Term
After the hand washing experiment, where was the most bacteria observed on the plate?
Why is this? |
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Definition
Wash and Scrub sections.
The wash and scrub sections show the release of the normal flora being activated after the transient flora has left. |
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Term
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Definition
| Chemical agent used on living things. Meant to kill most bacteria, some will still remain. |
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Term
| What quadrant would you find that might be transient flora? |
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Definition
| In the Control. The control has darkest and the most concentrated. |
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Term
| Is hand washing still a good idea? Why |
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Definition
| Yes, because it removes the transient flora which is potentially disease causing flora. |
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Term
| What precautions beyond hand washing can you take to protect your patients from your normal flora (or protect you from theirs)? |
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Definition
Gloves- Protective barrier
Masks/ Gown |
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Term
| Name several types of normal flora that we can commonly find. |
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Definition
1) Staphylococcus
2) Streptococcus
3) Escherichia Coli
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Definition
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Definition
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Definition
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Term
How is bacteria colony formed in the first place?
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Definition
1) Originates from one mother cell
2) Divides by binary fusion
3) One colony = millions of bacteria cells
4) All membranes of a colony are the same- identicals |
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Term
| What does turbidity mean? |
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Definition
| Turbidity is when a culture is no longer sterile and shows cloudiness. The more turbid the broth, the more growth there is in the tube |
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Term
| What is a properly prepared smear? |
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Definition
1) It withstands washing during the staining procedure without the loss of bacteria from the slide surface
2) Will not cause distortion of the bacterial cells
3) is not too thick ( so that true shape and arrangements can be observed)
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Term
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Definition
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Term
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Definition
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Term
| What inoculating tool do you use with a liquid media? Give an example. |
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Definition
Inoculating Loop.
A broth |
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Term
| What inoculating tool do you use with a solid media? Give an example |
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Definition
Inoculating needle
A slant |
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Term
| What is the principle behind the way a negative stain works? Give an example. |
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Definition
Nigrosine is an acidic stain. It works because the stain cannot penetrate the cells. Nigrosine is acidic. It has a negative charge, and the bacteria has a negative charge
The two negatives repel each other .
The result is that the background will be dark and the cell walls will be colorless. The contrast allows the eye to see. |
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Term
| Why do we use simple staining technique? Give an example. |
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Definition
| We use a simple strain to color a bacterial smear. Example is using Methylene blue. Methylene blue is a basic dye with a positive charge. We use basic stains more often than acidic stains . Negative bacteria + positive stain allows the stain to penetrate the cell and creates a stained image that is easier to see. |
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Term
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Definition
Escherichia coli
If it is not in italics, it has to be underlined. |
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Term
| Give two main reasons for heat fixing a bacterial smear? |
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Definition
a. kills bacteria
b. attach or adhere bacteria to the slide's surface. |
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Term
| Why must a slide air dry before the slide is then heat fixed? |
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Definition
| Because if we were to boil the liquid we would destroy the cell structure. |
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