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Micro Exam 2 Chapter 6
Metabolic Regulation
28
Microbiology
Not Applicable
10/03/2021

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Term
DNA -> mRNA -> proteins
Definition
central dogma of life
Term
cytosol
Definition
where does both transcription and translation take place in prokaryotic cells
Term
metabolic/gene regulation
Definition
mechanisms for controlling which genes get expressed and at what levels
Term
promoter
Definition
RNA polymerase binding site where related genes found in a cluster on the chromosome are transcribed as a single unit
Term
operon
Definition
a cluster of genes under control of a single promoter
Term
activator
Definition
DNA binding proteins that increase transcription, bacteria use these to regulate gene expression
Term
repressor
Definition
DNA binding proteins that decrease transcription, bacteria use these to regulate gene expression
Term
transcription
Definition
regulatory molecules control the production of proteins by activating/blocking the process of ___________
Term
translation
Definition
regulatory molecules control the production of proteins by "sometimes" activating/blocking the process of ___________
Term
operator
Definition
site on bacterial DNA that regulatory molecules usually bind to to function
Term
lac operon
Definition
well studied and found in E. coli, also required for the metabolism of lactose
Term
glucose
Definition
simple sugar that is easier to breakdown; bacteria prefer to use this for energy
Term
lactose
Definition
disaccharide that is harder to break down
Term
inducible
Definition
term used to describe lac operon that is turned "off" but can be turned "on" by an inducer
Term
lac z, y, and A
Definition
structural genes a lac operon consists of
Term
promoter and operator
Definition
regulatory sequences a lac operon consists of along with a CAP site
Term
high glucose concentration in the environment but no lactose
Definition
repressor protein is formed and binds to the operator which blocks the RNA polymerase from binding to promoter inhibiting transcription
Term
no glucose in environment but has lactose
Definition
CAP binds to cAMP and RNA polymerase works more efficiently because it is free to bind to promoter
Term
allalactose
Definition
when too much lactose is present, it gets converted into another compound
Term
repressor
Definition
what allolactose binds to and causes to detach from the operator which frees RNA polymerase
Term
cAMP
Definition
regulates CAP site and made by E. coli when glucose levels are low
Term
CAP site
Definition
changes shape when bound to cAMP which makes it able to promote transcription
Term
trp operon
Definition
involved for the biosynthesis of the amino acid tryptophan; known as repressible and is usually "on"
Term
quorum sensing
Definition
how bacteria regulate their environment; found in both gram negative and gram positive bacteria and uses an auto inducer
Term
acyl homoserine lactones (AHL)
Definition
mostly found in gram negative bacteria and first class of autoinducers to be identified; binds to a kinase to induce transcription
Term
auto-inducing peptides
Definition
found in gram positive bacteria; smaller and uses a two-component system but can bind directly to a transcription factor instead of using a kinase
Term
virulence factor
Definition
controlled by quorum sensing; pathogen's ability to infect or damage host cell
Term
repressible
Definition
term used to describe try operon which is usually "on" but can be turned "off" by a corepressor
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