Term
| Best place to grow strict anaerobes? |
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Definition
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Term
| Aerotolerant and moderate anaerobes should be grown in what? |
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Definition
| Anaerobic-gas pack or biobag. |
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Term
| Microaerophiles should be grown in what? |
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Definition
| CO2 incubator or candle jar. |
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Term
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Definition
| Redox potential measured in microvolts. |
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Term
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Definition
| The substance is oxidized. |
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Term
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Definition
| The substance is very reduced (electron rich). |
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Term
| What microbes have the highest Eh? |
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Definition
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Term
| What microbes have the lowest Eh? |
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Definition
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Term
| What microbes have the second highest Eh? |
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Definition
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Term
| Names of two redox indicators: |
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Definition
Resazurin.
Methylene Blue. |
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Term
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Definition
Colorless - anaerobic conditions.
Pink - oxygenated conditions. |
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Term
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Definition
Colorless - anaerobic conditions.
Blue - oxygenated conditions. |
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Term
| The typical redox indicator in an anaerobe jar? |
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Definition
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Term
| Advantages of an anaerobe jar: |
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Definition
Convenient.
Inexpensive.
Mobile.
Doesn't take up much space. |
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Term
| Disadvantages of an anaerobe jar: |
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Definition
Limited space for plates and tubes.
Can't see plates without opening the system.
To work on the microbes they are exposed to oxygen. |
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Term
| Gas generator of an anaerobe jar contains what substances? |
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Definition
Sodium borohydrate.
Sodium bicarbonate.
Citric acid. |
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Term
| Do you add water to the gas generator or an anaerobe jar? |
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Definition
| Yes - H2 and CO2 will be released. |
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Term
| Redox indicator of a biobag: |
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Definition
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Term
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Definition
Inexpensive.
Mobile.
Doesn't take up much space.
Can see growth without opening system. |
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Term
| Disadvantages of a biobag: |
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Definition
Limited space for plates and tubes.
To transfer or stain, microbes are exposed to oxygen. |
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Term
| Advantages of an anaerobic glove box: |
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Definition
Lots of room.
Can see the plates.
Can work on microbes without oxygen exposure.
Never gets exposed to oxygen - strict anaerobes can be grown. |
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Term
| Disadvantages of an anaerobic glove box: |
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Definition
Expensive to set up and maintain.
Takes up lots of space.
Gets hot.
Hands get sweaty, need your own cotton liners.
Working for long periods of time. |
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Term
| Where are aerobic habitats? |
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Definition
Everywhere - especially soil.
Mucus membranes of animals.
Humans:
Oral cavity.
Colon.
Genitourinary tract.
Skin. |
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Term
| Blood flow in an anaerobic infection is typically ______: |
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Definition
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Term
| True or false: Anaerobic infections are typically poly-microbic? |
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Definition
| True. Anaerobic infections typically contain anaerobes, facultative anaerobes and microaerophiles. |
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Term
| Best specimen acquisition of anaerobes: |
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Definition
Aspirates and biopsies.
(Try to avoid normal flora) |
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Term
| Good specimen choices for anaerobe samples: |
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Definition
Body fluids.
Exudates from deep abscesses.
Transtracheal aspirates.
Direct lung aspirates.
Suprapubic punctures.
Tissue biopsies. |
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Term
| Least desirable specimen sample for anaerobes: |
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Definition
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Term
| Anaerobe specimen transportation should always be in a...? |
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Definition
| Closed system - excluding oxygen and avoiding drying. |
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Term
| Is a syringe or needle acceptable for anaerobe transportation? |
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Definition
| No. Due to sticks/injuries. |
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Term
| Clues for anaerobic infections: |
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Definition
Foul smelling odor*
Purulent appearance - presence of pus.
Necrotic tissue with gas.
Presence of sulfur granules.
Microbe present in primary Gram smear.
Does not grow aerobically. |
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Term
| Are primary Gram smears required for anaerobes? |
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Definition
Yes.
Use methanol fixation instead of heat.
Gram's iodine is mixed with Crystal Violet.
Use modified Safranin or extend counterstaining time. |
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Term
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Definition
Bi-plate: AnaBAP/BBE (Anaerobic Blood Agar Plate Bacteroides Bile Esculin)
Thioglycollate Broth.
KV Agar (Kanamycin-Vancomycin)
Chopped Meat Broth.
Egg Yolk Agar. |
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Term
| How long should Egg Yolk Agar plates be kept? |
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Definition
| Up to 7 days due to delayed reactions. |
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Term
| "Oil on water sheen" - What media? Why does it happen? |
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Definition
Egg Yolk Agar.
Happens due to lipase breaking down fats in egg yolks, causing iridescent sheen. |
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Term
| Lecithinase production - what media? why does it happen? |
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Definition
Egg Yolk Agar.
Happens because lecithinase degrades lecithin in egg yolks - produces an insoluble, opaque precipitation in the media surrounding growth. |
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