Term
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Definition
| -Basically a plasmid that can also be integrated into the chromosome |
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Term
Define;
Mobilizable plasmid
Conjugative plasmid
Self-transmissible plasmid
Plasmid mobilization |
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Definition
-A plasmid that can prepare its DNA for transfer but cannot actually cause its transfer
-A plasmid that can mediate it's own transfer, but not prepare itself
-A plasmid that can both prepare and transfer itself
-One plasmid facilitating the transfer of another in the same cell |
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Term
| What are bacteriocinogenic plasmids? Give some examples and what they do? |
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Definition
-Encode bacteriocins; bactericidal for other bac
Bacteriocins
-Colicin E1; Impairs ATP formation
-Colicin E2; A DNAse
-Colicin E3; Inactives 30S |
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Term
| What are the plasmid-encoded virulence traits of E. coli (4)? |
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Definition
-Colicins; facilitate colonization (kill other bac)
-Capsular K antigen; adherence to intestinal mucosa
-Enterotoxins; diarrheal syndrome
-Epithelial penetration factor; allows intestinal invasion |
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Term
| What are the plasmid-encoded virulence traits for S. aureus, streptococcus? |
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Definition
S. aureus;
-Exfoliatin toxi; causes scalded skin syndrom in infants
Streptococcus;
-Hemolysins; lyse RBCs |
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Term
| What are transposons? Where can they do their thing? |
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Definition
-Transpositional genes (can move around)
-They are able to hop from plasmid to chrom, vice versa, and and from one place to the other on the same molecule
-Can even jump cells with cell-cell contact |
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Term
| What are the conjugation cell types? |
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Definition
-F factor (fertility factor) allows genes to be transferred from one bacterium carrying the factor to another bacterium lacking the factor by conjugation (required to form pili);
F- cell; No F factor (recipient)
F+ cell; Free (plasmid) F factor (donor/male)
Hfr cell; Integrated F factor (in chrom)
-stands for high frequency of recombination
F' cell; Free F factor that has a bit of the chrom with it (from incorrect excision); diploid for a gene |
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Term
| What occurs when a Hfr cell is the donor? |
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Definition
-It attempts to transfer it's entire genome because of the inherent nature of F factor, but the bridge usually breaks and the "female" cell remains F-
-If, on the other hand, an F+ cell were the donor, the recipient cell would end up F+ |
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Term
| What if an F' and F- cell mix? |
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Definition
| -The F- will end up F' (duh) |
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