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Micro Mid-Term
Mid-Term Practice
127
Microbiology
Undergraduate 1
10/17/2012

Additional Microbiology Flashcards

 


 

Cards

Term
Sterilization
Definition
A process that destroys or removes all microbes including viruses. Such as autoclaving and ionizing radiation
Term
Two types of energy captured by chloropasts
Definition

1) energy from sun captured in plants

2) energy captured by our consumption of food

Term
Algae
Definition
A group of photosynthetic organisms most readily recognized by their larger members such as seaweed and kelp
Term
Nucleic Acid
Definition

Can be double stranded DNA

Single strande DNA

double stranded RNA

single stranded RNA

Term
RNA and DNA in viruses
Definition

RNA is normally only single stranded and DNA is double stranded

However, in viruses both RNA and DNA can be single and double stranded

Term
Genome
Definition
The subtotal of the genetic info carried by an organism
Term
Cellular Respiration
Definition
Begins at our consumption of food
Term
Aerobic Respiration
Definition
requires oxygen and we as humans only utilize Aerobic Respiration...not Anaerobic Respiration
Term
What are the 6 steps of Viral Multiplication
Definition

1) Adsorption - virus attaches to host cell by specific binding of its spikes ot celll receptors

2) Penetration - the virus is engulfed into vesicle and its envelope

3) Uncoated - thereby freeing the viral RNA/DNA into the cell cytolasm

4) Synthesis - Replication and protein production under control of the viral gene the cell synthesizes the basic components of new viruses: RNA molecules, capsomers, and spikes

5) Assembly - viral spikes proteins are inserted into cell membrane for the viral envelope nucleocapsid is formed from RNA and capsomers

6) Release - Enveloped virus is released and the new virion is ready to infect another cell

Term
Reproductive Strategies
Definition

Asexual - piece breaks off and a new piece grows

 

Sexual - spores that germinate

Term
Helminths
Definition
the biggest microorganism and can be seen with the naked eye
Term
Viruses
Definition

inert, just chilling, they are nothing and have nothing

 

Just a capsid and nucleic acid

Term
The capsid of any virus is made up of
Definition
a number of identical protein subunits known as capsomers
Term
Vector
Definition
a source of bacteria as in the mosquito carrying molaria
Term
Enzyme Structure
Definition
Primary structure of an enzyme is protein with some exceptions
Term
Simple Enzyme
Definition
primary structure is protein
Term
Conjugated Enzyme
Definition
protein and non-protein molecules
Term
Study of microbiology
Definition
Study bacteria viruses fungi protozoa and algae which are all collectively called microorganisms or microbes
Term
Dimorphic
Definition
Fungal cells that can take the form of yeast or hyphae depending on growth conditions
Term
Bacteriophages
Definition
Viruses that attack bacteria
Term
Lytic Cycle
Definition
When virus goes into host cell, reproduces and eventually kills the host cell
Term
Lysogenic State
Definition
when virus attacks the host cell and stays dormant in cell; host doesn't die in this stage
Term
Pseudohypha
Definition

Some yeast species form a pseudohypha, a chain of yeast formed when buds remain attached in a rowm.  Because of its manner of formation, it is not a true hypha like that of molds

 

It appears to have septum but doesn't; it's just the unseparated bud

Term
2 Types of microscopic fungi
Definition
Hyphae & Yeasts
Term
Yeast fungi
Definition
distinguished by its round to oval shape and by its mode of asexual reproduction
Term
Hyphae Fungi
Definition
long threadlike cells that make up the bodies of filamentous fungi
Term
Viruses - intracellular parasite
Definition
because viruses lack ribosomes they must go into a cell with ribosomes making viruses an intracellular parasite attaching to ribosomes for protein and nucleus to transfer nucleic acid
Term
Mycellium
Definition
a reproductive force of fungi as in sexual - spores
Term
Fungi
Definition
normally black furry and/or white creamy
Term
DNA Virus
Definition
there are only
6 DNA viruses
Double strands:
Envelope:
Smallpox, Herpes, Hepandna
Nucleocapsid: Adenovirus and Papillomavirus
Single Strands
Nucleocapsids: Parvovirus
Term
Enzymes
Definition
a special class of proteins essential to the chemical reactions of life.  They are catalysts, i.e. speed up chemical reactions
Term
Constuitive Enzymes
Definition
always present and in relatively constant amounts regardless of substrates
Term
Regulated Enzymes
Definition
The production of which is either turned on (induced) or off (repressed) in response to changes in the amount of substrate
Term
Protozoan - Form and Function
Definition
Most are single cells, containing the major eukaryotic organelles except chloroplasts, most have a life cycle
Term
Thylakoids
Definition
Inside the chlorophyll of plants that reacts to the suns energy and sets off the reaction of photosynthesis
Term
Pathways involved in Aerobic Respiration
Definition

1) Glycolysis - breaking down of glucose

2) Krebs cycle - produce ATP and COin the mitochondria

3) Electron transport Chain - transport Hydrogen to Oxygen to create H2O

 

The final electron receptor is O2

Products of Aerobic Respiration are:

ATP, H2O, CO2

Term
Virus holds it's cell hostage
Definition
Everytime the cell duplicates it's own DNA it is duplicating the RNA/DNA of the virus

It can also be reverse transcriptive going from RNA to DNA
Term
Two types of Fungi Infections:
Definition
Superficial - skin i.e. ringworm

Systemic- inside the body most will grow both as a hyphae and yeast enabling a higher chance of survival
Term
Dimorphic
Definition
Fungi infections that can grow as both hyphae and yeast for the utmost chance of surival
Term
Yeast Cell growth
Definition
yeast cells grow buds on its surface which break off and become separate cells i.e. asexual
Term
Mitosis
Definition
One cell splits into two identical cells: as in skin cells and the rest of the human body
Term
Meiosis
Definition
Combination of 2 cells (father & mother cell) combine to form 4 cells for a zygote/embryo
Term
Conjugated Enzyme aka holoenzymes
Definition
a combination of a protein, now called the apoenzyme, and one or more cofactors. Cofactors are either organic molecules or inorganic elements (metal ions)
Term
Fermentation
Definition
when the body isn't getting enough oxygen, it starts the fermentation process and produces lactic acid
Term
Ribosomes
Definition
protein synthesizers
Term
Hydrolysis
Definition
to break down
Term
Glycolysis
Definition
is to break down glucose & occurs in the cytoplasm
Term
Hans Christian Gram
Definition
developed the 130yr old method of gram staining that creates ready differentiation of bacteria

gram positive = purple
gram negative = red
The difference in positive and negative is due to cell wall variation
Term
Loose Glycolax
Definition
produces a slime layer
Term
Strong Glycolax
Definition
a strong capsule composed of repeating polysaccharide units blocking phagocytes...leaving the bacteria free to multiply and infect body tissue more readily
Term
Mycoplasma
Definition
a bacteria that cannot be stained because it doesn't have a cell wall
Term
Parasites and pathogens
Definition
Microorganisms that invade the bodies of hosts often causing damage thru infection and disease
Term
Ubiquitous
Definition
Everywhere
Term
Photosynthesis
Definition
A process where algae and certain bacteria trap the sun's energy to produce food
Term
Viruses
Definition
They are microbes but not cells. They infect prokaryotic or eukaryotic hosts in order to reproduce themselves
Term
Biotechnology
Definition
Applies the power of microbes for the manufacture of industrial products food and drugs
Term
Recombinant DNA
Definition
To intentionally alter genetic material to produce new products and modified life forms
Term
Bioremediation
Definition
Microbes are used to clean up pollutants and waste in natural environments
Term
Pathogens
Definition
Nearly 2000 microbes are pathogens that cause infectious disease
Term
Leeuwenhoek
Definition
Discovered organisms that he called animalcules.
Term
Immunology
Definition
Studies the complex web of protective substances and reactions cause by invading microbes and other harmful entities and include such diverse areas of blood testing vaccination an allergy
Term
Order of taxonomy
Definition
Going from least specific to specific

domain Kingdom phylum class order family genus and species
Term
Procaryotic cell types are placed in the domains archaea and bacteria
Definition
Eucaryotes are all placed in the domain Eukarya
Term
Taxonomy
Definition
The hierarchial scheme for the classification identification and nomenclature of organisms which are grouped in categories call taxa
Term
Abiogenesis
Definition
The notion of spontaneous generation
Term
Biogenesis
Definition
New organisms arise out of existing organisms
Term
Phylogeny
Definition
Records displayed in the form of a tree of life that shows organisms relatedness
Term
Atomic number
Definition
Number of protons in an element
Term
Mass number
Definition
Equal to the number of protons and neutrons
Term
Orbitals
Definition
Pathways for electrons
Term
Organic molecules
Definition
Contain both carbon and hydrogen. All other substances are inorganic chemicals because they lack these on elements
Term
Carbon
Definition
Is the backbone of biological compounds because of its ability to form single double or triple covalent bonds within itself and many different elements
Term
Carbohydrates
Definition
Are biological molecules who's monomers are simple sugars or monosaccharide linked together by glycosidic bond to form polysaccharides
Term
Covalent bond
Definition
2 molecules share electrons

The molecules share the electrons and the balance of charge will be polar if unequal or nonpolar if equally shared electrically neutral
Term
Ionic bonds
Definition
Where are electrons are transferred to an atom that can come closer to filling up the outer orbital
Term
Hydrogen bonds
Definition
Involve week attractive forces between hydrogen and nearby oxygen and nitrogen as in the case of a mosquito standing on water
Term
Solution
Definition
A combination of a solid liquid or gaseous chemical (the solute) dissolved in a liquid medium (the solvent) and water is the most common solvent in natural systems
Term
Inorganic compounds
Definition
Do not contain both carbon hydrogen in combination
Term
Monosaccharides
Definition
Such as glucose are the simplest carbohydrates with 3 to 7 carbons
Term
Disaccharides
Definition
Such as lactose consist of 2 monosaccharides joined by glycosidic bonds
Term
Polysaccharides
Definition
Such a starch and Peptidoglycans chains of five or more monosaccharides
Term
Lipids
Definition
Contain long hydrocarbon chains and are not soluble in polar solvents such as water due to their nonpolar hydrophobic character. Common components of fats are fatty acids elongate molecules with a carboxylic acid group examples are triglycerides phospholipids sterols and waxes
Term
Amino acids
Definition
The basic building blocks of proteins.
Term
Nucleotides
Definition
The building blocks of nucleic acids
Term
Deoxyribonucleic acid DNA
Definition
polymer of nucleotides that occurs as a double stranded helix with hydrogen bonding in pairs between the helices. DNA is the master code for a cell's life processes and must be transmitted to the offspring through replication
Term
Ribonucleic acid. RNA
Definition
Polymer of nucleotides where the sugar is ribose and the uracil is used instead of thymine. it is almost always found single stranded and is used to express the DNA code into proteins
Term
Adenosine triphosphate. ATP
Definition
Contains a nucleotide and is involved in the transfer and storage of energy in cells
Term
Gram stain
Definition
Step 1 apply crystal violet primary dye
Step 2 grams iodine which is your mordant
Step 3 alcohol which is the decolorizer
Step 4 apply safranin red dye counterstain

This is a differential stain
Term
Types of microbiological stains
Definition
simple stains , differential stains which include a Gram stain the acid-fast stain and a spore stain and then you have structural stains which includes the India in capsule and the flagella stain
Term
The 6 I's in microbiology lab techniques
Definition
Inoculation is the first step obtain culture
Isolation of the microorganism
Incubation so they can grow
Inspection information gathering and identification
Term
Properties of a microscope
Definition
Magnification and degree resolution
Term
Common bacterial shapes
Definition
Coccus
Rod/bacillus
Vibrio
Spirillum
Spirochete
Branching filaments
Term
Cells
Definition
All cells must have the minimum structure of an outer cell membrane cytoplasm a chromosome and ribosomes
Term
Metabolism in terms of cells
Definition
This refers to the chemical reactions in the cell including the synthesis of proteins on ribosomes and capture and release of energy using ATP
Term
Appendages of bacteria
Definition
Flagella for motility
Fimbriae to help adhere
Pili provide a means for genetic transfer
Term
Bactericide
Definition
Chemical that destroys bacteria
Term
Bacteriostatic
Definition
Agents inhibit or prevent the growth of bacteria on tissues or on other objects in the environment
Term
Germicide
Definition
Chemical that will kill any pathogenic microorganism
Term
Disinfection
Definition
Refers to a physical or chemical process that destroys vegetative pathogens but not bacterial endospores
Term
sanitization
Definition
Any chemical technique that removes microorganisms to safe levels or standards
Term
How anti microbial agents work
Definition
Their modes of action affect cell wall synthesis membrane permeability and protein and nucleic acid synthesis and function
Term
Autoclave
Definition
Utilizes steam under pressure to sterilize heat resistant materials WHEREAS intermittent sterlization can be used to sterilize more delicate items
Term
pasteurization
Definition
Subjects liquids to temperatures below 100 degree Celsius and is used to lower the microbial load in liquids. boiling water can be used to destroy vegetative pathogens in home
Term
Dry heat sterilization using higher temperatures than moist heat can also be used to sterilize such as incineration
Definition
Incineration can be carried out using a Bunsen burner or incinerator. temperatures range between 800 degrees Celsius and 6500 degrees
Term
Radiation
Definition
Energy in the form of radiation can be used for cold sterilization which works by killing microbes without heat
Term
Ionizing radiation
Definition
Such is gamma rays and x-rays has deep penetrating power and works by causing breaks in the DNA of target
organisms
Term
Non ionizing radiation
Definition
Uses ultra violet waves with very little penetrating power and works by creating dimers between adjacent pyramidines which interferes with replication
Term
Decontamination
Definition
The destruction removal or reduction in number of undesirable microbes an example of this would be asepsis disinfection sanitization and degermation
Term
Sepsis
Definition
The growth of microorganisms in the tissues such as infected wounds and blood infection
Term
Asepsis
Definition
Techniques that prevent the entry of microorganisms into sterile tissues. Such as cleansing the skin with iodine prior to surgery and using sterile needles
Term
Antiseptic
Definition
Chemicals applied to body surfaces to destroy or inhibit vegetative pathogens. Iodophors, antibacterial soap and chlorhexidine
Term
Disinfection
Definition
Destruction of vegetative pathogens on inanimate objects. 5% bleach or boiling water
Term
Sanitization
Definition
Cleansing technique that removes microorganisms and debris from inanimate services
Term
Degermation
Definition
Cleansing technique that removes microorganisms and debris from living tissue. As in surgical hand scrub and alcohol wipes
Term
Sterilization
Definition
The removal or destruction of all viable microbes. Autoclaving and ionizing radiation
Term
Acid fast stain
Definition
Step 1 prepare and mix smear on slide: put bacteria on, let it dry, and heat fix
step 2: suspend slide over a retention container via glass rod, over a plastic container or sink
step 3: primary staining which is Carbol fusion allow to set for 5 minutes
Step 4: wash off to stain
step 5: decolorize with alcohol then rinse
step 6: apply methylene blue as a counterstain for 60 seconds and rinse
Term
Pasteur
Definition
Proved that disease start from organisms entering the body and he developed pasteurization
Term
Cell membranes are made up of
Definition
Proteins lipids and carbohydrates
Term
Solution
Definition
Is a solvent and solute
Term
Determining pH level
Definition
Ch is determined by counting the hydrogen the more hydrogen the more acidic. PH scale goes from 0 to 14
7 is neutral
Term
Organic chemistry
Definition
Organic chemistry is anything living that contains carbon with a few exceptions. Carbon dioxide is not alive
Term
Carbohydrates are simply carbon hydrated with water
Definition
6 carbohydrates equal glucose
5 carbohydrates equal ribose
Term
Hydrolysis
Definition
Adding water
Term
Properties of a microscope
Definition
Magnification
definition of resolution
Term
Peptidoglycan is what makes up a cell wall
Definition
strong surface coating attached to the cell is considered capsule
loosely attached to the cell is slime layer
Term
Mycoplasm
Definition
The only bacteria that cannot be stained because it doesn't have a cell wall
Term
Aerobic respiration
Definition
Glycolysis in the cytoplasm
the Krebs cycle in the mitochondria produce ATP
And co2
Then the electron transport chain carries the hydrogen to the final receptor which is oxygen to produce water
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