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Bacteriology Mycology Protozoology Parasitology Phycology |
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Infection control Epidemiology |
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Industrial microbiology Pasteurization - food and beverage tech |
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Microbial metabolism Genetics Genetic engineering |
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| Koch's postulates -> Etiology |
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Environmental microbiology Ecological microbiology |
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Antiseptic medical techniques Hospital microbiology |
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| Jenner von Behring & Kitasato |
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| Pharmaceutical microbiology |
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1. Suspected pathogenic organism should be present in all cases of the disease and absent from healthy animals 2. Suspected organism should be grown in pure culture 3. Cells from a pure culture of the suspected organism should cause disease in a healthy animal 4. the organism should be reisolated and show to be the same as the original |
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| Classification of bacteria |
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Definition
Morphology Staining characteristics rRNA sequences Physiological properties Environmental considerations diseases that they cause |
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isolate dna PCR squence analysis generate phylogenetic tree |
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| pathogens cause infectious diseases |
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| regulating an enzyme by binding an effector molecule at allosteric site (not active site) |
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| a reversible metabolic reaction; a reaction that can be catabolic or anabolic |
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| all of the synthesis reactions in a molecule |
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| type of cellular respiration not requiring ocygen atoms as final electron acceptors |
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| antimicrobial agent produced naturally by an organism |
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| one antibiotic with least effect interferes with effects of another |
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| combinations of drugs that improve efficacy of antimicrobials |
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| any compound used to treat infectious disease; may also function as intermediate level disinfectant |
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| chemicals used to inhibit/kill microorganisms on skin or tissue |
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| woese's taxonomy; domain which includes all prokaryotic cells having archael rRNA sequences |
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| characteristic of an environment or procedure that is free of contamination by pathogens |
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| microorganisms requiring acidic pH |
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| actin structures that network off the cell to facilitate movement |
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| the amount of energy needed to trigger a chemical reaction |
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| functional site of an enzyme, the shape of which is complementary to the shape of the substrate |
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| the movement of a substrate against its electrochemical gradient via carrier proteins; requires cell energy in the form of ATP |
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| attachment of microorganism to host cells |
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| an organism that uses oxygen as a final electron acceptor |
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| type of cellular respiration requiring oxygen atoms as final electron acceptors |
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| movement towards oxygen/air |
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| can live in an environment with oxygen present |
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| gel-like polysaccharide isolated from red algae and used as a thickening agent |
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| measures the effect of an antimicrobial agent |
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| eurkaryotic multi or unicellular photosynthetic organisms with simple reproductive structures |
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| organism requiring high pH environments (alkaline) |
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| the number of species living in a given ecosystem |
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| slimy community of microbes growing on a surface |
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| living things only come from other living things |
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| time it takes blood plasma concentration of something to half |
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| the use of microorganisms to remediate toxins in the environment to reclaim soils and waterways |
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| the region of earth inhabited by living organism |
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| branch of microbiology in which microbes are manipulated to manufacture useful products |
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| antimicrobial that works against many different kinds of pathogens |
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| in prokaryotes and yeasts, reproductive processes in which an outgrowth of the parent cell recieves a copy of the genetic material, enlarges and detaches |
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| glycocalyx composed of repeating units of organism chemicals firmly attached to the cell surface |
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| all the decomposition reactions in an organism |
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| catalyzes decomposition of hydrogen peroxide |
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| in most cells, structural boundary composed of polysaccharide or protein chains that provide shape and support against osmotic pressure |
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| the primary, short term, recyclable energy molecule fueling cellular reactions |
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| device that used steam heat under pressure to sterilize chemicals and objects that can tolerate moist heat |
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| makes its own molecules of food |
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| rod shaped prokaryotic cell |
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| prokaryotic microorganism typically having cell walls composed of peptidoglycan. all cells with bacteria rRNA sequences |
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| protein toxins produced by bacteria to inhibit growth of similar strains |
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| causing bacteria to lyse/die |
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| large scale closed system culture |
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| bacterial enzyme that breaks the beta-lactam ring of penecillin and similar molecules rendering them inactive |
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| the most common method of asexual reproduction of prokaryotes, in which the parental cell disappears with formation of progeny |
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| the classification method used in the linnaen way of taxonomy, which assigns each species both a genus name and a specific ephithet |
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| central metabolic pathways |
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Definition
| the pathways used by bacteria to process major carbohydrates/other carbon sources for energy |
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| uses chemicals/organic molecules for energy as its carbon source (storage of energy) |
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| directed movement towards certain chemicals |
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| a chemical that has the potential to destroy pathogenic microorganisms |
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| requires organic substrates to get carbon source |
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| light harvesting organelle found in photosynthetic eukaryotes |
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| molecule of DNA associated with protein. in prokaryotes usually circular in area called nucleoid |
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| a prokaryotic cell intermediate in shape between a sphere and a rod, elongated coccus |
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| spherical prokaryotic cell |
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| visible population of microorganism living in one place, aggregation of cells avising from single parent cell |
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| when an inhibitory substance blocks enzyme activity by blocking active sites |
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| the amount of time water is allowed to contain the disinfectant to assure potability (retention time) |
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| membrane surrounding all cells, and composed of a fluid mosaic of phospholipids and proteins |
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| internal network of fibers contributing to the basic shape of eukaryotic and rod shaped prokaryotic cells |
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| phase in a growth curve in which organisms are dying more quickly then they are being replaced |
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| positively charged organic sufactant |
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| seuqential usage of two different sugars by certain bacteria |
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| the use of physical/chemical means to kill/inhibit microorganisms on inanimate objects |
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| deoxyribonucleic acid, made up of base pairs, makes up genetic material |
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| any of the three basic types of cell groupings distinguished by Carl Woese, containing Linnaean taxon of kingdoms |
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| specific behaviors/heirachy and interaction of an organism with it's ecosystem |
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| all the organisms living in a particular habitat |
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| series of redox reactions that pass electrons from one membrane bound carrier to another then to a final electron acceptor |
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| environmentally resistant structure produced by the transformation of a vegetative cell of genera bacillus or clostridium |
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| (lipid A), potentially fatal toxin released from the cell wall of dead/dying gram- bacteria |
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| special flagella of spirochetes taht spirals tightly around a cell, rather than protruding from it |
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| gram- bacteria with pathogenic and nonpathogenic strains; nonpathogenic are symbiotic and live in the gut |
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| very toxic gas used as a disinfectant for heat sensitive materials |
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| study of causation of disease |
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| type of cell that has a nucleus |
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| extracelluar enzyme, usually breaks down large molecules for entry into cell, eg.emylase |
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| microorganism which can live with or without oxygen |
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| to FADH2, carries high energy electrons for oxidative phosphorylation |
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| (negative feedback) method of controlling the action of enzymes in which the end product in a series of reactions inhibits an enzyme in an earlier part of the pathway |
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| in metabolism, the partial oxidation of sugar to release energy using an endogeneous molecule instead of an electron transport chain |
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| what is visible through microscope |
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| sticky, proteinaceous extensions of some bacterial cells that function to adhere cells to one another and to environmental surfaces |
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| eukaryotic organisms that have cell walls and obtain food from other organisms |
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| first step in catabolism of glucose via respiration/fermentation |
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Definition
| integral membrane proteins, help with cell recognition, especially in immune system |
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| pink stained, thin layer of peptidoglycan outer layer |
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Definition
| purple stained thick layer of peptidoglycan, lipotechoic acid |
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| active process by which substance being actively transported across cell membrane is chemically changed during transport |
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| chemicals required in small amounts for metabolism (eg vitamins) |
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| time required for cell to grow and divide |
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| sticky external sheath of prokaryote and eukaryotic cells |
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organisms requiring >9% NaCl salt loving |
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| organisms that prefer extreme heat |
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| deposited substance such as a lipid, gas vesicle stored within cytosol of cell |
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| TCA cycle, 8 enzyme catalyzed cycles that transfer stored energy from acetyl CoA to coenzymes, NAD + FAD |
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| phase in growth curve in which the organisms are adjusting to their environment |
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| LPS component released from dead gram - cells, endotoxin |
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| molecule composed for Lipid A and polysaccharide found in gram - walls |
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| phase in growth curve in which population is most actively growing |
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| having two or more flagella at one end |
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| antibacterial protein secreted in sweat/tears |
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| organism that prefers room temperature |
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| microorganism that requires low levels of O2 |
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| minimal inhibitory concentration (MIC) |
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Definition
| smallest amount of drug that inhibits a pathogen |
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| coenzyme in redox reactins, carries electron from one cycle to another |
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| two glucose derivatives that make up pepridoglycan, OB bond @ C14 -> 5 c rings |
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| biogeochemical cycle involving nitrogen fixation, ammonification, nitrification, denitrification and anammox reactions |
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| oxidative phosphorylation |
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| the use of energy from redox reactions to attach inorganic phosphate to ADP |
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| heating foods/liquids to kill microorganism that cause spoilage |
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| pentose/phosphate pathway |
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| enzymatic formation of phosphorylated pentose sugars from glucose 6-phosphate |
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| in gram -, space between cell membrane and outer membrane with peptiglycan/periplasm |
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| membrane transport proteins, passive transport |
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Definition
| compound derived from phenol molecules that have been chemically modified to denature proteins/disrupt cell membranes |
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| use of energy from light to attach inorganic phosphate to ADP |
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| tubule involved in bacterial conjugation |
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| plasma membrane pulls away from cell wall, lack of water (osmosis0 |
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| in cell morphology used to describe a variabily shaped prokaryotic cell |
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directly involved in growth/development compound synthesized during the log phase of growth |
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| digests amino acids outside cell wall |
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| storage of energy across a membrane, uses electron transport chain to create gradients |
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| cell wall completely removed, gram pos cell lost peptidoglycan layer |
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| single celled eukaryotes that lack a cell wall and are similar to animals |
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survives in cold temps -5 to 20 |
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| decomposition of proteins (stage of decomposition) |
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| the ability to distinguish between objects that are close together |
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| antimicrobial that works against fe types of pathogens |
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| ribosomal, form of RNA which makes up ribosomes |
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| process of disinfecting surfaces and utensils used by public |
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| not directly involved in growth/development |
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| loose, water soluble glycocalyx |
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| cell wall partially removed, gram - cell lost peptidoglycan layer |
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| stiff, spiral shaped prokaryotic cell |
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| helical, corkscrew shaped bacteria (gram -) |
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| phase in a growth curve in which new organisms are being produced at same rate as old dying |
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| using dyes to color cells, simple = 1 dye, complex = multiple dyes |
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| eradication of all organisms including endospores and viruses |
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| substrate level phosphorylation |
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| transfer of phosphate to ADP |
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| science of naming and classifying organisms |
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| organism that lives in high temp environments |
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| slightly curved rod shaped cell |
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| in a diffusion susceptibility test, a clear area surrounding drug-soaked disk where microbe doesn't grow |
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| yeast, model euk organism |
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| cause of pneumonia, bacterial meningitis |
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| genus of coccus gram +, grow in bunches |
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| genus of coccus gram +, grow in chains |
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1. pilus extends 2. pilus attaches 3. pilus retracts, pulling cell |
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| what do bacteria need to grow |
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Definition
carbon source energy source required elements C,N,O,P,S,Na,K,Cl,Fe,Mg,Ca trace elements copper, manganese, selenium, zinc |
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light - energy source CO2 - carbon source |
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organic compounds - carbon source Light - energy source |
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energy source- chemical compounds carbon source - carbon dioxide |
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organic compounds - carbon source chemical compounds - energy source |
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found in gram negative bacteria, exchange of substances between cytoplasm and periplasm by highly specific transport systems change between periplasm and environment occurs via porins |
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random - no structure needed Swimming - flagella Twitching - type IV pili Pushing - host-cel actin Gliding - secreting polysaccharide |
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| means of growth measurement |
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direct cell counts viable cell counts measuring biomass measuring cell products |
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temp oxygen requirement pH water availability solute concentration |
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| helps cells deal with oxygen - use oxygen as a terminal electron acceptor in ETC |
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| Presence of oxygen - harm bacteria how? |
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Definition
oxygen is highly reactive (oxidizing agent) capable of degrading organic molecules (oxidizing) |
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| osmotic pressure affect on growth |
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affects shape of cell - shrink if hypertonic expand if hypotonic |
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| Factors affecting enzyme function |
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presence/absense of cofactors and catlytic site temp/pH -> denature |
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| introduction of group of microbes to treat contaminated soil or water |
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| protein made by one bacterium which can kill a closely related bacterium |
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