Term
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Definition
| a dormant form of a bacterium that allows it to survive poor environmental conditions |
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Term
| What makes spores resistant to heat and chemicals? |
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Definition
| tough outer covering of protein keratin |
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Term
| Does keratin stain easily? |
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Definition
| no it resists staining, so extreme measures must be taken to stain |
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Term
| What stain is used in Schaeffer-Fulton method to stain spores? |
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Definition
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Term
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Definition
| malachite green is forced into the spore by steaming the bacterial emulsion |
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Term
| What are the three places in the cell that spores can be located? |
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Definition
| in the middle (central), at the end (terminal), between the end and middle (subterminal) |
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Term
| What are the 2 ways in which spores can be differentiated? |
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Definition
| based on: 1) shape (spherical or elliptical) 2) size |
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Term
| Why can vegetative cells and spore mother cells be decolorized with water and counterstained with safranin? |
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Definition
| because malachite green is water-soluble and has a low affinity for cellular material |
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Term
| What is the purpose of the spore stain? |
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Definition
| to detect the presence and location of spores in bacterial cells |
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Term
| Name two genera that produce spores. |
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Definition
-Bacillus-most are saprophytes, few are pathogens (cause of anthrax)
-Clostridium-most are saprophytes, 4 pathogens are fairly known (produce tetanus, botulism, gangrene, and colitis) |
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Term
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Definition
1. begin with heat-fixed emulsion
2. cover smear with bibulous paper, apply malachite green stain, steam for 5 min (keep paper moist w/stain)
3. grasp slide with slide holder, remove paper and dispose, gently rinse
4. counterstain w/safranin for 1 min, rinse
5. blot dry with bibulous paper, observe under oil immersion |
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Term
| What is another name for the Schaeffer-Fulton Method? |
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Definition
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Term
| In this lab, we are examining spores from what genera of bacteria? |
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Definition
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