Term
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Definition
lens quality
higher power=clearer image |
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Term
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Definition
size of the area that the lens views
larger mag=smaller field |
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Term
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Definition
| specimen in view when objective lens is switched |
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Term
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Definition
| position from lens where object is in sharp focus |
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Term
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Definition
| thickness of layer in sharp focus |
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Term
| how do you calculate total magnification? |
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Definition
| magnification by objective lens X magnification by ocular lens |
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Term
| 5 steps of a wet mount slide |
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Definition
1. small drop of water
2. put sample in water and cover
3. place slide on stage
4. with lowest power lens(4x)(scanning power), focus on sample
5. use coarse adjustment and then switch to fine adjustment to focus |
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Term
| what is the only lens power you can use coarse adjusment on? |
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Definition
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Term
| 4 stains used in a gram stain |
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Definition
1. crystal violet
2. grams iodine-mordant
3. ethanol-decolorizer
4. safranin-counterstain |
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Term
| what color is a gram positive cell? |
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Definition
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Term
| what color is a gram negative cell? |
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Definition
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Term
| 3 characteristics of a negative stain |
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Definition
1. no heat fixation
2. inoculated with nigrosin
3. drop is spread across slide |
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Term
| 3 steps of a capsule stain |
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Definition
1. make a negative stain
2. heat fix-destroys capsule
3. stain with crystal violet |
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Term
| 5 steps of acid fast stain |
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Definition
1. heat fix slide
2. place slide in steam bath with bibulous paper covering smear
3. drip paper with carbol fuschin for 5 minutes
4. decolorize
5. counterstain with methylene blue |
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Term
| what bacteria is most common for an endospore stain? |
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Definition
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Term
| 5 steps of an endospore stain |
|
Definition
1. heat fix bacteria
2. place slide in steam batch with bibulous paper covering smear
3. drip paper with malachite green for 7-10 min
4. decolorize
5. counterstain with safranin |
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Term
| what is flagella used for? |
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Definition
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Term
| where are flagella cultures grown? |
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Definition
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Term
| 4 steps of flagella stain |
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Definition
1. add flagella stain to a dry smear
2. incubate at room temp for 15-20 min
3. add tap water to flood off stain
4. counterstain with safranin |
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Term
| what 3 things are NOT done to a flagella stain? |
|
Definition
1. no blotting
2. no heat fixation
air dry only!!! |
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Term
| define facultative anaerobes |
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Definition
| use oxygen for respiration if it is present |
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Term
| define aerotolerant anaerobes |
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Definition
| can tolerate the presence of oxygen |
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Term
|
Definition
| require small amounts of oxygen |
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Term
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Definition
| gas pack with hydrogen and carbon dioxide, palladium sequesters oxygen gas into water |
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Term
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Definition
| oxygen gradient is formed throughout agar as it hardens |
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Term
| define thyoglycolate media |
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Definition
| oxygen diffuses in from top of tube, producing an oxygen gradient |
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Term
| do aerobes grow near the top or bottom of a tube? |
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Definition
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Term
| do anaerobes grow near the top or bottom of a tube? |
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Definition
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