Developed by Hans Christian Gram in 1884. Purple (+) Red/Pink (-) |
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Term
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Definition
| Diagnostic stain. Pink (+ acid fast) Blue (- not acid fast) |
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Term
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Definition
| Dye is forced by heat into resistant bodies called spores. |
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Term
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Definition
| Used to emphasize certain cell parts that aren't revealed by conventional staining. (Capsule staining & flagellar stain) |
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