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MICR33
Chapter 4-Prokaryotes
58
Microbiology
Not Applicable
06/26/2012

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Term
Prokaryote
Definition
Comes from the Greek words for prenucleus
Term
Eukaryote
Definition
Comes from the Greek words for true nucleus.
Term
Prokaryote
Definition
One circular chromosome, not surrounded by a nuclear membrane, no histones, no membrane bound organelles, bacteria: peptidoglycan cell walls, archaea: pseudomurein cell walls, binary fission
Term
Eukaryote
Definition
Paired chromosomes surrounded by a nuclear membrane, histones, membrane bound organelles, polysaccharide cell walls, mitotic spindle.
Term
Prokaryotic cell shape
Definition
Average size:
0.2-1.0 microns x 2-8 microns
Monomorphic
Few are pleomorphic
Term
Glycocalyx
Definition
Outside cell wall
Usually sticky
Capsule: neatly organized
Slime layer: unorganized
Term
Flagella
Definition
-outside cell wall
-made of chains of flagellin
-attached to a protein hook
-anchored to the wall an membrane by the basal body
Term
Motile cells
Definition
Rotate flagella to run or tumble
Move toward or away from stimuli
Flagella proteins are H antigens
Term
Axial filaments
Definition
Also called endoflagella, in spirochetes, anchored at one end of cell, rotation causes cell to move.
Term
Fimbriae
Definition
Fimbriae allow attachment
Term
Pili
Definition
Pili facilitate transfer of DNA from one end of cell to another, gliding motility, twitching motility
Term
Peptidoglycan
Definition
Polymer of disaccharide
N-acetylglucosamine (NAG)
N-acetylmuramic acid (NAM)
Term
Peptidoglycan in Gram-Positive Bacteria
Definition
Linked by polypeptides at NAM
Term
Gram-Positive Cell Wall
Definition
Thick peptidoglycan
Teichoic acids:
- lipoteichoic acid links to plasma membrane
-wall Teichoic acid links to peptidoglycan
-may regulate movement of cations and provide antigenic specificity
Polysaccharides covering the cell wall may provide antigenic specificity as well
Term
Gram-Negative Cell Wall
Definition
Thin peptidoglycan, outer membrane, periplasmic space
Term
Gram-Negative outer membrane
Definition
Lipopolysaccharides, lipoproteins, phospholipids
Forms the periplasm between the outer membrane and the plasma membrane. Protection from phagocytes, complement, and antibiotics
Term
Gram stain mechanism
Definition
Crystal violet-iodine crystals form in cell
-gram positive: alcohol dehydrates peptidoglycan, CV-I crystals do not leave
-gran-negative: alcohol dissolves outer membrane and leaves holes in peptidoglycan, CV-I washed out
Term
Gram positive cell wall
Definition
2-ring basal body, disrupted by lysozyme, penicillin sensitive
Term
Gram-negative cell wall
Definition
4-ring basal body, endotoxin, tetracycline sensitive
Term
Atypical cell walls
Definition
Acid-fast cell walls
Waxy lipid (mycolic acid) bound to peptidoglycan
Mycobacterium
Nocardia
Term
Damage to cell wall
Definition
Lysozyme digests disaccharide in peptidoglycan, penicillin inhibits peptide bridges in peptidoglycan
Term
Protoplast
Definition
Wall-less cell with a plasma membrane intact
Term
Spheroplast
Definition
Gram-negative cell with a remnant of the outer wall left plus an intact plasma membrane
Term
Protoplasts an spheroplasts are susceptible to osmotic lysis
Definition
That's bad
Term
L forms
Definition
Are wall-less cells that swell into irregular shapes (still live and divide)
Term
The plasma membrane
Definition
Phospholipid bilayer, peripheral proteins and integral proteins, transmembrane proteins
Term
Fluid mosaic model
Definition
Membrane'sas viscous as olive oil
Proteins move to function
Phospholipids rotate and move laterally
Term
Selective permeability
Definition
Allows passage of some molecules
Term
Enzymes in plasma membrane
Definition
For ATP production
Term
Chromatophore and thylakoids
Definition
Photosynthetic pigments on foldings of plasma membrane
Term
Damage to plasma membrane
Definition
By alcohols, quaternary Ammonium, polymyxin antibiotics cause leakage of cell contents
Term
Simple diffusion
Definition
Movement of a solute from high to low concentration
Term
Facilitated diffusion
Definition
Solute combines with a transporter protein in the membrane
Term
Facilitated diffusion
Definition
Solute combines with a transporter protein in the membrane
Term
Osmosis
Definition
The movement of water across a selectively permeable membrane from an area of high water concentration to an area of low water concentration
Term
Osmotic pressure
Definition
The pressure needed to stop the movement of water across the membrane.
Term
Aquaporin
Definition
The channel through which water moves through the plasma membrane
Term
Isotonic solution
Definition
Equal concentration
Term
Hypotonic
Definition
Water moves into cell. Might burst.
Term
Osmotic lysis
Definition
Cell bursts from osmosis
Term
Hypertonic solution
Definition
Water moves out of the cell causing cytoplasm to shrink
Term
Plasmolysis
Definition
Cell shrinks
Term
Active transport
Definition
Requires a transporter protein and energy in the form of ATP
Term
Group translocation
Definition
Requires a transporter protein and PEP (a special form of active transport in prokaryotes. Substances are altered as they are transported.
Term
Cytoplasm
Definition
The substance inside the plasma membrane (not a void, it is a very rich environment)
Term
The nucleoid
Definition
Bacterial chromosome. No boundary to mark nucleoid location
Term
PEP
Definition
Phosphoenol pyruvate
Term
The prokaryotic ribosome
Definition
Protein synthesis.
Complete 70S structure.
Made of 1 small subunit (30S) and one large unit(50S).
S stands for Svedberg unit.
Ribosome is non membranous.
It is made of protein and RNAs
Term
Meta chromatic granules (volutin)
Definition
Phosphate reserves, for generating ATP
Term
Polysaccharide granules
Definition
Energy reserves
Term
Lipid inclusions and sulfur granules
Definition
Energy reserves
Term
Carboxysomes
Definition
Ribulose 1,5-diphosphate carboxylase for CO2 fixation
Term
Gas vacuoles
Definition
Protein-covered cylinders
Help organisms float for photosynthetic purposes
Term
Magnetosomes
Definition
Iron oxide (destroys H2O2)
Hydrogen peroxide is toxic, this helps rid of it.
Term
Svedberg unit
Definition
"S" in ribosomes.
Accounts for size, weight, and shape.
A unit of sediments
Term
Endospores
Definition
Resting cells. Resistant to desiccation, heat, chemicals.
-Bacillus(genus)
-Clostridium(genus)
Term
Sporulation
Definition
Endospore formation
Term
Germination
Definition
Return to vegetative state
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