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| science of classification |
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Swedish botanist credited w/founding science of taxonomy originated binomial nomenclature |
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| system of taxonomy developed by Linnaeus in which each organism is assigned a genus and specific epithet |
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| A name of a bacterium often reveals what? |
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| its shape, where it is found, what nutrients it needs, who discovered it, what disease it causes |
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| supgroup of a species with one or more characteristics that distinguish it |
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| How is a specific strain identified? |
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by name, number, or letter following specific epithet Ex. E. coli O157:H7 |
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| 3 reasons why taxonomy is important |
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1) identification of organisms 2) classification 3) gives info for evolutionary change |
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| tool of taxonomy that presents an "either-or" choice system to classify organisms |
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| 1) goal is to classify by phylogenetic relationships but this is difficult b/c evolution is continuous and rapidly occurring, and knowledge of evolutionary history of bacteria is incomplete |
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1. Plantae 2. Fungi 3. Animalia 4. Protista 5. Monera |
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| 4 characteristics of life |
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1. all organisms composed of cells 2. all carry out certain functions (metabolism) 3. cell is basic unit of life 4. all cells bound by cell membrane, have DNA, ribosomes, lipids, and carbs |
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unicellular prokaryotes lack true nuclei lack membrane bound organelles DNA has little/no protein reproduce by binary fission 3 types: Eubacteria, Cyanobacteria, Archaeobacteria |
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diverse group (algae, protozoa, euglena) unicellular eukaryotes true nucleus membrane bound organelles some cause disease do not develop from embryo like plants/animals or spores like fungi |
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| Give examples of Protists that cause disease. |
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Plasmodium-->malaria Amoeba-->dysentary |
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unicellular & multicellular eukaryotes form spores not seeds some cause disease |
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| Give examples of Fungi that cause disease |
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Candida albicans-->yeast infection Aspergillus niger-->black mold Cryptococcus neoformans |
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multicellular eukaryotes photosynthesize have chlorophyll some produce antimicrobial substances some susceptible to fungal, viral, bacterial infections |
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mostly macroorganisms, but still studied b/c can be carries of microorganisms all develop from zygotes multicellular eukaryotes some parasites |
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| Give examples of Animalia that are parasites. |
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helminth worms (tapeworms, flukes, roundworms) arthropods (ticks, mites, lice, fleas) |
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| Bacteria, Archaea, Eukarya |
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fossilized photosynthetic prokaryotes that appear as masses of cells or microbial mats studying these shows that 3 separate domains arose 3 billion years ago |
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| tree versus shrub view of evolution |
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proposed by W. Ford Doolittle said that evolution had many roots rather than 1 ancestral line and branches crisscross/merge, which occurred through lateral gene transfer |
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acellular infectious agents smaller than cells have nucleic acid (DNA or RNA) nonliving cause disease not placed in a kingdom |
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| What types of characteristics are viruses classified by? |
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| shape, type of n.a, presence of envelope, enzymes, and tail structure |
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| Why is virology important? |
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1) techniques are derived from micro techniques 2) viruses are a concern to health scientist b/c of disease |
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| Why is the classification based on morphology not helpful for prokaryotes? |
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| prokaryotes have few structural characteristics and they are rapidly changing |
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| comparison of organisms based on quantitative assessment of large number of characteristics |
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| taxonomic methods for bacteria |
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numerical taxonomy genetic homology ribosomal gene sequencing immunology phage typing |
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| What are some methods of studying genetic homology of bacteria for taxonomic purposes? |
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direct methods: base composition comparison, DNA/RNA sequencing, DNA hybridization indirect methods: protein profiling |
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| base composition comparison |
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Definition
a direct method of studying genetic homology for taxonomic purposes comparing percentages of bases present in DNA |
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a direct method of studying genetic homology for taxonomic purposes probes are used to identify specific DNA sequences, to which they attach and help in identification of specimen |
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| SS DNA fragments that have sequences complementary to those being sought |
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a direct method of studying genetic homology for taxonomic purposes process in which DS of DNA of each of 2 organisms are split apart and allowed to anneal degree of annealing is directly proportional to quantity of identical DNA |
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an indirect method of studying genetic homology for taxonomic purposes lab-prepared pattern of proteins found in a cell, which are unique to each organism, is prepared by electrophoresis bands at same location indicate same protein is present in 2 cells |
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| ribosomal gene sequencing |
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| taxonomic method in which degree of similarity in 16S rRNA sequences indicates evolutionary relatedness |
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| immunology as a taxonomic method |
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monoclonal antibodies are studied, which bind to specific protein on cell surface if they bind to more than 1 organism, organisms have protein in common |
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| phage typing as a taxonomic method |
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use of bacteriophages, which cause holes in bacterial lawn, allowing identification of a strain strains lysed by same phages are closely related |
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| process in which descendants of a common ancestor species undergo sufficient change to be identified as separate species |
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| collection of strains that share many common features and differ significantly from other strains |
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original reference strain of a bacterial species, descendants of a single isolation in pure culture namebearer of species, preserved in culture collections |
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internationally recognized reference for bacterial taxonomy uses dichotomous key and physical characteristics does not present accurate picture of evolutionary relationships, but instead practical groups of bacteria for easy identification |
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| problems with bacterial taxonomy |
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too little is known about evolutionary relationships some groups contain unusual organisms |
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