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Micro quiz methods of sterilization
methods section of quiz
18
Microbiology
Undergraduate 2
03/16/2011

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Term
Methods of Sterilization
Definition
1. Hot air Oven
2.autoclave
3. Filtration
4. Gas Sterilization
5. Strong Chemical Sterilization
/disenfection
Term
Hot Air Oven
Definition
1. Kills by Protein Oxidation
2. 160-70 C for 1-2 hours
3. For glassware,pippetts,oils,powders
Term
Autoclave
Definition
1. Kills by protein Denaturation
2. Live steam under pressure "heat of condensation to microbial cells.
3. 121 at 15psi for 30 minutes.
4. for instruments,simple media,meatalware.
Term
Filtration
Definition
Hepa Filter.
1. Can Remove all bacteria greater than .3 micrometers
2. some filters have .01 mmbrane pores for viruses.
3. Used for any injectable fluids and hemodialysis fluids. (prevents endotoxin effects.)
Term
Gas Sterilization-Ethylene Oxide Gas
Definition
1. Kills by Alkylation
2. 2-12 hours
3. Plastics,electronics,rubber items.
Term
Gas Sterilization-Chlorine Dioxide
Definition
1.Short lived gas used in closed buildings with Anthrax Spores.
2. used to fumigate
Term
Gas Sterilization-Ultraviolet Light
Definition
1. Used to decrease airborne infection.;
2.High Humidity Limits use.
3. Hospital rooms,nursuries,or rooms, cafeterias.
Term
Strong Chem Ster-Ethyl Alcohol (70%)
Definition
Generally a strong degerming
Term
Strong Chem ster/Disenfection-Plasmas
Definition
1. Electromagnetic exitation of gas
2. Used for surgical instruments used in Arthroscopic or Laparoscopic procedures.

Placed in containers with vacuum,electro mag feild, chms like hydrogen proxide which form plasma.
Term
Strong Chem ster/ Disenfection- Aqueous or Gaseous Formaldehyde-
Definition
1. Works at 25C
2. Used Formaldehyde Crystals which were vaporized to kill Ebola Virus.
Term
What Kills the Ebola Virus?
Definition
Gaseous Formaldehyde/Crystals
Term
What Kills the Ebola Virus?
Definition
Gaseous Formaldehyde/Crystals
Term
What Kills the Ebola Virus?
Definition
Gaseous Formaldehyde/Crystals
Term
What Kills the Ebola Virus?
Definition
Gaseous Formaldehyde/Crystals
Term
Strong Chem Sterl/Disen-Gluteraldehyde(SIDEX)
Definition
Strong disenfectant for surgical instruments/dental tools.
Term
Strong Chem- Amphyl
Definition
Floors Walls and furniture. (hospital use primarily)
Term
Strong Chem Sterl/disen- Bleach
Definition
1.Antiviral oxidizing agent
2. rooms and equipment
3. Used by CDC apartments/hotels/homes etc.
Term
Purpose Of Sterilization
Definition
To prevent contamination of materials,media, and patients by bacteria, protozoa,fungi and viruses.
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