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Micro Lab stains
Micro lab stains
11
Biology
Undergraduate 2
10/24/2006

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Cards

Term
what is the purple stain in a Gram stain? what is the red stain?
Definition
crystal violet and safranin
Term
what stain uses Nigrosin stain (india ink)? what is stained?
Definition
negative stain
background stained
Term
what stain used Malachite green?
Definition
spore stain
Term
what stain used Ziehl's carbolfuschin and methylene blue?
Definition
acid-fast stain
Term
what stains involved steam to trigger absorption of dyes?
Definition
spore stain and acid-fast
Term
what used in decolorizing step of acid-fast stain?
Definition
acid-alcohol
Term
what used in decolorizing step of Gram stain?
Definition
alcohol
Term
how long is each stain left on in a Gram stain?
Definition
crystal violet-1 min.
Iodine-1 min.
safranin-20 sec.
Term
what kind of organisms stain red with Gram stain?
Definition
gram (-)
Term
what color were spores in spore stain when malachite green and safranin used?
Definition
green
Term
what is secondary dye in acid-fast stain? primary?
Definition
methylene blue
Ziehl's carbolfushion
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