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Micro Exam 2 Chapter 5
Microbial growth and its control
57
Microbiology
Not Applicable
10/03/2021

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Term
increase in number of cells
Definition
In microbiology, growth is defined as an
Term
binary fission
Definition
Bacteria divide by a process known as
Term
septum
Definition
When a bacterial cell grows to twice their size and form a partition, it is known as
Term
two daughter cells
Definition
The partition from bacterial growth divides the cell into
Term
genetically identical
Definition
daughter cells relation to parent cells
Term
generation time
Definition
the time it takes for one bacterial cell to divide into two daughter cells
Term
environmental conditions and type of bacteria
Definition
generation time is controlled by
Term
every 20 minutes
Definition
e.coli division time
Term
every 10 minutes
Definition
clostridium parfringens division time
Term
exponential growth
Definition
growing bacterial population doubles at a regular interval when under favorable conditions, this is called
Term
typical bacterial growth curve
Definition
a fresh medium is inoculated with a given number of cells and the population growth is monitored over a period of time, plotting the data yields
Term
lag phase
Definition
phase of high metabolic activity immediately after inoculation when cell grows in size but NOT population
Term
exponential (log) phase
Definition
cell starts to divide rapidly; generation time equation can be used
Term
stationary phase
Definition
limited by food and other nutrients and by space and toxins; byproducts accumulate; growth plateaus
Term
death phase
Definition
cell population decreases
Term
chemically-defined (synthetic) medium
Definition
exact composition is known; uses pure biochemicals; minimum nutritional requirement of bacteria known
Term
complex (undefined) medium
Definition
composition not known; uses complex material of biological origin (beef extract, milk, yeast extract); cultivates unknown bacteria
Term
selective media
Definition
allows growth of one type of bacteria and suppresses others - ex. EMB - allows growth of gram negative bacteria
Term
differential media
Definition
differentiate between different species of bacteria - ex. blood agar differentiates between E. coli and staph epidermis
Term
enrichment culture
Definition
favor the growth of certain bacteria over others - ex. selenite f increases salmonella and decreases E. coli
Term
physical control methods
Definition
microbial growth can be controlled by heat and UV light
Term
chemical control
Definition
microbial growth control by antiseptic, disinfectants, and sanitizers
Term
heat
Definition
most common and easily available methods for controlling bacterial growth
Term
biomolecules - proteins
Definition
moist heat causes destruction of microorganisms by denaturation of these
Term
autoclave
Definition
most common method of moist sterilization
Term
steam chamber
Definition
samples are placed in a ______________ during autoclaving
Term
autoclave details
Definition
heated, closed chamber that steam forces air out of the vents or exhausts
Term
dry heat
Definition
incineration and flaming are examples of these sterilization methods
Term
pasteurization
Definition
developed by Louis Pasteur - uses heat to reduce the number of bacteria and all pathogenic bacteria are killed
Term
71 deg C for 15 seconds
Definition
time and temperature for pasteurization
Term
121 deg C after pressure of 15psi for 30 minutes
Definition
time and temperature for autoclave
Term
UV light radiation
Definition
can sterilize surfaces and transparent objects but damaging to plastics
Term
antiseptics
Definition
antimicrobial agents applied to living tissues/skin
Term
bactericidal
Definition
antiseptics that destroy bacteria
Term
bacteriostatic
Definition
antiseptics that inhibit bacterial growth
Term
hyperthermophile
Definition
bacteria that grows in 100 deg C and found in hot springs and deep sea hydrothermal vents
Term
thermophile
Definition
bacteria that grows between 45 and 100 deg C and found in hot springs as well as decaying plant matter like peat bogs and compost
Term
mesophile
Definition
bacteria that grows in moderate temperatures of -20 and 45 deg C and found widespread in nature
Term
psychrophiles
Definition
bacteria that grows in -10 deg C or lower and found in under sea ice in polar regions
Term
neutrophile
Definition
bacteria that grows in pH between 5.5 and 8
Term
acidophile
Definition
bacteria that grows in pH<5
Term
alkaliphile
Definition
bacteria that grows in pH>8
Term
halophile
Definition
bacteria that requires NaCl for growth
Term
mild halophile
Definition
bacteria that requires 1-6% salt
Term
moderate halophile
Definition
bacteria that requires 6-15% salt
Term
extreme halophile
Definition
bacteria that requires 15-30% NaCl for growth and found among archaea
Term
halotolerant
Definition
bacteria that grow at moderate NaCl concentrations but best in the absence of NaCl
Term
osmophile
Definition
organisms able to live in environments high in sugar
Term
xerophile
Definition
organisms that live in dry environments
Term
obligate (strict) aerobe
Definition
cannot grow without an abundant supply of oxygen
Term
microaerophile
Definition
bacteria that require a minimum level of oxygen for growth (1-10%) and well below the 21% found in the atmosphere
Term
obligate anaerobe
Definition
cannot survive in presence of oxygen
Term
facultative anaerobe
Definition
organisms that thrive in the presence of oxygen but also grow in its absence
Term
aerotolerant anaerobes
Definition
indifferent to the presence of oxygen; usually have fermentative metabolism but not harmed by the presence of oxygen
Term
sterilant
Definition
destroy all microorganisms including endospores
Term
disinfectant
Definition
chemicals that kill microorganisms but not necessarily endospores and used on surfaces
Term
sanitizer
Definition
reduces microbial numbers but do to sterilize
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