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Micro Ch 8
Microbiology Ch 8
41
Microbiology
Undergraduate 2
02/19/2016

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Term
Helicase
Definition
Enzyme for unzipping the DNA helix
Term
Primase
Definition
Enzyme used for synthesizing an RNA primer
Term
DNA polymerase III
Definition
Enzyme for adding bases to the new DNA chain; proofreading the chain for mistakes
Term
DNA polymerase I
Definition
Enzyme for removing primer, closing gaps, repairing mismatches
Term
Ligase
Definition
Enzyme for final binding of nicks in DNA during synthesis and repair
Term
Topoisomerases I and II
Definition
Enzymes for supercoiling and untangling
Term
Replication fork
Definition
Site where DNA splits apart
Term
Leading, lagging
Definition
DNA has a _____ strand and a _____ strand
Term
2
Definition
There are _____ replication forks for the replication of each chromosome
Term
DNA, RNA, protein
Definition
Information flows from _____ to ______ to _____
Term
Transcription
Definition
The first stage of gene expression. Master code of DNA used to synthesize an RNA molecule
Term
Translation
Definition
Transcribed RNA used to produce a protein (amino acid sequence)
Term
RNA viruses
Definition
Convert RNA to other RNA
Term
Retroviruses
Definition
Convert RNA to DNA
Term
Regulatory RNA
Definition
Regulate transcription and translation
Term
RNA polymerase
Definition
Large, complex enzyme that directs the conversion of DNA into RNA
Term
Template strand
Definition
Only one strand of DNA that contains meaningful instructions for synthesis of a functioning peptide
Term
Coding strand DNA, mRNA
Definition
These two are identical strands
Term
rRNA (ribosomal)
Definition
Makes up ribosomes
Term
tRNA (transfer)
Definition
Involved in binding to a specifying amino acid
Term
mRNA, tRNA, rRNA
Definition
RNA involved in translation
Term
mRNA, tRNA
Definition
_____ contains codons. _____ contains anticodons.
Term
Translation
Definition
All of the elements needed to synthesize a protein are brought together on ribosomes.
Term
Codon
Definition
Group of 3 base pairs which code for specific amino acids
Term
Methionine (AUG)
Definition
Start codon
Term
UAA, UAG, UGA
Definition
Stop codons
Term
2,3
Definition
# of hydrogen bonds for A and T, # of hydrogen bonds for G and C
Term
AUG, F methionine
Definition
Start mRNA codon
Term
Mutation
Definition
Any change to the nucleotide sequence in the genome
Term
Phenotype
Definition
Mutations are most noticeable when a genotypic change leads to a change in _____
Term
Spontaneous mutation
Definition
A random change in the DNA arising form errors in replication
Term
Induced mutations
Definition
Result from exposure to known mutagens
Term
Neutral mutations
Definition
Mutations that produce neither adverse or helpful changes
Term
Point mutations
Definition
Small mutations that affect only a single base on a gene
Term
Missense mutation
Definition
Mutation that leads to different amino acid. Creates a faulty, nonfunctional, or less functional protein.
Term
Nonsense mutation
Definition
Mutation that changes a normal codon into a stop codon
Term
Silent mutation
Definition
Mutation that alters the base but does not change the amino acid
Term
Back mutation
Definition
Mutation that occurs when a gene has undergone mutation reverses back to it's original base composition
Term
Frameshift mutation
Definition
Mutation that occurs when one or more bases are inserted into or deleted from a DNA strand, which leads to a change in the reading frame of mRNA
Term
Transcription
Definition
In bacteria, translation of mRNA starts while transcription is still occurring
Term
Operons
Definition
Coordinated set of genes only found in bacteria that act as a single unit.
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